- Clone
- Bu32 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- V CD21.4, VI CD21.5
- Other Names
- Complement C3d receptor (C3dR), complement receptor 2 (CR2), Epstein-Barr virus receptor
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1_Bu32_Pure_070212 1_Bu32_Pure_070212](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/Bu32_Pure_070212.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Human peripheral blood lymphocytes were stained with purified CD21 (clone Bu32) (filled histogram) or mouse IgG1, κ isotype control (dashed histogram), followed by anti-mouse IgG FITC. -
IHC staining of purified anti-human CD21 antibody (clone Bu32) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using 0.1 M Tris-buffered saline with Tween 20 (Cat. No. 925501), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG (yellow) (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 20x objective. Scale bar: 100 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
354902 | 100 µg | 90€ |
CD21 is a 145 kD transmembrane protein also known as complement C3d receptor (C3dR), complement receptor 2 (CR2), and Epstein-Barr virus receptor. CD21 is expressed on B cells, follicular dendritic cells, subsets of normal thymocytes and T cells, and some epithelial cells. CD21 is the receptor used by Epstein-Barr virus to infect B cells and is also the complement receptor for C3d. CD21 has also been shown to interact with a number of proteins, including CD23, CD19, annexin VI, CD81, iC3b, complement receptor 1 (CR1, CD35), and interferon-alpha 1 (IFN-α1).
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-P - Verified
IHC-F - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4.
-
Application References
(PubMed link indicates BioLegend citation) -
- Björck P, et al. 1993. Eur. J. Immunol. 23:1771.
- Frémeaux-Bacchi V, et al. 1996. Eur. J. Immunol. 26:1497.
- Ling NR, et al. 1995. Clin. Exp. Immunol. 101:369.
- Wang, C, et al. 2011. BMC Immunol. 12:53. (IHC)
- Product Citations
- RRID
-
AB_11219188 (BioLegend Cat. No. 354902)
Antigen Details
- Structure
- Transmembrane protein, multiple SUSHI domains, approximate molecular weight 145 kD
- Distribution
-
Expressed on B cells, follicular dendritic cells, subsets of normal thymocytes and T cells, some epithelial cells
- Function
- Epstein-Barr virus receptor on B cells used for infection
- Interaction
- CD23, CD19, complement component 3, annexin VI, CD81, iC3b, complement receptor 1, interferon alpha 1
- Ligand/Receptor
- C3d, EBV
- Cell Type
- B cells, Dendritic cells, Epithelial cells, T cells, Thymocytes
- Biology Area
- Cell Biology, Complement, Costimulatory Molecules, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Kishimoto T, Eds. 1997. Leukocyte Typing VI. Garland Publishing Inc.
2. Moore MD, et al. 1987. Proc. Natl. Acad. Sci. USA 84:9194.
3. Szakonyi G, et al. 2001. Science 292:1725.
4. Weis JJ, et al. 1984. Proc. Natl. Acad. Sci. USA 81:881. - Gene ID
- 1380 View all products for this Gene ID
- UniProt
- View information about CD21 on UniProt.org
Customers Also Purchased
![](https://d1spbj2x7qk4bg.cloudfront.net/Files//Images/media_assets/products/product_images/hi9a.jpg)
![](https://d1spbj2x7qk4bg.cloudfront.net/Files//Images/media_assets/products/product_images/L243_Purified_HLA-DR_Antibody_012519_updated.png)
![](https://d1spbj2x7qk4bg.cloudfront.net/Files//Images/media_assets/products/product_images/MHM-88_Pure_020708.jpg)
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Follow Us