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Purified anti-human CD163 Antibody

Pricing & Availability
Clone
GHI/61 (See other available formats)
Regulatory Status
RUO
Workshop
VI M38
Other Names
GHI/61, M130, RM3/1, p155, Hemoglobin/Haptoglobin Complex Receptor, macrophage-associated antigen, ED2(rat)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
GHIslash61_Pure_071108.jpg
Human peripheral blood monocytes stained with purified GHI/61, followed by anti-mouse IgG FITC
  • GHIslash61_Pure_071108.jpg
    Human peripheral blood monocytes stained with purified GHI/61, followed by anti-mouse IgG FITC
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333602 100 µg 95€
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Description

CD163 is a member of the group B scavenger receptor cysteine-rich superfamily, also known as GHI/61, M130, RM3/1, p155, hemoglobin-haptoglobin complex receptor, or macrophage-associated antigen. It is a 134 kD (non-reduced)/155 kD (reduced) glycoprotein primarily expressed on macrophages, Kupffer cells, monocytes, a subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells. CD163 binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages. Membrane CD163 can be cleaved by metalloproteinases (MMP), resulting in a soluble form. Elevated serum level of sCD163 has been implicated in many kinds of inflammatory diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone GHI/61 binds to domain 7 of CD163. Additional reported applications (for the relevant formats) include: immunocytochemical staining, immunoprecipitation, western blot1, and spatial biology (IBEX)6,7.

Application References

(PubMed link indicates BioLegend citation)
  1. Pulford K, et al. 1992. Immunology 75:588. (ICC, IP, WB)
  2. Law SK, et al. 1993. Eur. J. Immunol. 23:2320.
  3. Madsen M, et al. 2004. J. Biol. Chem. 279:51561.
  4. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
  5. Buttari B, et al. 2011. Atherosclerosis. 215:316. PubMed
  6. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  7. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
  2. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  3. Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed
  4. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  5. Keskin DB, et al. 2019. Nature. 565:234. PubMed
  6. Correia CR, et al. 2017. Biomater Sci. 5:551. PubMed
  7. Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed
  8. Stras SF, et al. 2020. Developmental Cell. 51(3):357-373.e5.. PubMed
  9. Buttari B, et al. 2011. Atherosclerosis. 215:316. PubMed
  10. Greven D, et al. 2015. Ann Rheum Dis. 74: 1924 - 1930. PubMed
  11. Friebel E, et al. 2020. Cell. 181(7):1626-1642.e20. PubMed
  12. Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
  13. Camiolo MJ, et al. 2021. Cell Reports. 35(2):108974. PubMed
  14. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  15. Kim J, et al. 2022. Sci Rep. 12:7753. PubMed
  16. Hu G, et al. 2021. Nat Commun. 12:773. PubMed
  17. Syrimi E, et al. 2021. iScience. 24:103215. PubMed
  18. Farhadian SF, et al. 2022. JCI Insight. 7:. PubMed
  19. Gadalla R, et al. 2022. STAR Protoc. 3:101643. PubMed
  20. Warren CJ, et al. 2022. Cell. 185:3980. PubMed
RRID
AB_1088991 (BioLegend Cat. No. 333602)

Antigen Details

Structure
134 kD (non-reduced)/155 kD (reduced) glycoprotein, scavenger receptor superfamily
Distribution

Monocytes, macrophages, Kuffer cells, subset of dendritic cells, subset of hematopoietic stem/progenitor cells

Function
Clearance of haptoglobin-hemoglobin complex, regulation of cytokine production by macrophages
Ligand/Receptor
Haptoglobin-hemoglobin complex, TWEAK
Cell Type
Dendritic cells, Hematopoietic stem and progenitors, Macrophages, Monocytes
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Roth J, et al. 1994 Transolantation. 57:127
2. Van den Heuvel MM, et al.1999 J. Leukoc. Biol. 66:858
3. Sulahian TH, et al. 2000 Cytokines 12:1312
4. Fabriek BO, et al. 2007 J. Neuroimmunol. 187:179

Gene ID
9332 View all products for this Gene ID
UniProt
View information about CD163 on UniProt.org
Go To Top Version: 4    Revision Date: 08/25/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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