Loading...

Biotin anti-mouse CD4 Antibody

Pricing & Availability
Clone
GK1.5 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
GK1dot5_Biotin_011108
C57BL/6 mouse splenocytes were stained with biotinylated CD4 (clone GK1.5) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by Sav-PE.
  • GK1dot5_Biotin_011108
    C57BL/6 mouse splenocytes were stained with biotinylated CD4 (clone GK1.5) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by Sav-PE.
Cat # Size Price Quantity Check Availability Save
100403 50 µg 29€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
100404 500 µg 92€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CTL clone V4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1,4,11, thymocyte costimulation3, in vitro and in vivo depletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, immunoprecipitation1,2, and spatial biology (IBEX)12,13. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Dialynas DP, et al. 1983. J. Immunol. 131:2445. (Block, IP)
  2. Dialynas DP, et al. 1983. Immunol. Rev. 74:29. (IP, Deplete)
  3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim)
  4. Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block)
  5. Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete)
  6. Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete)
  7. Ghobrial RR, et al. 1989. Clin. Immunol. Immunopathol. 52:486. (Deplete)
  8. Israelski DM, et al. 1989. J. Immunol. 142:954. (Deplete)
  9. Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC)
  10. Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC)
  11. Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block)
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  2. Sevin M, et al. 2018. Nat Commun. 9:1431. PubMed
  3. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  4. Wang Z et al. 2018. Immunity. 49(1):80-92 . PubMed
  5. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  6. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  7. Zhang Z, et al. 2019. Haematologica. 4.339583333. PubMed
  8. Matsumura T et al. 2019. Cell Rep. 27(2):561-571 . PubMed
  9. Cheng HW, et al. 2019. Nat Commun. 10:1739. PubMed
  10. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  11. Zhu J et al. 2017. Cell stem cell. 20(3):374-384 . PubMed
  12. Miyazaki M et al. 2017. Immunity. 46(5):818-834 . PubMed
  13. Bian Y, et al. 2017. PLoS Pathog. 13:e1006384. PubMed
  14. Lin H, et al. 2019. Infect Immun. 87:e00055-19. PubMed
  15. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  16. Khaw YM, et al. 2020. J Neuroinflammation. 17:49. PubMed
  17. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  18. Liang J, et al. 2017. Nat Commun. 8:15732. PubMed
  19. Au-Yeung BB, et al. 2017. J Immunol. 198:2445. PubMed
  20. Freise AC, et al. 2017. Mol Imaging Biol. 19:599. PubMed
  21. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  22. Ji Y, et al. 2019. Nat Immunol. 1.303472222. PubMed
  23. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
  24. He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
  25. Poholek CH, et al. 2019. Immunohorizons. 0.259722222. PubMed
  26. Inamine A, et al. 2012. Clin Immunol. 143:170. PubMed
  27. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  28. Ho K, et al. 2014. Cell Death Dis. 5:1518. PubMed
  29. Boulay A, et al. 2015. J Neurosci. 35:4427. PubMed
  30. Muro R, et al. 2015. PLoS One. 10:119898. PubMed
  31. Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed
  32. Van de Velde L, et al. 2016. Cell Rep. 17:2247-2258. PubMed
  33. Han X, et al. 2017. Int J Mol Sci. 10.3390/ijms18050942. PubMed
  34. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  35. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  36. Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed
  37. Milner JJ, et al. 2020. Immunity. 52(5):808-824.e7. PubMed
  38. Opejin A, et al. 2020. Cell Reports. 33(8):108424. PubMed
  39. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  40. He Y, et al. 2021. Cell Metabolism. 33(5):988-1000.e7. PubMed
  41. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  42. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  43. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
  44. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  45. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  46. Xie L, et al. 2020. Infect Immun. :88. PubMed
  47. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  48. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  49. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  50. Dong S, et al. 2021. Nature. 591:117. PubMed
  51. Aftabizadeh M, et al. 2021. JCI Insight. 6:. PubMed
  52. Amend A, et al. 2021. Int J Mol Sci. 22:. PubMed
  53. Onodera T, et al. 2021. Immunity. 54:2385. PubMed
  54. Suah AN, et al. 2021. J Clin Invest. 131:. PubMed
  55. Zhai X, et al. 2021. Sci Adv. 7:eabk0490. PubMed
  56. Koide S, et al. 2022. iScience. 25:103603. PubMed
  57. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  58. Schönberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
  59. She L, et al. 2021. JCI Insight. 6:e143509. PubMed
  60. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  61. Zhou Z, et al. 2022. Infect Immun. 90:e0045321. PubMed
  62. Waldman MM, et al. 2022. Front Immunol. 13:856977. PubMed
  63. Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
  64. Tian Q, et al. 2021. Infect Immun. 89:e0020521. PubMed
  65. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  66. Florentin J, et al. 2022. Front Immunol. 13:882484. PubMed
  67. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  68. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  69. Chanda MK, et al. 2022. J Immunol Methods. 506:113290. PubMed
  70. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  71. Kraemer AN, et al. 2022. Front Immunol. 13:933191. PubMed
  72. Horkova V, et al. 2023. Nat Immunol. 24:174. PubMed
  73. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  74. Tanaka T, et al. 2023. Nat Immunol. 24:439. PubMed
  75. Yeh CH, et al. 2022. Immunity. 55:272. PubMed
  76. De Giovanni M, et al. 2022. Cell. 185:815. PubMed
  77. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  78. Miyake K, et al. 2023. Nat Commun. 14:2694. PubMed
RRID
AB_312688 (BioLegend Cat. No. 100403)
AB_312689 (BioLegend Cat. No. 100404)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
Go To Top Version: 1    Revision Date: 11/30/2012

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account