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PE anti-mouse IgG2a Antibody

Pricing & Availability
Clone
RMG2a-62 (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin G2a
Isotype
Rat IgG
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Product Citations
publications
See PE spectral data
Cat # Size Price Quantity Check Availability Save
407107 25 µg 64€
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407108 100 µg 142€
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Description

The RMG2a-62 monoclonal antibody reacts with immunoglobulin G2a and G2c (IgG2a, IgG2c) and in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG2a-62 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Gavin AL, et al.2006. Science 314:1936.
  2. Podolin PL, et al. 2008. J. Immunol. 180:7989. (IP) PubMed
Product Citations
  1. Lau KX, et al. 2020. Nat Commun. 11:2420. PubMed
  2. Eder JM, et al. 2020. Sci Rep. 10:4045. PubMed
  3. An B, et al. 2020. Front Cell Dev Biol. 8:262. PubMed
  4. Aguilar–Briseñ o JA, et al. 2020. Nat Commun. 2.664583333. PubMed
  5. Habets T, et al. 2016. PLoS One. 11: 0159515. PubMed
  6. Rodriguez AB, et al. 2021. Cell Reports. 36(3):109422. PubMed
  7. Kim YJ, et al. 2021. Int J Mol Sci. :22. PubMed
  8. Zirngibl F, et al. 2021. J Immunother Cancer. 9:. PubMed
  9. Song S, et al. 2021. Front Immunol. 12:705140. PubMed
  10. Stabel JR, et al. 2022. PLoS One. 17:e0278313. PubMed
  11. Tan TCJ, et al. 2022. Nat Commun. 13:7796. PubMed
  12. Ivasko SM, et al. 2023. Front Immunol. 13:1023206. PubMed
RRID
AB_10549974 (BioLegend Cat. No. 407107)
AB_10549456 (BioLegend Cat. No. 407108)

Related FAQs

IgG2a gene is deleted in some mouse strains, which ones are they?

IgG2a gene is deleted in C57Bl/6, C57Bl/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 01/29/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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