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APC anti-human CD86 Antibody

Pricing & Availability
Clone
IT2.2 (See other available formats)
Regulatory Status
RUO
Workshop
VI CD86.8
Other Names
B7-2, B70
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
IT2dot2_APC_111607
Human peripheral blood monocytes stained with IT2.2 APC
  • IT2dot2_APC_111607
    Human peripheral blood monocytes stained with IT2.2 APC
See APC spectral data
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305411 25 tests 120€
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305412 100 tests 252€
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Description

CD86 is an 80 kD immunoglobulin superfamily member also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, monocytes/macrophages, dendritic cells, and astrocytes. CD86, along with CD80, is the ligand of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce costimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can bind to CD152 as well, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, Western blotting3, and blocking of T cell activation2,4,5. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 305449 & 305450).

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Dieu M. 1998. J. Exp. Med. 188:373. (Block)
  3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
  4. Jeannin P, et al. 1999. J. Immunol. 162:2044. (Block)
  5. Kapsogeorgou EK, et al. 2001. J. Immunol. 166:3107. (Block)
  6. Geissmann F, et al. 2001. Blood 97:1241. (IHC)
Product Citations
  1. Hazeldine J, et al. 2017. PLoS Med. 10.1371/journal.pmed.1002338. PubMed
  2. Meng Y,et al. 2017. Cell Death Dis. . 10.1038/cddis.2017.505. PubMed
  3. Ackermann M, et al. 2018. Nat Commun. 9:5088. PubMed
  4. Castellaro AM, et al. 2019. Cancers (Basel). 11:. PubMed
  5. Cassetta L et al. 2019. Cancer Cell. 35(4):588-602 . PubMed
  6. Celis‐Gutierrez J et al. 2019. Cell Rep. 27(11):3315-3330 . PubMed
  7. Ong SM, et al. 2019. Front Immunol. 10:1761. PubMed
  8. Longbrake EE, et al. 2018. Mult Scler. 24:728. PubMed
  9. Chometon TQ, et al. 2020. PLoS One. 15:e0231132. PubMed
  10. Kim SE, et al. 2019. PLoS One. 14:e0223590. PubMed
  11. Herath S, et al. 2014. PLoS One. 9:88327. PubMed
  12. Nastasi C, et al. 2015. Sci Rep. . PubMed
  13. Nowak A, et al. 2015. Ann Oncol. 26: 2483 - 2490. PubMed
  14. Hu X, et al. 2016. PLoS One. 11: 0152368. PubMed
  15. Kotsiou E, et al. 2016. Blood. 128: 72 - 81. PubMed
  16. Siedlik J, et al. 2017. J Immunol Methods. 10.4049/jimmunol.1700003. PubMed
  17. Papalexi E, et al. 2021. Nat Genet. 53:322. PubMed
  18. Pinto SM, et al. 2021. Front Immunol. 12:679458. PubMed
  19. Vereertbrugghen A, et al. 2021. Front Oncol. 11:598319. PubMed
  20. Zhu F, et al. 2022. Front Immunol. 13:879575. PubMed
  21. Muto S, et al. 2021. Oncol Lett. 21:203. PubMed
  22. Arcangeli S, et al. 2022. J Clin Invest. 132: . PubMed
  23. Amo L, et al. 2022. Front Immunol. 13:835527. PubMed
  24. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
  25. Perera MR, et al. 2022. Int J Mol Sci. 23:. PubMed
  26. Xu G, et al. 2022. Cell Death Dis. 13:888. PubMed
  27. Tu J, et al. 2022. Cancer Sci. 113:2496. PubMed
  28. Taffoni C 2022. The EMBO Journal. e111961. PubMed
  29. Rodriguez-Perdigon M, et al. 2023. Front Bioeng Biotechnol. 11:1159819. PubMed
RRID
AB_493232 (BioLegend Cat. No. 305411)
AB_493231 (BioLegend Cat. No. 305412)

Antigen Details

Structure
Ig superfamily, single-chain transmembrane glycoprotein, 80 kD
Distribution

Monocytes/macrophages, activated B cells and T cells, dendritic cells

Function
T cell costimulation
Ligand/Receptor
CD28, CD152
Cell Type
B cells, Dendritic cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Hathcock K, et al. 1996. Adv. Immunol. 62:131.
2. June C, et al. 1994. Immunol. Today 15:321.

Gene ID
942 View all products for this Gene ID
UniProt
View information about CD86 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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