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Anti-HA Tag Antibody (Previously Covance catalog# PRB-101C)

Pricing & Availability
Clone
Poly9023 (See other available formats)
Regulatory Status
RUO
Other Names
HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
Previously
Covance Catalog# PRB-101C
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly9023_Purified_HA-Tag_Antibody_WB_032615
Western blot analysis of HA-Tag antibody to Postitag control protein.
  • Poly9023_Purified_HA-Tag_Antibody_WB_032615
    Western blot analysis of HA-Tag antibody to Postitag control protein.
Cat # Size Price Quantity Check Availability Save
923501 200 µL 313€
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923502 500 µL 492€
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Description

Influenza Hemagglutinin (HA) is a viral membrane glycoprotein responsible for binding of the virus to the cells expressing sialic acid-containing surface receptors, such as erythrocytes and the cells in the upper respiratory tract. The hemagglutinin epitope, YPYDVPDYA, has been widely used as a tag in recombinant protein expression vectors.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Polyclonal antibody HA.11 was raised against the twelve amino acid sequence CYPYDVPDYASL.
Preparation
Serum
Storage & Handling
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
Application

WB, IP
Recommended Usage

The optimal working dilution should be determined for each specific assay condition.
• WB: 1:1,000*
• IP: 1:150

Application Notes

This antibody is effective in immunoblotting (WB) and immunoprecipitation (IP) of tagged proteins.

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

This antibody recognizes the same hemagglutinin flu epitope YPYDVPDYA as the monoclonal antibody, HA.11 clone 16B12. This polyclonal antibody facilitates epitope tag experiments in murine systems and is useful in experiments involving two proteins labeled with two different tags.


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Barrott JJ, et al. 2011. PNAS. 108: 12752. (WB, IP)
  2. Pecot MY, Malhotra V. 2004. Cell. 116:99.
  3. Delmas C, et al. 2003. J Biol Chem. 278:12443. (IF)
  4. Caplan S, et al. 2001. J Cell Biol. 154:109. (IF, WB)
  5. Yeo C, Whitman M. 2001. Mol Cell. 7:949.
  6. Sander TL, et al. 2000. J Biol Chem. 275:12857. (WB)
  7. Bosshart H, et al. 1994. J Cell Biol. 126:1157. (IF, IP)
Product Citations
  1. Trautz B, et al. 2016. J Virol. 90(23):10915-10927. PubMed
  2. Zhu Z, et al. 2016. J Virol. 90(24):11106-11121. PubMed
  3. Villeneuve J, et al. 2017. Mol Biol Cell. 28(1):141-151. PubMed
  4. Ting Y, et al. 2017. J Biol Chem. 292(2):585-596. PubMed
  5. Li J, et al. 2021. Med (N Y). 0.152083333. PubMed
  6. Zeng J, et al. 2020. Cell Stem Cell. 27(4):618-632.e9. PubMed
  7. Hao L, et al. 2021. Cell Prolif. :e12994. PubMed
  8. Tazat K, et al. 2021. Mol Biol Cell. 32:605. PubMed
  9. Khan I, et al. 2022. Cell Rep. 38:110322. PubMed
  10. Khan I, et al. 2021. Methods Mol Biol. 2262:281. PubMed
  11. Wang S, et al. 2022. Methods Mol Biol. 2473:181. PubMed
  12. Ithinji DG, et al. 2022. NPJ Vaccines. 7:166. PubMed
  13. Li W, et al. 2023. Life Sci Alliance. 6: . PubMed
  14. Wallon L, et al. 2022. Proc Natl Acad Sci U S A. 119:e2204481119. PubMed
RRID
AB_2565437 (BioLegend Cat. No. 923501)
AB_2565438 (BioLegend Cat. No. 923502)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about HA Tag on UniProt.org
Go To Top Version: 6    Revision Date: 04/27/2023

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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