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PE anti-human HLA-A2 Antibody

Pricing & Availability
Clone
BB7.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC-class I
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A_BB7dot2_PE_100809
HLA-A2 positive (top) and HLA-A2 negative (bottom) donors peripheral blood lymphocytes stained with BB7.2 PE.
  • A_BB7dot2_PE_100809
    HLA-A2 positive (top) and HLA-A2 negative (bottom) donors peripheral blood lymphocytes stained with BB7.2 PE.
  • B_BB7dot2_PE_100809
See PE spectral data
Cat # Size Price Quantity Check Availability Save
343305 25 tests 104€
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343306 100 tests 227€
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Description

HLA-A2 is most common in Northern Asia and North America populations. MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8+ T cells, playing an important role in cell-mediated immune responses and tumor surveillance.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Papain solubilized HLA-A2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The BB7.2 antibody recognizes human leukocyte antigen (HLA) A2 which is a subset of MHC-class I molecules encoded by A*02 alleles.

Additional reported applications (for the relevant formats) include: immunoprecipitation3.

Application References

(PubMed link indicates BioLegend citation)
  1. Brodsky FM, et al. 1979. Immunol. Rev. 47:3.
  2. Parham P and Brodsky FM. et al. 1981. Hum. Immunol. 3:277.
  3. Lubben NB, et al. 2007. Mol Biol Cell. 18:3351. (IP)
Product Citations
  1. Gu C, et al. 2019. J Immunol. 203:389. PubMed
  2. Gärtner K, et al. 2019. J Extracell Vesicles. 8:1573051. PubMed
  3. Barili V, et al. 2020. Nat Commun. 0.877777778. PubMed
  4. Abdelaziz MO, et al. 2019. Front Immunol. 1.65. PubMed
  5. Wang X, et al. 2011. Blood. 117:1888. PubMed
  6. Schuler P, et al. 2014. Clin Cancer Res. 20:2433. PubMed
  7. Ameres S, et al. 2014. J Immunol. 192:5894. PubMed
  8. Lai J, et al. 2016. Blood. 128: 1396 - 1407. PubMed
  9. Pettmann J, et al. 2021. eLife. 10:00. PubMed
  10. Bagati A, et al. 2020. Cancer Cell. 39(1):54-67.e9. PubMed
  11. Melacarne A, et al. 2021. Cell Reports. 36(1):109312. PubMed
  12. Pellegrini JM, et al. 2021. Sci Rep. 11:13559. PubMed
  13. FitzPatrick MEB, et al. 2021. Cell Rep. 34:108661. PubMed
  14. Klatt MG, et al. 2020. J Proteomics. 228:103921. PubMed
  15. Qiu C, et al. 2022. Front Immunol. 12:764949. PubMed
  16. Deng J, et al. 2021. J Leukoc Biol. 110:1171. PubMed
  17. Xiao C, et al. 2022. iScience. 25:103934. PubMed
  18. Vidard L, et al. 2023. Immun Inflamm Dis. 11:e749. PubMed
  19. Sun Y, et al. 2023. Sci Adv. 9:eade7151. PubMed
  20. Jenkins E, et al. 2023. Nat Commun. 14:1611. PubMed
  21. Xiao C, et al. 2023. Nat Aging. 3:418. PubMed
RRID
AB_1877228 (BioLegend Cat. No. 343305)
AB_1877227 (BioLegend Cat. No. 343306)

Antigen Details

Structure
Ig superfamily
Distribution

A*02 individuals leukocytes

Function
Antigen presentation
Ligand/Receptor
CD8, CD3/TCR
Cell Type
Leukocytes
Biology Area
Immunology
Molecular Family
MHC Antigens
Gene ID
3105 View all products for this Gene ID
UniProt
View information about HLA-A2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 02/15/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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