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APC/Cyanine7 anti-human CD69 Antibody

Pricing & Availability
Clone
FN50 (See other available formats)
Regulatory Status
RUO
Workshop
IV A91
Other Names
Very Early Activation Antigen (VEA), Activation inducer molecule (AIM)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
FN50_APCCyanine7_CD69_Antibody_062119
PMA + Ionomycin stimulated (6 hours) human peripheral blood lymphocytes stained with CD69 (clone FN50) APC/Cyanine7 (filled histogram), or Mouse IgG1, κ APC/Cyanine7 isotype (open histogram).
  • FN50_APCCyanine7_CD69_Antibody_062119
    PMA + Ionomycin stimulated (6 hours) human peripheral blood lymphocytes stained with CD69 (clone FN50) APC/Cyanine7 (filled histogram), or Mouse IgG1, κ APC/Cyanine7 isotype (open histogram).
See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
310913 25 tests 124€
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310914 100 tests 253€
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Description

CD69 is a 27-33 kD type II transmembrane protein also known as activation inducer molecule (AIM), very early activation antigen (VEA), and MLR3. It is a member of the C-type lectin family, expressed as a disulfide-linked homodimer. Other members of this receptor family include NKG2, NKR-P1 CD94, and Ly49. CD69 is transiently expressed on activated leukocytes including T cells, thymocytes, B cells, NK cells, neutrophils, and eosinophils. CD69 is constitutively expressed by a subset of medullary mature thymocytes, platelets, mantle B cells, and certain CD4+ T cells in germinal centers of normal lymph nodes. CD69 is involved in early events of lymphocyte, monocyte, and platelet activation, and has a functional role in redirected lysis mediated by activated NK cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections2, immunofluorescence microscopy3, and spatial biology (IBEX)8,9.

Additional Product Notes

BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp WB, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Sakkas LI, et al. 1998. Clin. and Diag. Lab. Immunol. 5:430. (IHC)
  3. Kim JR, et al. 2005. BMC Immunol. 6:3. (IF)
  4. Verjans GM, et al. 2007. P. Natl. Acad. Sci. USA 104:3496.
  5. Lu H, et al. 2009. Toxicol Sci. 112:363. (FC) PubMed
  6. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  9. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
  2. Schreurs RRCE, et al. 2019. Immunity. 50:462. PubMed
  3. Judge CJ, et al. 2017. J Leukoc Biol. 102:171. PubMed
  4. Di Blasi D, et al. 2019. Cell Mol Gastroenterol Hepatol. 0.510416667. PubMed
  5. Holbrook AK, et al. 2019. Physiol Rep. 7:e14234. PubMed
  6. Lichnog C, et al. 2019. Front Pharmacol. 10:39. PubMed
  7. Pallikkuth S, et al. 2020. eLife. 9:e51889.. PubMed
  8. Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed
  9. Saraiva DP, et al. 2018. Front Immunol. 2.184027778. PubMed
  10. Hughson A, et al. 2011. Diabetes. 60:2125. PubMed
  11. Qi Y, et al. 2012. PLoS One. 7:e39072. PubMed
  12. Koelsch K, et al. 2013. PLoS One. 8:50068. PubMed
  13. Tsai C, et al. 2015. J Immunol. 194:3890. PubMed
  14. Fujigaki J, et al. 2015. PLoS One. 10: 0132521. PubMed
  15. Fu W, et al. 2016. Sci Rep. 6:38162. PubMed
  16. Fisher J, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.03.002. PubMed
  17. Braun J, et al. 2020. Nature. 587:270. PubMed
  18. Sorensen ES, et al. 2020. Antimicrob Agents Chemother. 64:00:00. PubMed
  19. Corgnac S, et al. 2021. STAR Protocols. 2(1):100267. PubMed
  20. Schreurs RRCE, et al. 2021. Mucosal Immunology. 14(3):605-614. PubMed
  21. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  22. Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed
  23. Loo Yau H, et al. 2021. STAR Protocols. 2(2):100549. PubMed
  24. Yin Q, et al. 2021. Proc Natl Acad Sci U S A. 118: . PubMed
  25. Li YR, et al. 2021. Cell Rep Med. 2:100449. PubMed
  26. Arenas EJ, et al. 2021. Nat Commun. 12:1237. PubMed
  27. Paulikat AD, et al. 2022. J Innate Immun. 14:569. PubMed
  28. Corgnac S, et al. 2022. J Immunother Cancer. 10:. PubMed
  29. Cao B, et al. 2022. Nat Commun. 13:6203. PubMed
  30. Zhou Y, et al. 2021. Methods Mol Biol. 2388:35. PubMed
  31. Campisi L, et al. 2022. Nature. 606:945. PubMed
  32. Macedo AB, et al. 2022. J Virol. 96:e0037222. PubMed
  33. Martínez-Sabadell A, et al. 2022. Cell Rep. 41:111430. PubMed
  34. Lin CP, et al. 2023. EMBO J. 42:e111614. PubMed
RRID
AB_314848 (BioLegend Cat. No. 310913)
AB_314849 (BioLegend Cat. No. 310914)

Antigen Details

Structure
C-type lectin, type II glycoprotein, 28/32 kD
Distribution

Activated T cells, B cells, NK cells, granulocytes, thymocytes, platelets, Langerhans cells

Function
Lymphocyte, monocyte, and platelet activation, NK cell killing
Cell Type
B cells, Granulocytes, Langerhans cells, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Testi R, et al. 1994. Immunol. Today 15:479.

Gene ID
969 View all products for this Gene ID
UniProt
View information about CD69 on UniProt.org

Related FAQs

There are no FAQs for this product.
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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