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Brilliant Violet 605™ anti-human CD8a Antibody

Pricing & Availability
Clone
RPA-T8 (See other available formats)
Regulatory Status
RUO
Workshop
IV T171
Other Names
T8, Leu2
Isotype
Mouse IgG1, κ
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Product Citations
publications
RPA-T8_BV605_013112
Human peripheral blood lymphocytes were stained with CD3 FITC and CD8 (clone RPA-T8) Brilliant Violet 605™.
  • RPA-T8_BV605_013112
    Human peripheral blood lymphocytes were stained with CD3 FITC and CD8 (clone RPA-T8) Brilliant Violet 605™.
See Brilliant Violet 605™ spectral data
Cat # Size Price Quantity Check Availability Save
301039 25 tests 176€
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301040 100 tests 335€
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Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The RPA-T8 antibody does not block the binding of HIT8a antibody to CD8a. Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of paraformaldehyde-fixed frozen sections3 and costimulation of T cell responses4. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 301073 & 301074).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  3. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  4. Magidovich E, et al. 2007. P. Natl. Acad. Sci. USA 104:13022.
  5. Thakarl D, et al. 2008.J. immunol. 180:7431. PubMed
  6. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  7. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  10. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
Product Citations
  1. Johnson L, Olson B, and McNeel D. 2017. J Immunother Cancer. 10.1186/s40425-017-0260-3. PubMed
  2. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  3. Lindestam Arlehamn CS, et al. 2019. J Immunol. 203:84. PubMed
  4. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  5. Johnson LE, et al. 2017. J Immunother Cancer. 5:56. PubMed
  6. Charpentier JC, et al. 2020. Nat Commun. 11:180. PubMed
  7. Claiborne DT, et al. 2019. PLoS Pathog. 15:e1007981. PubMed
  8. Carter JA, et al. 2020. Cell Systems. 9(5):475-482.e4.. PubMed
  9. Claiborne D, et al. 2015. Proc Natl Acad Sci U S A. 112:1480. PubMed
  10. Kuebler P, et al. 2015. Proc Natl Acad Sci U S A. 112: 8379 - 8384. PubMed
  11. Pombo C, et al. 2015. J Infect Dis. 12: 1376-1386. PubMed
  12. Schommers P, et al. 2016. J Virol. 90: 7579 - 7586. PubMed
  13. Briceño O, et al. 2016. PLoS One. 11:e0166496. PubMed
  14. Tauriainen J, et al. 2017. Sci Rep. 7:40354. PubMed
  15. Pinto-Cardoso S, et al. 2017. Sci Rep. 10.1038/srep43741. PubMed
  16. Woldemeskel BA, et al. 2020. J Clin Invest. 130:6631. PubMed
  17. Buggert M, et al. 2020. Cell. 183(7):1946-1961.e15. PubMed
  18. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  19. Dupraz L, et al. 2021. Cell Reports. 36(1):109332. PubMed
  20. Argüello RJ, et al. 2020. Cell Metab. 32:1063. PubMed
  21. Yin W, et al. 2021. Thorac Cancer. 12:2680. PubMed
  22. Cheng L, et al. 2021. Cancer Immunol Immunother. Online ahead of print. PubMed
  23. Yarmarkovich M, et al. 2021. Nature. 599:477. PubMed
  24. Jiguet-Jiglaire C, et al. 2022. Acta Neuropathol Commun. 10:1. PubMed
  25. Georg P, et al. 2022. Cell. 185:493. PubMed
  26. Sung BY, et al. 2022. J Clin Invest. 132:. PubMed
  27. Woldemeskel BA, et al. 2022. J Clin Invest. 132:. PubMed
  28. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  29. Dean JW, et al. 2020. J Autoimmun. 108:102417. PubMed
  30. Zeng N, et al. 2022. EMBO Rep. 23:e53302. PubMed
  31. Naaber P, et al. 2022. Cell Rep Med. 3:100716. PubMed
  32. Stevenson EM, et al. 2022. Nat Commun. 13:4888. PubMed
  33. Mujal AM, et al. 2022. Cancer Immunol Res. 10:403. PubMed
  34. Combes AJ, et al. 2022. Cell. 185:184. PubMed
  35. Gartshteyn Y, et al. 2023. Life Sci Alliance. 6: . PubMed
RRID
AB_11126985 (BioLegend Cat. No. 301039)
AB_2563185 (BioLegend Cat. No. 301040)

Antigen Details

Structure
Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
Distribution

Majority of thymocytes, T cell subset, NK cells

Function
MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor
MHC Class I molecules
Cell Type
Dendritic cells, NK cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
925 View all products for this Gene ID
UniProt
View information about CD8alpha on UniProt.org
Go To Top Version: 3    Revision Date: 07/13/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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