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PE anti-mouse CD4 Antibody

Pricing & Availability
Clone
GK1.5 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2b, κ
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Product Citations
publications
1_GK1dot5_PE_021706
C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) PE (filled histogram) or rat IgG2b, κ PE isotype control (open histogram).
  • 1_GK1dot5_PE_021706
    C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) PE (filled histogram) or rat IgG2b, κ PE isotype control (open histogram).
  • 2_GK15_PE_CD4_Antibody_2_040122
    C57BL/6 mouse splenocytes were stained with CD4 (clone GK1.5) PE (solid line) or rat IgG2b, κ PE isotype control (dashed line).
See PE spectral data
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100407 50 µg 20€
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100408 200 µg 58€
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Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosin kinase, lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CTL clone V4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD4 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of CD4+ T cell activation1,4,11, thymocyte costimulation3, in vitro and in vivo depletion2,5-8, blocking of egg-sperm cell adhesion1,4, immunohistochemical staining of acetone-fixed frozen sections9,10, immunoprecipitation1,2, and spatial biology (IBEX)12,13. The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. Binding of GK1.5 antibody to CD4 T cells can be blocked by RM4-5 antibody, but not RM4-4 antibody. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100442) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Dialynas DP, et al. 1983. J. Immunol. 131:2445. (Block, IP)
  2. Dialynas DP, et al. 1983. Immunol. Rev. 74:29. (IP, Deplete)
  3. Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim)
  4. Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block)
  5. Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete)
  6. Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete)
  7. Ghobrial RR, et al. 1989. Clin. Immunol. Immunopathol. 52:486. (Deplete)
  8. Israelski DM, et al. 1989. J. Immunol. 142:954. (Deplete)
  9. Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC)
  10. Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC)
  11. Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block)
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Palacios-Arreola M, et al. 2017. Sci Rep. 10.1038/s41598-017-10135-1. PubMed
  2. Yeon S, et al. 2017. Sci Rep. 10.1038/s41598-017-11492-7. PubMed
  3. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  4. Hönes J, et al. 2017. Sci Rep. . 10.1038/s41598-017-15866-9. PubMed
  5. Fatkhullina AR et al. 2018. Immunity. 49(5):943-957 . PubMed
  6. Lee JS et al. 2018. Cell. 174(6):1559-1570 . PubMed
  7. Gaya M et al. 2018. Cell. 172(3):517-533 . PubMed
  8. Chen S et al. 2018. Cell reports. 25(7):1729-1740 . PubMed
  9. Xia Y, et al. 2018. Cell. 175:1059. PubMed
  10. Ren W, et al. 2018. Mucosal Immunol. 12:531. PubMed
  11. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  12. Cai Y, et al. 2019. Cell Rep. 27:3034. PubMed
  13. Wang J, et al. 2019. Aging (Albany NY). 11:3463. PubMed
  14. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  15. Tomay F, et al. 2019. J Transl Med. 17:237. PubMed
  16. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  17. Niemann J, et al. 2019. Nat Commun. 10:3236. PubMed
  18. Du Q, et al. 2019. Front Microbiol. 10:2050. PubMed
  19. Li W, et al. 2019. Nat Commun. 10:3349. PubMed
  20. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  21. Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
