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APC anti-human IFN-γ Antibody

Pricing & Availability
Clone
4S.B3 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype
Mouse IgG1, κ
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Product Citations
publications
4S.B3_APC_IFNg_Antibody_FC_1_010714
PMA+ionomycin stimulated (6 hours) human peripheral blood lymphocytes (in the presence of monensin) were stained with CD3 PE, then fixed with Fixation Buffer (Cat# 420801), and permeabilized with Permeabilization Wash Buffer (Cat# 421002). Cells were then stained with IFN-γ (clone 4S.B3) APC (top) or mouse IgG1, κ APC isotype control (bottom).
  • 4S.B3_APC_IFNg_Antibody_FC_1_010714
    PMA+ionomycin stimulated (6 hours) human peripheral blood lymphocytes (in the presence of monensin) were stained with CD3 PE, then fixed with Fixation Buffer (Cat# 420801), and permeabilized with Permeabilization Wash Buffer (Cat# 421002). Cells were then stained with IFN-γ (clone 4S.B3) APC (top) or mouse IgG1, κ APC isotype control (bottom).
  • 4S.B3_APC_IFNg_Antibody_FC_2_010714
See APC spectral data
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502511 25 tests 96€
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502512 100 tests 228€
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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partially purified, native human IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA or ELISPOT Detection5: The biotinylated 4S.B3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified NIB42 antibody (Cat. No. 502402/502404) or purified MD-1 antibody (Cat. No. 507502/507513) as the capture antibody.
Flow Cytometry3,4,6-8: The fluorochrome-labeled 4S.B3 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ -producing cells within mixed cell populations.
Additional reported applications (for the relevant formats) include: neutralization1,2, Western blotting, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated tissue sections, and immunocytochemistry. The 4S.B3 antibody can neutralize the bioactivity of natural or recombinant IFN-γ.
Note: For testing human IFN-γ in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430101 to 430106) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Meager A, et al. 1984. J. Interferon Res. 4:619. (Neut)
  2. Meager A, 1987. Lymphokines and Interferons:A Practical Approach. IRL Press Ltd, Oxford, p. 105. (Neut)
  3. Sester M, et al. 2002. J. Virol. 76:3748. (ICFC)
  4. Infante-Duarte C, et al. 2000 J. Immunol. 165:6107. (ICFC)
  5. Goodier M, et al. 2000. J. Immunol. 165:139. (ELISA)
  6. Chen H, et al. 2005. J. Immunol. 175:591. (ICFC)
  7. Smeltz RB, 2007. J. Immunol. 178:4786. (ICFC)
  8. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (ICFC)
Product Citations
  1. Zhang X, et al. 2017. J Immunol. 10.4049/jimmunol.1602183. PubMed
  2. Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed
  3. Draganov DD, et al. 2019. J Transl Med. 17:100. PubMed
  4. Wei J, et al. 2019. J Immunother Cancer. 7:209. PubMed
  5. Du Q, et al. 2018. J Immunol. 201:533. PubMed
  6. Yang C, et al. 2019. Nat Commun. 10:3931. PubMed
  7. Roybal KT et al. 2016. Cell. 167(2):419-432 . PubMed
  8. Pahl JHW, et al. 2018. Cancer Immunol Res. 0.609027778. PubMed
  9. Boutboul D, et al. 2018. J Clin Invest. 128:3071. PubMed
  10. Ge C, et al. 2020. Cell Reports. 29(13):4236-4244.e3.. PubMed
  11. Evans RDR, et al. 2020. Nat Commun. 3.491666667. PubMed
  12. Norian L, et al. 2009. Cancer Res. 69:3086. PubMed
  13. Longhi M, et al. 2014. PLoS One. 9:87956. PubMed
  14. Goetzmann J, et al. 2014. Proc Natl Acad Sci U S A. 111:8873. PubMed
  15. Tian X, et al. 2015. J Immunol. 194:3873. PubMed
  16. Fang Y, et al. 2021. J Clin Invest. 131:00:00. PubMed
  17. Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed
  18. Pandey A, et al. 2020. Nat Commun. 3.392361111. PubMed
  19. Festag J, et al. 2020. Mol Ther Nucleic Acids. 1.330555556. PubMed
  20. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
  21. Baban B, et al. 2021. JCI Insight. 6:00. PubMed
  22. Cai J, et al. 2021. eLife. 10:00. PubMed
  23. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  24. Wang Y, et al. 2021. Cell Reports. 36(6):109516. PubMed
  25. O'Donoghue GP, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  26. Shao L, et al. 2021. Cell Death Discov. 7:145. PubMed
  27. Devalraju KP, et al. 2021. PLoS One. 16:e0257185. PubMed
  28. Li M, et al. 2021. J Clin Invest. 131:. PubMed
  29. Tesch S, et al. 2020. Sci Rep. 10:21312. PubMed
  30. Prakash MD, et al. 2021. PLoS One. 16:e0247492. PubMed
  31. Guo S, et al. 2021. Stem Cells Int. 2021:9989790. PubMed
  32. Du Y, et al. 2022. Nat Commun. 13:231. PubMed
  33. Anderson AE, et al. 2022. NPJ Regen Med. 7:6. PubMed
  34. Liu W, et al. 2022. Bio Protoc. 12:e4285. PubMed
  35. Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed
  36. Zhou Z, et al. 2022. J Gastrointest Oncol. 13:732. PubMed
  37. Port JR, et al. 2020. J Virol. 94:. PubMed
  38. Shen C, et al. 2021. BMC Med. 19:283. PubMed
  39. Wang H, et al. 2022. Front Immunol. 13:852436. PubMed
  40. Su W, et al. 2022. Front Immunol. 13:952338. PubMed
  41. Cook CP, et al. 2022. Cell Rep Med. 3:100715. PubMed
  42. Harshe RP, et al. 2020. Nat Commun. 11:5894. PubMed
  43. Chandran A, et al. 2022. Cell Rep Med. 3:100557. PubMed
  44. Kim JH, et al. 2020. Sci Rep. 10:1835. PubMed
  45. Swadling L, et al. 2022. Nature. 601:110. PubMed
  46. McMurray JL, et al. 2022. Cell Rep. 39:110858. PubMed
  47. Ogura H, et al. 2022. Nat Commun. 13:7063. PubMed
  48. Peng W, et al. 2023. Cells. 12: . PubMed
  49. Cheng H, et al. 2023. Nat Metab. 5:314. PubMed
  50. Veatch JR, et al. 2022. Cancer Cell. 40:393. PubMed
  51. Tan X, et al. 2023. Adv Sci (Weinh). 10:e2206768. PubMed
RRID
AB_315236 (BioLegend Cat. No. 502511)
AB_315237 (BioLegend Cat. No. 502512)

Antigen Details

Structure
Cytokine; dimer; 20-25 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by vitamin D3 or DMN; labile at pH2
Gene ID
3458 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org
Go To Top Version: 2    Revision Date: 01/07/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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