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APC anti-mouse CD223 (LAG-3) Antibody

Pricing & Availability
Clone
C9B7W (See other available formats)
Regulatory Status
RUO
Other Names
LAG3, LAG-3
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
C9B7W_APC_090310
Con A-stimulated (day-3) C57BL/6 splenocytes stained with C9B7W APC
  • C9B7W_APC_090310
    Con A-stimulated (day-3) C57BL/6 splenocytes stained with C9B7W APC
See APC spectral data
Cat # Size Price Quantity Check Availability Save
125209 25 µg 76€
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125210 100 µg 199€
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Description

CD223, also known as LAG-3, is 70 kD type I transmembrane glycoprotein within the Ig superfamily. It is the gene product of lymphocyte activation gene-3 (Lag3) with structural similarity to CD4. CD223 is expressed on activated T cells and NK cells, as well as T regulatory cells. CD223, like CD4, binds MHC class II molecules, but with higher affinity than CD4. In association with the CD3 complex, CD223 has been shown as a negative regulatory molecule to inhibit TCR-mediated signaling and regulate homeostatic T cell expansion. In contrast to inhibition, CD223 has an activation function to promote immune responses by inducing MHC class II signaling in antigen presenting cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine CD223-Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Workman CJ, et al. 2002. Eur. Immunol. 32:2255. (IP, ELISA)
  2. Workman CJ, et al. 2005. J. Immunol. 174:688. (FC)
  3. Li N, et al. 2004. J. Immunol. 173:6806. (IP, ELISA)
Product Citations
  1. Moore MJ et al. 2018. eLife. 7 pii: e33057. PubMed
  2. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  3. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  4. Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed
  5. Ma X et al. 2019. Cell Metab. 30(1):143-156 . PubMed
  6. Wang J et al. 2018. Cell. 176(1-2):334-347 . PubMed
  7. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  8. DeLong JH, et al. 2019. Immunohorizons. 3:13. PubMed
  9. Hartmann W, et al. 2020. Cell Reports. 29(8):2243-2256.e4.. PubMed
  10. Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed
  11. Miyakoda M, et al. 2012. J Immunol. 189:4396. PubMed
  12. Sande O, et al. 2016. J Immunol. 196: 691 - 702. PubMed
  13. Li M, et al. 2020. J Immunother Cancer. 8:00. PubMed
  14. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
  15. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  16. Dikiy S, et al. 2021. Immunity. 54(5):931-946.e11. PubMed
  17. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  18. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  19. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  20. Ferrere G, et al. 2021. JCI Insight. 6:. PubMed
  21. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  22. Xue G, et al. 2021. Cancer Cell. 39:1610. PubMed
  23. Zeng S, et al. 2023. Front Oncol. 13:1171926. PubMed
RRID
AB_10639935 (BioLegend Cat. No. 125209)
AB_10639727 (BioLegend Cat. No. 125210)

Antigen Details

Structure
Ig superfamily
Distribution

T cells and NK cells, as well as T regulatory cells

Cell Type
NK cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Baixeras E et al. 1992. J. Exp. Med. 176:327.

Gene ID
16768 View all products for this Gene ID
UniProt
View information about CD223 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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