  22. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
  23. Isoda T et al. 2017. Cell. 171(1):103-119 . PubMed
  24. Pérez‐Mazliah D et al. 2017. EBioMedicine. 24:216-230 . PubMed
  25. Man SM et al. 2016. Cell. 167(2):382-396 . PubMed
  26. Matsuo K, et al. 2018. J Immunol. 200:800. PubMed
  27. Yuan X, et al. 2019. JCI Insight. 4:e124317. PubMed
  28. Lercher A, et al. 2019. Immunity. 51:1074. PubMed
  29. Mesin L, et al. 2020. Cell. 180(1):92-106.e11.. PubMed
  30. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  31. Blagih J, et al. 2020. Cell Rep. 30:481. PubMed
  32. Fu S, et al. 2020. Nat Commun. 0.7625. PubMed
  33. Du J, et al. 2018. AMB Express. 8:158. PubMed
  34. Jing Y, et al. 2020. Sci Adv. 6:eaax9455. PubMed
  35. Crowe J, et al. 2020. PLoS Pathog. 16:e1008391. PubMed
  36. Jiang W, et al. 2017. Sci Rep. 7:6501. PubMed
  37. Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
  38. Xu X, et al. 2020. Chin Med. 15:33. PubMed
  39. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  40. Bai C, et al. 2020. Mol Ther Oncolytics. 17:9. PubMed
  41. Liu Y, et al. 2019. Sci Rep. 9:18970. PubMed
  42. Ireland L, et al. 2020. Front Immunol. 11:297. PubMed
  43. Ajith A, et al. 2019. FASEB J. 33:5220. PubMed
  44. Pflügler S, et al. 2020. Commun Biol. 3:252. PubMed
  45. Kisielow J, et al. 2019. Nat Immunol. 1.286111111. PubMed
  46. Parhi L, et al. 2020. Nat Commun. 2.721527778. PubMed
  47. Cheng Y, et al. 2020. PLoS Pathog. 16:e1008569. PubMed
  48. McCauley ME, et al. 2020. Nature. 585(7823):96-101. PubMed
  49. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  50. Rudd CE, et al. 2020. Cell Reports. 30(7):2075-2082. PubMed
  51. Leech JM, et al. 2020. Cell Host & Microbe. 26(6):795-809.e5.. PubMed
  52. Sun CC, et al. 2020. Genome Med. 0.553472222. PubMed
  53. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  54. Zhang H, et al. 2009. Invest Ophthalmol Vis Sci. 50:2653. PubMed
  55. Sun J, et al. 2012. Arterioscler Thromb Vasc Biol. 32:15:00. PubMed
  56. Liu J, et al. 2012. PLoS One. 7:e44044. PubMed
  57. Yomogida K, et al. 2013. Biochem Biophys Res Commun. 434:263. PubMed
  58. Mahlios J, Zhuang Y 2011. Mol Immunol. 49:227. PubMed
  59. Yang C, et al. 2013. Proc Natl Acad Sci U S A. 111:109. PubMed
  60. Willinger T, et al. 2014. J Exp Med. 211:685. PubMed
  61. Aebischer D, et al. 2014. PLoS One. 9:99297. PubMed
  62. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  63. Collin R, et al. 2014. J Immunol. 193:3503. PubMed
  64. Nakamura Y, et al. 2015. Infect Immun . 83:671. PubMed
  65. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  66. Hao B, et al. 2015. J Exp Med. 212:809. PubMed
  67. Sanders K, et al. 2015. Cancer Immunol Res. 3: 891-901. PubMed
  68. Sato K, et al. 2015. PLoS One. 10: 0138291. PubMed
  69. Xu Y, et al. 2016. Nat Commun. 7:12073. PubMed
  70. Klose R, et al. 2016. Nat Commun. 7:12528. PubMed
  71. Liyanage S, et al. 2016. Exp Eye Res. 151:160-70. PubMed
  72. Han X, et al. 2017. Int J Mol Sci. 10.3390/ijms18050942. PubMed
  73. Zheng DW, et al. 2020. Nat Commun. 3.865972222. PubMed
  74. Kong IY, et al. 2020. Cell Rep. 33:108290. PubMed
  75. Xie Z, et al. 2020. Light Sci Appl. 0.486805556. PubMed
  76. Karki R, et al. 2020. JCI Insight. 5:00. PubMed
  77. Zhang L, et al. 2020. Front Oncol. 1.243055556. PubMed
  78. Bi CS, et al. 2020. Cell Prolif. 53:e12827. PubMed
  79. Haque M, et al. 2021. STAR Protoc. 2:100264. PubMed
  80. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  81. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  82. Han C, et al. 2021. Cell Reports. 34(6):108706. PubMed
  83. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  84. Daneshmandi S, et al. 2021. Cell Reports. 34(10):108831. PubMed
