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APC anti-human CD8 Antibody

Pricing & Availability
Clone
SK1 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Leu2
Isotype
Mouse IgG1, κ
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Product Citations
publications
SK1_APC_040810
Human peripheral blood lymphocytes stained with SK1 APC
  • SK1_APC_040810
    Human peripheral blood lymphocytes stained with SK1 APC
See APC spectral data
Cat # Size Price Quantity Check Availability Save
344721 25 tests 106€
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344722 100 tests 260€
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Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics8, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)9,10. This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1981. J. Exp. Med. 153:310.
  2. Campanelli R, et al. 2002. Intl. Immunol. 14:39.
  3. Evans RL, et al. 1981. Immunol. 78:544.
  4. Wooldridge L, et al. 2005. J. Bio. Chem. 280:27491.
  5. Ch'el IL, et al. 2011. J Exp Med. 208:633. PubMed
  6. Carbone A, et al. 1999. Blood 93:2319. (IHC-F)
  7. Ahmed A, et al. 2001. J. Pathol. 193:383. (IHC)
  8. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  9. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  10. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Chheda ZS, et al. 2018. J Exp Med. 9:19490. PubMed
  2. Wang F, et al. 2018. Oncogenesis. 7:41. PubMed
  3. Su S et al. 2018. Cell. 175(2):442-457 . PubMed
  4. Horn LA, et al. 2017. Oncotarget. 8:57964. PubMed
  5. Li B, et al. 2019. Oncogenesis. 8:17. PubMed
  6. Wang J, et al. 2019. Oncol Lett. 4.948611111. PubMed
  7. Forsberg EMV, et al. 2019. Cancer Res. 79:899. PubMed
  8. Zhao L, et al. 2020. Mol Ther Oncolytics. 16:262. PubMed
  9. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
  10. Oliveira A, et al. 2013. Immunology. 140:47. PubMed
  11. Leitner J, et al. 2015. J Immunol. 195: 477 - 487. PubMed
  12. Javed A, et al. 2015. PLoS One. 10: 0142086. PubMed
  13. Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed
  14. Tripathi D, et al. 2016. Nat Commun. 7:13896. PubMed
  15. Wahl S, et al. 2016. Nature. 541:81-86. PubMed
  16. Walker‐Sperling VE et al. 2017. EBioMedicine. 16:141-149 . PubMed
  17. Tao L, et al. 2020. Cancers (Basel). 12:00. PubMed
  18. Gruber T, et al. 2020. JCI Insight. 5:00. PubMed
  19. Kang L, et al. 2020. Exp Hematol Oncol. 9:11. PubMed
  20. Hoseini SS, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(5):. PubMed
  21. Le J, et al. 2020. Immunity. 52(6):1105-1118.e9. PubMed
  22. Roudko V, et al. 2020. Cell. 183(6):1634-1649.e17. PubMed
  23. Clayton KL, et al. 2021. Cell Host Microbe. 29(3):435-447.e9. PubMed
  24. Loo Yau H, et al. 2021. STAR Protocols. 2(2):100549. PubMed
  25. Reif T, et al. 2021. Cell Reports Medicine. 2(6):100317. PubMed
  26. Grant RA, et al. 2021. Nature. 590(7847):635-641. PubMed
  27. Fierle JK, et al. 2021. Cell Reports Medicine. 2(8):100362. PubMed
  28. Su Y, et al. 2020. Cell. 1479:183. PubMed
  29. Thiel BA, et al. 2021. PLoS One. 16:e0249477. PubMed
  30. Zheng Z, et al. 2021. Biomed Res Int. 2021:5535578. PubMed
  31. Tang L, et al. 2021. Oncol Lett. 22:605. PubMed
  32. Zou F, et al. 2021. Mol Ther. 29:1794. PubMed
  33. Paul S, et al. 2021. Sci Transl Med. 13:. PubMed
  34. Kumar A, et al. 2020. Bio Protoc. 10:e3675. PubMed
  35. Egedal JH, et al. 2021. Mucosal Immunol. 14:1203. PubMed
  36. Bear AS, et al. 2021. Nat Commun. 12:4365. PubMed
  37. Zhan Y, et al. 2021. JCI Insight. 6:. PubMed
  38. Kasper M, et al. 2021. Elife. 10:. PubMed
  39. Bhargavan B, et al. 2021. Mol Neurodegener. 16:78. PubMed
  40. Jing L, et al. 2020. Cancer Immunol Res. 8:648. PubMed
  41. Feng Y, et al. 2022. EClinicalMedicine. 43:101226. PubMed
  42. Mudd PA, et al. 2022. Cell. 185:603. PubMed
  43. Qiu C, et al. 2022. Front Immunol. 12:764949. PubMed
  44. Lugo-Reyes SO, et al. 2021. J Clin Immunol. 41:1291. PubMed
  45. Wu Y, et al. 2021. Cancer Gene Ther. Online ahead of print. PubMed
  46. Massafra V, et al. 2021. J Immunol. 207:493. PubMed
  47. Bonte S, et al. 2021. Oncoimmunology. 10:1954800. PubMed
  48. Nguyen NT, et al. 2021. Nat Nanotechnol. 16:1424. PubMed
  49. Deng J, et al. 2021. J Leukoc Biol. 110:1171. PubMed
  50. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  51. Qin L, et al. 2022. Sci Adv. 8:eabn3774. PubMed
  52. Tessaro FHG, et al. 2022. Cell Rep. 39:110977. PubMed
  53. Ma T, et al. 2022. Elife. 11: . PubMed
  54. Li K, et al. 2022. Cancer Cell. 40:1374. PubMed
  55. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  56. Kim S, et al. 2023. Front Immunol. 14:1062365. PubMed
  57. Cheng K, et al. 2023. Int J Mol Sci. 24:. PubMed
  58. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
  59. Li L, et al. 2023. Heliyon. 9:e14219. PubMed
  60. Xiao C, et al. 2023. Nat Aging. 3:418. PubMed
RRID
AB_2075390 (BioLegend Cat. No. 344721)
AB_2075388 (BioLegend Cat. No. 344722)

Antigen Details

Structure
Ig superfamily, homodimer or heterodimer with CD8b, 32-34 kD
Distribution

Majority of thymocytes, T cell subset, NK cells

Function
MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor
MHC Class I molecules
Cell Type
NK cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
925 View all products for this Gene ID
UniProt
View information about CD8 on UniProt.org

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Go To Top Version: 4    Revision Date: 07/13/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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