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APC/Cyanine7 anti-mouse NK-1.1 Antibody

Pricing & Availability
Clone
PK136 (See other available formats)
Regulatory Status
RUO
Other Names
NKR-P1C, NKR-P1B, Ly-55, CD161, CD161b, CD161c
Isotype
Mouse IgG2a, κ
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Product Citations
publications
PK136_APCCyanine7_NK11_Antibody_1_022324
C57BL/6 mouse splenocytes were stained with CD49b (clone DX5) FITC and NK1.1 (clone PK136) APC/Cyanine7 (left) or mouse IgG2a, κ APC/Cyanine7 isotype control (right).
  • PK136_APCCyanine7_NK11_Antibody_1_022324
    C57BL/6 mouse splenocytes were stained with CD49b (clone DX5) FITC and NK1.1 (clone PK136) APC/Cyanine7 (left) or mouse IgG2a, κ APC/Cyanine7 isotype control (right).
See APC/Cyanine7 spectral data
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108723 25 µg 76€
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108724 100 µg 234€
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Description

NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK-1+ cells from mouse spleen and bone marrow
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, complement-dependent cytotoxicity3, in vivo depletion4,5,9,10, mediation of in vitro redirected lysis6, blocking of NK cell function7, induction of proliferation8, immunohistochemical staining of frozen sections11, immunofluorescence microscopy11, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 108712).

Additional Product Notes

BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP)
  2. Sentman CL, et al. 1989. Hybridoma 8:605. (IP)
  3. Koo GC, et al. 1984. Hybridoma 3:301. (Cyt)
  4. Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete)
  5. Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete)
  6. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  7. Kung SK, et al. 1999. J. Immunol. 162:5876. (Block)
  8. Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143.
  9. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  10. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete)
  11. Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF)
  12. Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed
  13. Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed
  14. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  15. Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  2. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  3. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  4. Lee SC et al. 2018. Cancer cell. 34(2):225-241 . PubMed
  5. Seo GY et al. 2018. Cell host & microbe. 24(2):249-260 . PubMed
  6. Djokić V, et al. 2019. Front Immunol. 9:2891. PubMed
  7. Kiyohara H, et al. 2018. Cell Mol Gastroenterol Hepatol. 7:135. PubMed
  8. Barth ND, et al. 2018. J Immunol. 201:2602. PubMed
  9. Xu W et al. 2019. Immunity. 50(4):1054-1068 . PubMed
  10. Goossens P, et al. 2019. Cell Metab. 29:1376. PubMed
  11. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  12. Zhou J, et al. 2019. Immunity. 50:403. PubMed
  13. Rohner L, et al. 2020. Sci Rep. 0.570833333. PubMed
  14. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  15. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  16. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  17. Etzerodt A, et al. 2019. J Exp Med. 216:2394. PubMed
  18. Li J, et al. 2018. Immunity. 49:178. PubMed
  19. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  20. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  21. Brodin P, et al. 2010. PLoS One. 5:e13174. PubMed
  22. Mitchell A, et al. 2012. J Immunol. 189:4970. PubMed
  23. Bunn P, et al. 2014. J Immunol. 192:3709. PubMed
  24. Chung Y, et al. 2014. J Vis Exp. 89: 51660. PubMed
  25. Montes de Oca M, et al. 2016. PLoS Pathog. 12: 1005398. PubMed
  26. Lee L, et al. 2016. PLoS One. 11:e0167693. PubMed
  27. Etzerodt A, et al. 2020. J Exp Med. 217:00:00. PubMed
  28. Aldrich AL, et al. 2021. The Journal of Immunology. 206(4):751-765. PubMed
  29. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  30. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  31. Desai P, et al. 2021. Cell. 184(5):1214-1231.e16. PubMed
  32. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  33. Mulas F, et al. 2020. Cell Mol Immunol. . PubMed
  34. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  35. DiToro D, et al. 2020. Immunity. 650:52. PubMed
  36. Dos Santos Dias L, et al. 2021. PLoS Pathog. e1009324:17. PubMed
  37. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
  38. Henrich IC, et al. 2021. Cancer Res. 81:2171. PubMed
  39. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
  40. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  41. Akoolo L, et al. 2021. Cell Microbiol. 23:e13350. PubMed
  42. De Veirman K, et al. 2021. Oncoimmunology. 10:2000699. PubMed
  43. Katzmarski N, et al. 2021. Nat Immunol. 22:1382. PubMed
  44. Lee S, et al. 2021. Autophagy. 18:1062. PubMed
  45. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  46. Grune J, et al. 2022. Nat Cardiovasc Res. 1:649. PubMed
  47. Qiao Y, et al. 2021. Oncoimmunology. 10:1947569. PubMed
  48. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  49. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
  50. Lingegowda H, et al. 2022. Front Reprod Health. 3:726936. PubMed
  51. Silva Pereira S, et al. 2022. Elife. 11: . PubMed
  52. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  53. Choi H, et al. 2023. J Nanobiotechnology. 21:5. PubMed
  54. Agrafiotis A, et al. 2023. iScience. 26:106055. PubMed
  55. Giampaolo S, et al. 2023. iScience. 26:106234. PubMed
RRID
AB_830870 (BioLegend Cat. No. 108723)
AB_830871 (BioLegend Cat. No. 108724)

Antigen Details

Structure
NKR-P1 gene family
Distribution

NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)

Function
NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.

Gene ID
17059 View all products for this Gene ID
UniProt
View information about NK-1.1 on UniProt.org
Go To Top Version: 2    Revision Date: 12/08/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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