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PE anti-human CD25 Antibody

Pricing & Availability
Clone
M-A251 (See other available formats)
Regulatory Status
RUO
Workshop
IV A053
Other Names
IL-2 receptor α chain, Low affinity IL-2R, IL-2Rα chain
Isotype
Mouse IgG1, κ
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Product Citations
publications
M-A251_PE_CD25_Antibody_FC_021113
PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone M-A251) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • M-A251_PE_CD25_Antibody_FC_021113
    PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone M-A251) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
See PE spectral data
Cat # Size Price Quantity Check Availability Save
356103 25 tests 66€
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356104 100 tests 144€
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356134 100 µg 147€
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Description

CD25 is a 55 kD type I transmembrane glycoprotein also known as low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. Soluble CD25/IL-2Rα is produced as a consequence of lymphocyte stimulation and is found in biological fluids following inflammatory responses. CD25 associates with IL-2 receptor β (CD122) and common γ (CD132) chains to form a high affinity IL-2R complex.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PHA-induced lymphocyte cells
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.4 µg per million cells in 100 µl volume. For flow cytometric staining using tests sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde fixed frozen sections1 and spatial biology (IBEX)2,3.

The CD25 molecule reveals three epitope regions: A, B, and C. M-A251 antibody recognizes epitope region B. Unlike other CD25 antibody clones, M-A251 can detect CD25 after fixation with paraformaldehyde.

Application References

(PubMed link indicates BioLegend citation)
  1. Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
  2. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  3. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Maritz MF, et al. 2018. PLoS One. 13:e0196043. PubMed
  2. Cignarella F et al. 2018. Cell metabolism. 27(6):1222-1235 . PubMed
  3. Serra–Peinado C, et al. 2019. Nat Commun. 10:3705. PubMed
  4. Abd Hamid M et al. 2019. Cancer Immunol Res. 7(8):1293-1306 . PubMed
  5. Woodberry T, et al. 2017. Infect Immun. 85:e00986. PubMed
  6. Bassaganyas L, et al. 2019. J Cell Biol. 218:3861. PubMed
  7. Holbrook AK, et al. 2019. Physiol Rep. 7:e14234. PubMed
  8. Zhou Y, et al. 2017. Front Cell Infect Microbiol. 7:457. PubMed
  9. Metz PJ, et al. 2020. Cell Reports. 30(6):1935-1950. PubMed
  10. Tai YT, et al. 2019. Leukemia. 33:426. PubMed
  11. Waddington KE, et al. 2020. Front Immunol. 1.51875. PubMed
  12. Hoseini SS, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(5):. PubMed
  13. Yang YK, et al. 2021. Stem Cell Res Ther. 12:156. PubMed
  14. Lake CM, et al. 2021. Cell Death Dis. 12:400. PubMed
  15. Berezhnoy A, et al. 2020. Cell Rep Med. 1:100163. PubMed
  16. Gamradt S, et al. 2021. iScience. 24:103312. PubMed
  17. Schmitz T, et al. 2021. J Reprod Immunol. 148:103424. PubMed
  18. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
  19. Bossini-Castillo L, et al. 2022. Cell Genom. 2:. PubMed
  20. Ackerley CG, et al. 2022. Front Immunol. 13:972170. PubMed
  21. Chauvin C, et al. 2023. iScience. 26:106124. PubMed
  22. Parasar P, et al. 2022. Am J Reprod Immunol. 88:e13614. PubMed
  23. Ke F, et al. 2023. Elife. 12:. PubMed
  24. Deering RP, et al. 2023. Sci Rep. 13:8452. PubMed
RRID
AB_2561860 (BioLegend Cat. No. 356103)
AB_2561861 (BioLegend Cat. No. 356104)
AB_2564145 (BioLegend Cat. No. 356134)

Antigen Details

Structure
Type I transmembrane glycoprotein, 55 kD; low-affinity IL-2 receptor α chain
Distribution

Activated T and B cells, monocytes/macrophages, Tregs

Interaction
Associates with IL-2Rβ/CD122 and IL-2Rγ/CD132 receptor chains to form a high-affinity IL-2R complex
Ligand/Receptor
IL-2
Cell Type
B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
2. Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
3. Barclay N, et al. 1997. The Leukocyte Antigen FactsBook. Academic Press Inc.
4. Taniguchi T and Minami Y. et al. 1993. Cell 73:5.
5. Waldmann T. 1991. J. Biol. Chem. 266:2681.

Gene ID
3559 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 4    Revision Date: 01/22/2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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