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Alexa Fluor® 700 anti-human CD8 Antibody

Pricing & Availability
Clone
SK1 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Leu2
Isotype
Mouse IgG1, κ
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Product Citations
publications
SK1_AF700_CD8_Antibody_FC_111213.jpg
Human peripheral blood lymphocytes were stained with CD8 (clone SK1) Alexa Fluor® 700 (filled histogram) or mouse IgG1, κ Alexa Fluor® 700 isotype control (open histogram).
  • SK1_AF700_CD8_Antibody_FC_111213.jpg
    Human peripheral blood lymphocytes were stained with CD8 (clone SK1) Alexa Fluor® 700 (filled histogram) or mouse IgG1, κ Alexa Fluor® 700 isotype control (open histogram).
See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability Save
344723 25 tests 90€
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344724 100 tests 221€
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Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics8, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)9,10. This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1981. J. Exp. Med. 153:310.
  2. Campanelli R, et al. 2002. Intl. Immunol. 14:39.
  3. Evans RL, et al. 1981. Immunol. 78:544.
  4. Wooldridge L, et al. 2005. J. Bio. Chem. 280:27491.
  5. Ch'el IL, et al. 2011. J Exp Med. 208:633. PubMed
  6. Carbone A, et al. 1999. Blood 93:2319. (IHC-F)
  7. Ahmed A, et al. 2001. J. Pathol. 193:383. (IHC)
  8. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  9. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  10. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Yarzabek B et al. 2018. eLife. 7 pii: e34961. PubMed
  2. Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
  3. Salvany‐Celades M et al. 2019. Cell Rep. 27(9):2537-2547 . PubMed
  4. Coufal S, et al. 2019. Cells. 8. PubMed
  5. Serra EG, et al. 2020. Nat Commun. 11:995. PubMed
  6. Charpentier JC, et al. 2020. Nat Commun. 11:180. PubMed
  7. Crespo ÂC, et al. 2020. Cell. 182(5):1125-1139. PubMed
  8. Ott PA, et al. 2020. Cell. 183(2):347-362.e24. PubMed
  9. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  10. Ma X, et al. 2021. Cell Metabolism. 33(5):1001-1012.e5. PubMed
  11. Neidleman J, et al. 2020. Cell Rep Med. 100081:1. PubMed
  12. Cohen CA, et al. 2021. Nat Commun. 12:4678. PubMed
  13. FitzPatrick MEB, et al. 2021. Cell Rep. 34:108661. PubMed
  14. Williamson LM, et al. 2021. NPJ Precis Oncol. 5:103. PubMed
  15. Vanoni G, et al. 2022. Bio Protoc. 218:. PubMed
  16. Pellett Madan R, et al. 2020. Clin Transplant. 34:e14021. PubMed
  17. Bradley D, et al. 2022. Nat Commun. 13:5606. PubMed
  18. Zhong W, et al. 2022. Front Immunol. 13:1001255. PubMed
  19. Ackerley CG, et al. 2022. Front Immunol. 13:972170. PubMed
  20. Jaiswal A, et al. 2022. Cancer Cell. 40:524. PubMed
  21. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  22. Vyasamneni R, et al. 2023. Cell Rep Methods. 3:100388. PubMed
RRID
AB_2562789 (BioLegend Cat. No. 344723)
AB_2562790 (BioLegend Cat. No. 344724)

Antigen Details

Structure
Ig superfamily, homodimer or heterodimer with CD8b, 32-34 kD
Distribution

Majority of thymocytes, T cell subset, NK cells

Function
MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor
MHC Class I molecules
Cell Type
NK cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
925 View all products for this Gene ID
UniProt
View information about CD8 on UniProt.org
Go To Top Version: 4    Revision Date: 07/13/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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