Loading...

PE anti-human CD45RO Antibody

Pricing & Availability
Clone
UCHL1 (See other available formats)
Regulatory Status
RUO
Workshop
IV N31
Other Names
CD45RO
Isotype
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
UCHL1
Human peripheral blood lymphocytes stained with UCHL1 PE
  • UCHL1
    Human peripheral blood lymphocytes stained with UCHL1 PE
  • UCHL1_PE_072909.jpg
    Human peripheral blood lymphocytes stained with UCHL1 PE and CD45RA (HI100) Alexa Fluor® 647
See PE spectral data
Cat # Size Price Quantity Check Availability Save
304205 25 tests 40€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
304206 100 tests 88€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
304244 100 µg 94€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD45RO is a 180 kD single chain membrane glycoprotein. It is a splice variant of tyrosine phosphatase CD45, lacking the A, B, and C determinants. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1 and CD22. CD45 isoform expression can change in response to cytokines.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
IL-2 dependent T cell line, CA1
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.2 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The UCHL1 antibody is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5 and formalin-fixed paraffin-embedded tissue sections4, Western blotting2, and immunoprecipitation3.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (FC)
  2. Ishii T, et al. 2001. P. Natl. Acad. Sci. USA 98:12138. (WB)
  3. Ponsford M, et al. 2001. Clin. Exp. Immunol. 124:315. (IP)
  4. Yamada M, et al. 1996. Stroke 27:1155. (IHC)
  5. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immunol. 5:430. (IHC)
  6. Baba N, et al. 2010. Int. Immunol. 22:237. PubMed
  7. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  8. Weiss L, et al. 2010. P. Natl. Acad. Sci. USA 107:10632. PubMed
  9. Wu YY, et al. 2007. Infect. Immun. 75:4357. PubMed
  10. Mozaffarian N, et al. 2008. Rheumatology 47:1335. PubMed
  11. Roque S, et al. 2007. J. Immunol. 178:8028. PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Smith SH, et al. 1986. Immunology 58:63. (Immunogen)
  14. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Carisey AF, et al. 2018. Curr Biol. 28:489. PubMed
  2. Murakami T, et al. 2018. Nat Commun. 9:2436. PubMed
  3. Godbersen C, et al. 2017. Mol Cancer Ther. 16:1335. PubMed
  4. Shan L, et al. 2017. Immunity. 47:766. PubMed
  5. Evans RDR, et al. 2020. Nat Commun. 3.491666667. PubMed
  6. Zhao B, et al. 2013. PLoS One. 8:77708. PubMed
  7. Sutavani R, et al. 2013. J Immunol. 191:5895. PubMed
  8. Iannetta M, et al. 2016. PLoS One. 11: 0160277. PubMed
  9. Narsale A, Moya R, Robertson H 2016. Data Brief. 8: 1348-51. PubMed
  10. Islas-Vazquez L, et al. 2020. Biology (Basel). 9:00. PubMed
  11. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  12. Li M, et al. 2020. Nat Commun. 4051:11. PubMed
  13. Lee YJ, et al. 2021. Front Immunol. 615369:12. PubMed
  14. Argüello RJ, et al. 2020. Cell Metab. 32:1063. PubMed
  15. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  16. Li M, et al. 2021. J Clin Invest. 131:. PubMed
  17. Chang MH, et al. 2021. Cell Rep. 37:109902. PubMed
  18. Wu D, et al. 2020. Biomark Res. 8:3. PubMed
  19. Hirama T, et al. 2021. JCI Insight. 6:. PubMed
  20. Wang Z, et al. 2021. Cell Mol Immunol. 18:2188. PubMed
  21. Guo J, et al. 2022. Front Cell Dev Biol. 9:775599. PubMed
  22. Zhou Z, et al. 2022. J Gastrointest Oncol. 13:732. PubMed
  23. Jarosch S, et al. 2022. STAR Protoc. 3:101374. PubMed
  24. Elston L, et al. 2020. Br J Haematol. 188:872. PubMed
  25. Wu K, et al. 2021. Aging (Albany NY). 13:. PubMed
  26. Jarosch S, et al. 2021. Cell Rep Methods. 1:100104. PubMed
  27. Li M, et al. 2023. Front Immunol. 14:1087923. PubMed
  28. Si W, et al. 2023. iScience. 26:106529. PubMed
  29. Crawford MP, et al. 2023. PLoS One. 18:e0285166. PubMed
RRID
AB_314421 (BioLegend Cat. No. 304205)
AB_314422 (BioLegend Cat. No. 304206)
AB_2564160 (BioLegend Cat. No. 304244)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane, 180 kD (isoform of CD45 containing none of the A, B, or C determinants)
Distribution

Activated and memory T cells, B cell subsets, monocytes, macrophages, granulocytes

Function
Enhances TCR and BCR signaling
Ligand/Receptor
CD22
Cell Type
B cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.

Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45RO on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 07/23/2013

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account