- Clone
- 4B4-1 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VI C-7
- Other Names
- 4-1BB, ILA, CD137, TNFRSF9
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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PHA-stimulated (3 days) human peripheral blood mononuclear cells stained with purified 4B4-1, followed by biotinylated anti-mouse IgG and Sav-PE
Cat # | Size | Price | Quantity Check Availability | Save | ||
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309802 | 100 µg | 105€ |
CD137 is a 39 kD transmembrane protein also known as 4-1BB. It is expressed on activated T cells. CD137 is a type I membrane protein and a member of the tumor necrosis factor receptor superfamily. CD137 appears to be important for T cell proliferation and survival, and induces monocyte activation through its interaction with 4-1BB ligand.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Ectodomain of recombinant human 4-1BB fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
IP, ELISA - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications (for the relevant formats) include: immunoprecipitation1,4, inhibition of cytokine production2,3, and ELISA. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 309804) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by Streptavidin-PE (Cat. No. 405204)).
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Application References
(PubMed link indicates BioLegend citation) -
- Garni-Wagner B, et al. 1996. Cell. Immunol. 169:91. (IP)
- Salih HR, et al. 2000. J. Immunol. 165:2903. (FA)
- Kienzle G, et al. 2000. Int. Immunol. 12:73. (FA)
- Langstein J, et al. 1998. J. Immunol. 160:2488. (IP)
- Product Citations
- RRID
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AB_314781 (BioLegend Cat. No. 309802)
Antigen Details
- Structure
- TNFR superfamily, type I transmembrane protein, 30 kD
- Distribution
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Activated T cells
- Function
- T cell costimulation
- Ligand/Receptor
- 4-1BB ligand
- Cell Type
- T cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
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1. Gruss H, et al. 1995. Blood 85:3378.
2. Sica G, et al. 2000. Adv. Exp. Med. Biol. 465:355.
3. Alderson M, et al. 1994. Eur. J. Immunol. 24:2219.
4. Schwarz H, et al. 1996. Blood 87:2839. - Gene ID
- 3604 View all products for this Gene ID
- UniProt
- View information about CD137 on UniProt.org
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