PE anti-human CD83 Antibody

Product Details

Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections⁴.

Application References

(PubMed link indicates BioLegend citation)

1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press New York.
2. Zhou L, et al. 1995. J. Immunol. 154:3821.
3. Cao W, et al. 2005. Biochem. J. 385:85.
4. Lore K, et al. 2002. AIDS 16:683. (IHC)
5. Cho H, et al. 2007. Physiol Genomics doi:10.1152/physiolgenomics.00051.2006

Product Citations

1. Murakami T, et al. 2018. Nat Commun. 9:2436. PubMed
2. Zhang W, et al. 2019. Mar Drugs. 0.754166667. PubMed
3. Martín–Gayo E, et al. 2020. Cell Rep. 30:984. PubMed
4. Blengio F, et al. 2013. Immunobiology. 218:76. PubMed
5. Škrnjug I, et al. 2014. PLoS One. 9:95728. PubMed
6. Beljanski V, et al. 2015. J Virol. 89: 10612 - 10624. PubMed
7. Eriksson E, et al. 2016. Gene Ther. 10.1038/gt.2016.80. PubMed
8. Sutton HJ, et al. 2021. Cell Reports. 34(6):108684. PubMed
9. Kan S, et al. 2020. Int J Oncol. 57:1047. PubMed
10. Chen R, et al. 2021. Front Oncol. 11:743050. PubMed
11. Fitzgerald KC, et al. 2021. Cell Rep Med. 2:100424. PubMed
12. Nazitto R, et al. 2021. J Immunol. 206:2949. PubMed
13. Stich L, et al. 2022. Int J Mol Sci. 23:. PubMed
14. Shen Z, et al. 2022. EBioMedicine. 85:104285. PubMed
15. Perera N, et al. 2022. Antiviral Res. 199:105269. PubMed
16. Solier S, et al. 2023. Nature. 617:386. PubMed

RRID

AB_314515 (BioLegend Cat. No. 305307)
AB_314516 (BioLegend Cat. No. 305308)
AB_2076529 (BioLegend Cat. No. 305322)

Antigen Details

Structure

Ig superfamily, single chain transmembrane glycoprotein, 43 kD

Distribution

Dendritic cells, Langerhan cells, activated B and T cells

Cell Type

B cells, Dendritic cells, Langerhans cells, T cells

Biology Area

Costimulatory Molecules, Immunology

Molecular Family

CD Molecules

Antigen References

1. Kozlow E, et al. 1993. Blood 81:454.
2. Zhou L, et al. 1992. J. Immunol. 149:735.
3. Zhou L, et al. 1995. Blood 86:3295.

Gene ID

9308 View all products for this Gene ID

UniProt

View information about CD83 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

What type of PE do you use in your conjugates?

We use R-PE in our conjugates.

Other Formats

Concentration & Expiration Lookup

Certificate of Analysis