- Clone
- 13A3-1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Signal transducer and activator of transcription 3, Acute-phase response factor, APRF, HIES
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![13A3-1_AF647_STAT3_Phospho_Tyr705_Antibody_1_032416 13A3-1_AF647_STAT3_Phospho_Tyr705_Antibody_1_032416](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/13A3-1_AF647_STAT3_Phospho_Tyr705_Antibody_1_032416.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human whole blood was stimulated with (top), or without (bottom) IL-6 for 15 minutes, and then treated with RBC Lysis/Fixation Solution (10X), permeabilized with True-Phos™ Perm Buffer, then stained with CD3 PE and STAT3 Phospho (Tyr705) (clone 13A3-1) Alexa Fluor® 647. -
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
651007 | 25 tests | 137€ | ||||
651008 | 100 tests | 306€ |
Tyrosine phosphorylation of STAT3 at Tyr705 occurs in response to LIF, IL-6, leptin, OSM, EGF, PDGF, and HGF. It plays a key role in cell growth and apoptosis through mediating expression of a variety of genes in response to the stimuli.
Product DetailsProduct Details
- Reactivity
- Human,Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH conjugated modified synthetic peptide
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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ICFC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Whole Blood Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
The STAT3 Phospho (Tyr705) antibody recognizes the regulatory tyrosine phosphorylation of human STAT3 protein and has been shown to be useful for Western blotting.
- Product Citations
- RRID
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AB_2572085 (BioLegend Cat. No. 651007)
AB_2572086 (BioLegend Cat. No. 651008)
Antigen Details
- Structure
- STAT3 is a 770 amino acid protein of 88 kD. It consists of a DNA binding domain, a SH2 domain, a regulatory tyrosine responsible for binding of SH2 domain, and a C-terminal transactivation domain.
- Distribution
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Ubiquitous.
- Function
- STAT3 is tyrosine phosphorylated by receptor kinases in response to a variety of cytokines and growth factors. It forms homo- or heterodimer with STAT1 when tyrosine is phosphorylated, and then translocates to nucleus, acting as a transcription regulator. It is also essential for the differentiation of TH17 cells, which is involved in autoimmune diseases.
- Cell Sources
- Cytoplasm. Translocate to nucleus in response to tyrosine phosphorylation.
- Cell Type
- Embryonic Stem Cells, Neural Stem Cells
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Synaptic Biology, Transcription Factors
- Molecular Family
- Phospho-Proteins
- Antigen References
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1. Akira S, et al. 1994. Cell 77:63.
2. Zhang X, et al. 1995. Science 267:1990.
3. Sanchez-Margalet V, et al. 2001. Cell. Immunol. 211:30.
4. Simon A, et al. 2000. Science 290:144.
5. Hoey T, et al. 1999. Adv. Immunol. 71:145. - Regulation
- The small GTPase Rac1 binds and regulates activity of STAT3.
- Gene ID
- 6774 View all products for this Gene ID
- UniProt
- View information about STAT3 Phospho Tyr705 on UniProt.org
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