Loading...

Purified anti-GFP Epitope Tag Antibody (Previously Covance catalog# MMS-118P)

Pricing & Availability
Clone
B34 (See other available formats)
Regulatory Status
RUO
Other Names
Green Fluorescent Protein
Previously
Covance Catalog# MMS-118P
Isotype
Mouse IgG1
Ave. Rating
Submit a Review
Product Citations
publications
B34_Purified_GFP_Antibody_WB_032715
Western Blot showing specificity of Green Fluorescent Protein (GFP) (clone B34) 0.1 µg/ml . Lane 1: Molecular weight marker. Lane 2: 0.1 µg GFP. Lane 3: 0.05 µg GFP. Lane 4: BSA neg control.
  • B34_Purified_GFP_Antibody_WB_032715
    Western Blot showing specificity of Green Fluorescent Protein (GFP) (clone B34) 0.1 µg/ml . Lane 1: Molecular weight marker. Lane 2: 0.1 µg GFP. Lane 3: 0.05 µg GFP. Lane 4: BSA neg control.
Cat # Size Price Quantity Check Availability Save
902605 25 µL 72€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
902601 200 µL 285€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
902602 500 µL 445€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Green Fluorescent Protein (GFP) is a single chain 238 amino acid protein derived from the jellyfish Aequoria victoria. GFP emits a bright green fluorescence when illuminated with UV or blue light and is a useful tool for cellular and developmental biologists. Unlike other bioluminescent molecules, GFP does not require additional proteins, substrates or co-factors for its activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Aequorea victoria,Epitope tag
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Antibody B34, generated against purified wild type GFP, offers an alternitive for detecting GFP and GFP variants including enhanced/mutant GFP.
Formulation
Phosphate-buffered solution + 0.03% Thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB, Purification
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting.

The optimal working dilution should be determined for each specific assay condition.
• WB: 1:5,000*
 

Application Notes

This antibody is effective in immunoblotting (WB) of GFP labeled proteins.

*Predicted MW = 27 kD

Application References

(PubMed link indicates BioLegend citation)
  1. Pecot MY, Malhotra V. 2004. Cell. 116:99.
  2. Kessels MM, et al. 2001. J Cell Biol. 153:351.
  3. Gerdes HH, Kaether C. 1996. FEBS Lett. 389:44.
  4. Davis I, et al. 1995. Dev Biol. 170:726.
  5. Chalfie M. 1995. Photochem Photobio. 62:651.
  6. Stearns T. 1995. Curr Biol. 5:262.
  7. Chalfie M, et al. 1994. Science. 263:802.
  8. Lavy M, et al. 2007. Curr. Biol. 17:947. (WB)
  9. Hobl B, et al. 2013. Protein Expr. Purif. 10:32. (WB)
  10. Zhang Y, et al. 2007. J. Biol. Chem. 282:13022. (WB) PubMed
  11. Asante D, et al. 2013. J. Cell. Sci. 126:5189. (WB) PubMed
  12. Oh Y, et al. 2013. Mol Biol Cell. 24:1305. (WB) PubMed
  13. Wloka C, et al. 2013. J. Cell. Biol. 200:271. (WB)
  14. Redfern RE, et al. 2010. Protein Sci. 19:1948. (WB) PubMed
Product Citations
  1. Shearer RF, et al. 2018. Mol Biol Cell. 29:1542. PubMed
  2. Kennedy SP, et al. 2019. Breast Cancer Res. 21:43. PubMed
  3. Borgermann N et al. 2019. The EMBO journal. 38(8) pii: e101496. PubMed
  4. Shen K, et al. 2019. Mol Cell. 74:729. PubMed
  5. Sakamaki JI, et al. 2017. Mol Cell. 66:517. PubMed
  6. Robles‐Valero J et al. 2017. Cancer cell. 32(5):608-623 . PubMed
  7. Stehle D, et al. 2018. Sci Rep. 8:12434. PubMed
  8. Bauer CK, et al. 2019. Am J Hum Genet. 104:1139. PubMed
  9. Bhatter N, et al. 2019. FEBS J. 286:4693. PubMed
  10. Bhatter N, et al. 2020. Wellcome Open Res. 3:102. PubMed
  11. Costé de Bagneaux P, et al. 2020. PLoS Genet. 16:e1008625. PubMed
  12. Liu Y, et al. 2020. Nat Commun. 11:589. PubMed
  13. McCaughey J, et al. 2019. J Cell Biol. 218:929. PubMed
  14. Redfern R, et al. 2010. Protein Sci. 19:1948-1956. PubMed
  15. Oh Y, et al. 2013. Mol Biol Cell. 24:1305-1320. PubMed
  16. Asante D, et al. 2013. J Cell Sci. 126:5189-5197. PubMed
  17. Zhang Y, Barbieri Q 2007. J Biol Chem. 282:13022-13032. PubMed
  18. Zhang X, et al. 2016. Nat Commun. 7:12109. PubMed
  19. Croucher D, et al. 2016. Sci Signal. 9: ra69. PubMed
  20. Pogorelko G, et al. 2016. PLoS One. 11: 0159757. PubMed
  21. Kravchick D, et al. 2016. EMBO J. 35: 1923 - 1934. PubMed
  22. Eshleman N, et al. 2016. RNA. 22: 1200 - 1214. PubMed
  23. Wang Y, et al. 2016. Sci Rep. 6:37213. PubMed
  24. Manjunath LE, et al. 2020. J Biol Chem. 295:17009. PubMed
  25. Dhingani N, et al. 2020. Sci Rep. 10:18648. PubMed
  26. Herger A, et al. 2020. PLoS Genet. 16:e1008847. PubMed
  27. den Brave F, et al. 2020. Cell Rep. 31:107680. PubMed
  28. Arthur AL, et al. 2021. Elife. 10: . PubMed
  29. Manjunath LE, et al. 2020. J Biol Chem. 295:17009. PubMed
  30. Sánchez-Martín J, et al. 2021. Nat Plants. 7:327. PubMed
  31. Garnelo Gómez B, et al. 2021. J Cell Sci. 134:. PubMed
  32. Basic M, et al. 2021. J Biol Chem. 297:101263. PubMed
  33. Sánchez-Solana B, et al. 2021. Mol Cancer. 20:141. PubMed
  34. Schmitz F, et al. 2021. Sci Rep. 11:19232. PubMed
  35. Ulamec SM, et al. 2022. Nat Commun. 13:4986. PubMed
  36. Chattopadhyay S, et al. 2022. Nat Commun. 13:7184. PubMed
  37. von Elsner L, et al. 2022. Brain. 145:1551. PubMed
  38. Shak C, et al. 2023. J Cell Sci. 133: . PubMed
  39. Grochowska KM, et al. 2023. EMBO J. 42:e112453. PubMed
RRID
AB_2734671 (BioLegend Cat. No. 902605)
AB_2565021 (BioLegend Cat. No. 902601)
AB_2565022 (BioLegend Cat. No. 902602)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about GFP on UniProt.org
Go To Top Version: 7    Revision Date: 09/20/2023

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account