PE/Dazzle™ 594 anti-mouse CD19 Antibody

Product Details

Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse CD19-expressing K562 human erythroleukemia cells

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence⁷.

Application References

(PubMed link indicates BioLegend citation)

1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
12. Olive V, et al. 2013. Elife. 2:822. PubMed
13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed

Product Citations

1. Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
2. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
3. Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
4. Yeung F, et al. 2020. Cell Host & Microbe. 27(5):809-822. PubMed
5. Lee H, et al. 2020. Cell Metabolism. 31(4):822-836. PubMed
6. Lees JG, et al. 2020. PLoS One. 15:e0238164. PubMed
7. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
8. Baier FA, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:745. PubMed
9. França TT, et al. 2021. JCI Insight. 6:. PubMed
10. Kuhn JA, et al. 2021. Elife. 10:. PubMed
11. Lingegowda H, et al. 2022. Front Reprod Health. 3:726936. PubMed
12. Geng T, et al. 2022. Methods Mol Biol. 2585:71. PubMed
13. Jebbawi F, et al. 2021. Parasite Immunol. 43:e12834. PubMed
14. van Dierendonck XAMH, et al. 2022. Proc Natl Acad Sci U S A. 119:e2114739119. PubMed
15. Zhao K, et al. 2023. Front Immunol. 14:1101769. PubMed
16. Rocca Y, et al. 2023. Methods Mol Biol. 2618:187. PubMed
17. Mabire M, et al. 2023. Nat Commun. 14:1830. PubMed
18. Wang Z, et al. 2023. J Cancer. 14:1049. PubMed

RRID

AB_2564000 (BioLegend Cat. No. 115553)
AB_2564001 (BioLegend Cat. No. 115554)

Antigen Details

Structure

Ig superfamily, associates with CD21 and CD81, 95 kD

Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function

Modulates B cell activation and differentiation

Ligand/Receptor

CD21, CD81, Leu-13

Cell Type

B cells, Dendritic cells

Biology Area

Costimulatory Molecules, Immunology

Molecular Family

CD Molecules

Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID

12478 View all products for this Gene ID

UniProt

View information about CD19 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Pages & Pathways

Related FAQs

Other Formats

Concentration & Expiration Lookup

Certificate of Analysis