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Brilliant Violet 785™ anti-human CD279 (PD-1) Antibody

Pricing & Availability
Clone
EH12.2H7 (See other available formats)
Regulatory Status
RUO
Other Names
PD-1, PDCD1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
EH12.2H7_BV785_061412
Human peripheral blood lymphocytes were stained with CD3 FITC and CD279 (clone EH12.2H7) Brilliant Violet 785™.
  • EH12.2H7_BV785_061412
    Human peripheral blood lymphocytes were stained with CD3 FITC and CD279 (clone EH12.2H7) Brilliant Violet 785™.
See Brilliant Violet 785™ spectral data
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329929 25 tests 190€
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329930 100 tests 357€
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Description

Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1) and PD-L2 (B7-DC) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of ligand binding1-3, immunohistochemical staining of paraformaldehyde fixed frozen sections13, and spatial biology (IBEX)15,16. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329911 and 329912). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 329926) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Dorfman DM, et al. 2006 Am. J. Surg. Pathol. 30:802. (FA)
  2. Radziewicz H, et al. 2007. J. Virol. 81:2545. (FA)
  3. Velu V, et al. 2007. J. Virol. 81:5819. (FA)
  4. Zahn RC, et al. 2008. J. Virol. 82:11577. PubMed
  5. Chang WS, et al. 2008. J. Immunol. 181:6707. (FC) PubMed
  6. Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (FA)
  7. Jones RB, et al. 2009. J. Virol. 83:8722. (FC) PubMed
  8. Vojnov L, et al. 2010. J. Virol. 84:753. (FC) PubMed
  9. Radziewicz H, et al. 2010. J. Immunol. 184:2410. (FC) PubMed
  10. Monteriro P, et al. 2011. J. Immunol. 186:4618. PubMed
  11. Conrad J, et al. 2011. J. Immunol. 186:6871. PubMed
  12. Salisch NC, et al. 2010. J. Immunol. 184:476. (Rhesus reactivity)
  13. Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
  14. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  15. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  16. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Tardif V, et al. 2019. Nat Commun. 10:823. PubMed
  2. Pauthner MG, et al. 2019. Immunity. 50:241. PubMed
  3. Cirelli KM et al. 2019. Cell. 177(5):1153-1171 . PubMed
  4. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  5. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  6. Schreurs RRCE, et al. 2019. Immunity. 50:462. PubMed
  7. Di Blasi D, et al. 2019. Cell Mol Gastroenterol Hepatol. 0.510416667. PubMed
  8. Nishimura Y, et al. 2017. Nature. 543:559. PubMed
  9. Colomb F, et al. 2020. Cell Reports. 32(5):107991. PubMed
  10. Herati R, et al. 2014. J Immunol. 193:3528. PubMed
  11. Dan J, et al. 2016. J Immunol. 197: 983 - 993. PubMed
  12. Tauriainen J, et al. 2017. Sci Rep. 7:40354. PubMed
  13. Hoang TN, et al. 2021. Cell. 184:460. PubMed
  14. Rydyznski Moderbacher C, et al. 2020. Cell. 183(4):996-1012.e19. PubMed
  15. Harper JL, et al. 2020. Nat Med. 519:26. PubMed
  16. Hoang TN, et al. 2020. bioRxiv. . PubMed
  17. Yu C, et al. 2021. Med (N Y). 2:755. PubMed
  18. Vardam-Kaur T, et al. 2021. Oncotarget. 12:2051. PubMed
  19. Zhang P, et al. 2021. Nat Med. 22:. PubMed
  20. Lozano-Rodríguez R, et al. 2022. Cell Rep. 38:110235. PubMed
  21. Silva M, et al. 2021. Sci Immunol. 6:eabf1152. PubMed
  22. Ireland RE, et al. 2022. Viruses. 14:. PubMed
  23. Rydyznski Moderbacher C, et al. 2022. J Clin Invest. :. PubMed
  24. Gyurdieva A, et al. 2022. Nat Commun. 13:5296. PubMed
  25. Panigrahi S, et al. 2020. Eur J Immunol. 50:2055. PubMed
  26. Lorvik KB, et al. 2021. Front Immunol. 12:744155. PubMed
  27. Sudmeier LJ, et al. 2022. Cell Rep Med. 3:100620. PubMed
  28. McEvoy CM, et al. 2022. Nat Commun. 13:7634. PubMed
  29. Xu Q, et al. 2023. Nat Immunol. 24:186. PubMed
  30. Preglej T, et al. 2023. Front Immunol. 14:1096096. PubMed
  31. Doloff JC, et al. 2023. Sci Adv. 9:eade9488. PubMed
RRID
AB_11218984 (BioLegend Cat. No. 329929)
AB_2563443 (BioLegend Cat. No. 329930)

Antigen Details

Structure
Immunoglobulin superfamily
Distribution

Transiently expressed on CD4- CD8- thymocytes; upregulated in thymocytes and splenic T and B lymphocytes; expressed on activated myeloid cells

Ligand/Receptor
B7-H1 (also known as PD-L1) and B7-DC (PD-L2)
Cell Type
B cells, Lymphocytes, T cells, Thymocytes, Tregs
Biology Area
Cancer Biomarkers, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Gene ID
5133 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org
Go To Top Version: 2    Revision Date: 01/22/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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