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PE anti-human CD56 (NCAM) Antibody

Pricing & Availability
Clone
MEM-188 (See other available formats)
Regulatory Status
RUO
Workshop
VI NK26
Other Names
NCAM, Leu-19, NKH1
Isotype
Mouse IgG2a, κ
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Product Citations
publications
MEM-188_PE_070207
Human peripheral blood lymphocytes stained with MEM-188 PE
  • MEM-188_PE_070207
    Human peripheral blood lymphocytes stained with MEM-188 PE
See PE spectral data
Cat # Size Price Quantity Check Availability Save
304605 25 tests 98€
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304606 100 tests 215€
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Description

CD56 is a single transmembrane glycoprotein also known as N-CAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KG-1 human acute myelogenous leukemia cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections, and Western blotting (non-reducing).

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
Product Citations
  1. Ding S, et al. 2018. Sci Rep. 8:10808. PubMed
  2. Dulberger CL et al. 2017. Immunity. 46(6):1018-1029 . PubMed
  3. Dothel G, et al. 2019. World J Gastroenterol. 25:4892. PubMed
  4. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  5. Chen Q, et al. 2009. Proc Natl Acad Sci U S A. 106:21783. PubMed
  6. Zaniboni A, et al. 2014. Am J Physiol Cell Physiol. 306:322. PubMed
  7. Collison J, et al. 2015. J Immunol. 195: 1162-1170. PubMed
  8. Zaniboni A, et al. 2015. Am J Physiol Cell Physiol. 309: C320-C331. PubMed
  9. Kariminia A, et al. 2016. Blood. 127: 3082 - 3091. PubMed
  10. Li M, et al. 2020. Virol Sin. 35:588. PubMed
  11. Sweeney EE, et al. 2020. Nano Res. 13:736. PubMed
  12. Liu Z, et al. 2021. Cells. 10:. PubMed
  13. Manukyan G, et al. 2020. Auto Immun Highlights. 11:5. PubMed
  14. Guo S, et al. 2021. Stem Cells Int. 2021:9989790. PubMed
  15. Koui Y, et al. 2021. Stem Cell Reports. 16:3050. PubMed
  16. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  17. Rosa TLSA, et al. 2022. Front Med (Lausanne). 9:899998. PubMed
  18. Luo H, et al. 2023. Bio Protoc. 13:e4693. PubMed
RRID
AB_314447 (BioLegend Cat. No. 304605)
AB_314448 (BioLegend Cat. No. 304606)

Antigen Details

Structure
Ig superfamily, single transmembrane or GPI-anchored glycoprotein, 175-185 kD
Distribution

NK cells, T subset, neural tissue, some LGL and myeloid leukemias

Function
Adhesion
Ligand/Receptor
Heparin sulfate
Cell Type
Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Lanier L, et al. 1991. J. Immunol. 146:4421.
2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71.
3. Cremer H, et al. 1994. Nature 367:455.

Gene ID
4684 View all products for this Gene ID
UniProt
View information about CD56 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 12/16/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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