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APC/Cyanine7 anti-human CD20 Antibody

Pricing & Availability
Clone
2H7 (See other available formats)
Regulatory Status
RUO
Workshop
IV B201
Other Names
B1, Bp35
Isotype
Mouse IgG2b, κ
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Product Citations
publications
2H7_APCCy7_120210
Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) APC/Cyanine7 (filled histogram), or mouse IgG2b, κ APC/Cyanine7 (dashed line).
  • 2H7_APCCy7_120210
    Human peripheral blood lymphocytes were stained with anti-CD20 (clone 2H7) APC/Cyanine7 (filled histogram), or mouse IgG2b, κ APC/Cyanine7 (dashed line).
See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
302313 25 tests 115€
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302314 100 tests 234€
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Description

CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human tonsillar B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The epitope recognized by clone 2H7 has been mapped to the sequence YNCEPANPSEKNSPST which lies in the large extracellular loop of human CD20. Additional reported applications (for the relevant formats) include: immunoprecipitation4 and immunohistochemical staining of acetone-fixed frozen sections5.

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.
  4. Polyak MJ, et al. 2002. Blood 99:3256. (IP)
  5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
Product Citations
  1. Garfall AL, et al. 2018. JCI Insight. 3. PubMed
  2. Shehata L, et al. 2019. Nat Commun. 10:1126. PubMed
  3. Fraussen J, et al. 2019. J Immunol. 203:1650. PubMed
  4. Dos Santos JC, et al. 2019. Cell Rep. 28:2659. PubMed
  5. Woodberry T, et al. 2017. Infect Immun. 85:e00986. PubMed
  6. Longbrake EE, et al. 2018. Mult Scler. 24:728. PubMed
  7. Wiedemann A, et al. 2020. Nat Commun. 3.048611111. PubMed
  8. Cirovic B, et al. 2020. Cell Host & Microbe. 28(2):322-334. PubMed
  9. Brocca-Cofano E, et al. 2011. Vaccine. 29:3310. PubMed
  10. Hunt P, et al. 2013. Blood. 121:4653. PubMed
  11. Whittle J, et al. 2014. J Virol. 88:4047. PubMed
  12. Ratliff M, et al. 2015. J Immunol. 194:940. PubMed
  13. Lewis M, et al. 2015. Am J Hum Genet. 96:221. PubMed
  14. Jia M, et al. 2016. J Virol. 90: 4017-4031. PubMed
  15. Juno JA, et al. 2020. Nat Med. 26:1428. PubMed
  16. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  17. Sutton HJ, et al. 2021. Cell Reports. 34(6):108684. PubMed
  18. Bonifacius A, et al. 2021. Immunity. 54(2):340-354.e6. PubMed
  19. Fu Y, et al. 2021. Cell Reports. 36(2):109344. PubMed
  20. Srivastava SK, et al. 2021. Mol Ther Methods Clin Dev. 703:20. PubMed
  21. Nguyen THO, et al. 2021. Immunity. 54:1066. PubMed
  22. Okurut S, et al. 2020. Infect Immun. 88:. PubMed
  23. Wang L, et al. 2021. Science. 373:. PubMed
  24. Verma A, et al. 2021. Cell Rep. 37:109942. PubMed
  25. Palmerin N, et al. 2021. Viruses. 13:. PubMed
  26. Gussarow D, et al. 2021. Front Med (Lausanne). 8:770381. PubMed
  27. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  28. Zou J, et al. 2022. J Clin Invest. 132:. PubMed
  29. Biswas S, et al. 2022. Nat Commun. 13:2995. PubMed
  30. Rabold K, et al. 2022. Nat Commun. 13:6149. PubMed
  31. Alomari N, et al. 2022. Viruses. 14:. PubMed
  32. Liu L, et al. 2022. Sci Transl Med. 14:eabn6859. PubMed
  33. Sefik E, et al. 2022. Nature. 606:585. PubMed
  34. Koutsakos M, et al. 2022. Immunity. 55:1316. PubMed
  35. Chernyshev M, et al. 2023. Nat Commun. 14:2249. PubMed
RRID
AB_314261 (BioLegend Cat. No. 302313)
AB_314262 (BioLegend Cat. No. 302314)

Antigen Details

Structure
Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
Distribution

B cell, T cell subsets

Function
B cell activation
Ligand/Receptor
Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
Cell Type
B cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Hultin L, et al. 1993. Cytometry 14:196.
2. Tedder T, et al. 1994. Immunol. Today 15:450.

Gene ID
931 View all products for this Gene ID
UniProt
View information about CD20 on UniProt.org

Related FAQs

What is the difference between two anti human CD20 clones 2H7 and 1412?

For clone 1412: This clone specifically recognizes cytoplasmic domain of CD20 and thus can only be used for intracellular flow cytometry. In this instance you will need to include the fixation and permeabilization steps. Please follow the intracellular flow cytometry staining protocol.

For clone 2H7: This clone is ok for regular surface staining for CD20 and there is no need for any fixation and permeabilization steps.

Our technical protocols can be found here.

Go To Top Version: 3    Revision Date: 10/08/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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