  85. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  86. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  87. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  88. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  89. Qian Y, et al. 2021. Cell Reports. 36(8):109602. PubMed
  90. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  91. Fu Y, et al. 2020. Mol Ther. 1214:28. PubMed
  92. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  93. Fu W, et al. 2020. J Immunol Res. 8820355:2020. PubMed
  94. Gao P, et al. 2021. Nat Commun. 1194:12. PubMed
  95. Wang F, et al. 2021. Neoplasia. 23:281. PubMed
  96. Li X, et al. 2021. Cell Death Dis. 12:314. PubMed
  97. Rodríguez L, et al. 2021. Biomolecules. 11: . PubMed
  98. Zhang Z, et al. 2021. Oncoimmunology. 10:1912472. PubMed
  99. Pereira JL, et al. 2021. Transl Oncol. 14:101125. PubMed
  100. Liu W, et al. 2021. Cell Death Discov. 7:136. PubMed
  101. Wang J, et al. 2021. Am J Cancer Res. 11:2005. PubMed
  102. Ben-Yehuda H, et al. 2021. Mol Neurodegener. 16:39. PubMed
  103. Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
  104. Huang Z, et al. 2021. Nat Commun. 12:145. PubMed
  105. Huang YL, et al. 2021. Commun Biol. 4:229. PubMed
  106. Zhang Q, et al. 2021. Front Cell Dev Biol. 9:655552. PubMed
  107. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  108. Li H, et al. 2021. Nat Commun. 12:2773. PubMed
  109. Chen G, et al. 2021. Sci Adv. 7:eabg5686. PubMed
  110. Xie Z, et al. 2020. Light Sci Appl. 9:161. PubMed
  111. Ma C, et al. 2021. Signal Transduct Target Ther. 6:353. PubMed
  112. Cho K, et al. 2021. iScience. 24:103117. PubMed
  113. Daneshmandi S, et al. 2021. Elife. 10:. PubMed
  114. Tan X, et al. 2021. Front Oncol. 11:768222. PubMed
  115. Zhang J, et al. 2021. MedComm (Beijing). 2:256. PubMed
  116. Endo-Umeda K, et al. 2021. Sci Rep. 11:22595. PubMed
  117. Zhong MC, et al. 2021. J Exp Med. 218:. PubMed
  118. Apte SH, et al. 2020. Clin Transl Immunology. 9:e1209. PubMed
  119. Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed
  120. Jiang W, et al. 2021. Oncol Lett. 22:625. PubMed
  121. Qi J, et al. 2021. Nat Commun. 12:4755. PubMed
  122. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
  123. Hu Y, et al. 2021. J Nanobiotechnology. 19:416. PubMed
  124. Zuo S, et al. 2021. J Immunother Cancer. 9:. PubMed
  125. Li Y, et al. 2022. Clin Transl Immunology. 11:e1362. PubMed
  126. Lv F, et al. 2022. iScience. 25:103639. PubMed
  127. Friess MC, et al. 2022. Cell Rep. 38:110334. PubMed
  128. Zhao X, et al. 2022. Front Mol Biosci. 9:786864. PubMed
  129. Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed
  130. Zuo W, et al. 2022. Cell Death Discov. 8:257. PubMed
  131. Xiang G, et al. 2022. Cell Death Dis. 13:451. PubMed
  132. Antunes KH, et al. 2022. Front Immunol. 13:867022. PubMed
  133. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  134. Zhang Q, et al. 2022. Commun Biol. 5:544. PubMed
  135. Chen YL, et al. 2022. Front Neurosci. 16:876582. PubMed
  136. Li H, et al. 2022. iScience. 25:104481. PubMed
  137. Shen X, et al. 2021. Front Immunol. 12:710750. PubMed
  138. Akazawa S, et al. 2021. Diabetologia. 64:878. PubMed
  139. Kawai H, et al. 2021. Asian Pac J Allergy Immunol. :. PubMed
  140. Lal JC, et al. 2021. Breast Cancer Res. 23:83. PubMed
  141. Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
  142. Li J, et al. 2021. Cell Commun Signal. 19:89. PubMed
  143. Wu H, et al. 2021. Cell Death Discov. 7:225. PubMed
  144. Lee S, et al. 2021. Autophagy. 18:1062. PubMed
  145. Xu Y, et al. 2021. iScience. 24:103445. PubMed
  146. Georgiadou A, et al. 2022. Elife. 11:. PubMed
  147. Okubo A, et al. 2021. Int J Mol Sci. 23:. PubMed
  148. Jie X, et al. 2022. J Immunother Cancer. 10:. PubMed
  149. Wang Y, et al. 2022. Nat Cell Biol. 24:230. PubMed
  150. Feola S, et al. 2022. Front Immunol. 13:826164. PubMed
  151. Xu Z, et al. 2022. Cell Death Dis. 13:502. PubMed
  152. Rive CM, et al. 2022. Mol Ther Methods Clin Dev. 26:4. PubMed
  153. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  154. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  155. Sodji QH, et al. 2022. Cancer Res Commun. 2:725. PubMed
  156. Yang H, et al. 2022. Nutrients. 14:. PubMed
  157. Pantelidou C, et al. 2022. NPJ Breast Cancer. 8:102. PubMed
  158. Harnett MM, et al. 2022. Front Immunol. 13:953053. PubMed
  159. Tsukui H, et al. 2020. BMC Cancer. 20:411. PubMed
  160. Liang Z, et al. 2021. J Cell Physiol. 236:4725. PubMed
  161. Pervaiz N, et al. 2021. Pigment Cell Melanoma Res. 34:918. PubMed
  162. Deliyannis G, et al. 2021. JCI Insight. 6:. PubMed
  163. Petty AJ, et al. 2021. JCI Insight. 6:. PubMed
  164. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  165. Khan IM, et al. 2021. Immunohorizons. 5:703. PubMed
  166. Andrade ACDSP, et al. 2021. J Virol. 95:e0127621. PubMed
  167. Gawish R, et al. 2022. Elife. 11:. PubMed
  168. Le QV, et al. 2022. Bioact Mater. 15:160. PubMed
  169. Cao Y, et al. 2022. Small. 18:e2203466. PubMed
  170. Guo H, et al. 2022. PLoS Pathog. 18:e1010857. PubMed
  171. Colombo M, et al. 2022. iScience. 25:105042. PubMed
  172. Ren Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  173. Tan L, et al. 2022. Aging Dis. 13:1562. PubMed
  174. Bauer KM, et al. 2022. JCI Insight. 7:. PubMed
  175. Chen S, et al. 2022. iScience. 25:105176. PubMed
  176. Greenberg J, et al. 2022. JCI Insight. Online ahead of print. PubMed
  177. Shibata K, et al. 2022. Nat Commun. 13:6948. PubMed
  178. Zveik O, et al. 2022. Glia. 70:1191. PubMed
  179. Yue Y, et al. 2022. Nat Biomed Eng. 6:898. PubMed
  180. Zhou H, et al. 2022. EMBO Rep. 23:e53791. PubMed
  181. Grebinoski S, et al. 2022. Nat Immunol. 23:868. PubMed
  182. Merana GR, et al. 2022. Cell Rep. 39:110891. PubMed
  183. Guo X, et al. 2022. Invest Ophthalmol Vis Sci. 63:12. PubMed
  184. Tessaro FHG, et al. 2022. Cell Rep. 39:110977. PubMed
  185. Shen CY, et al. 2022. Pharmaceutics. 14: . PubMed
  186. Millar FR, et al. 2022. Cell Rep. 41:111596. PubMed
  187. Zhu J, et al. 2022. Nat Commun. 13:7466. PubMed
  188. Shi C, et al. 2023. Nat Nanotechnol. 18:86. PubMed
  189. Yu J, et al. 2022. Nat Commun. 13:7903. PubMed
  190. Zhao X, et al. 2022. STAR Protoc. 3:101859. PubMed
  191. Choi H, et al. 2023. J Nanobiotechnology. 21:5. PubMed
  192. Wang L, et al. 2022. Nutrients. 15: . PubMed
  193. Glaubitz J, et al. 2023. Gut. :. PubMed
  194. Zhao M, et al. 2023. J Nanobiotechnology. 21:50. PubMed
  195. Wang L, et al. 2023. Oxid Med Cell Longev. 2023:6480848. PubMed
  196. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
  197. McCafferty S, et al. 2022. Mol Ther. 30:2968. PubMed
  198. Puth S, et al. 2022. Biomaterials. 286:121542. PubMed
  199. Lin J, et al. 2022. Adv Sci (Weinh). 9:e2202633. PubMed
  200. Zhao Q, et al. 2023. Signal Transduct Target Ther. 8:40. PubMed
  201. Wang F, et al. 2023. Int J Mol Sci. 24:. PubMed
  202. Wang JX, et al. 2023. JCI Insight. 8:. PubMed
  203. Tan X, et al. 2023. Adv Sci (Weinh). 10:e2206768. PubMed
  204. Fu S, et al. 2023. Nat Commun. 14:2248. PubMed
  205. Kaltenbach L, et al. 2023. Nat Immunol. 24:915. PubMed
  206. Wu N, et al. 2023. Front Bioeng Biotechnol. 11:1173381. PubMed
  207. Ji Y, et al. 2023. Nat Cell Biol. 25:726. PubMed
  208. Guo X, et al. 2023. Mol Immunol. 160:32. PubMed
RRID
AB_312692 (BioLegend Cat. No. 100407)
AB_312693 (BioLegend Cat. No. 100408)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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