- Clone
- 67A4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- E-Cadherin, cadherin-1, and UVO
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![67A4_Purified_CD324_Antibody_1_121817 67A4_Purified_CD324_Antibody_1_121817](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/67A4_Purified_072307.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human colon carcinoma cell line (HT29) stained with purified CD324 (clone 67A4) (filled histogram), or purified mouse IgG1, κ (open histogram) followed by anti-mouse IgG FITC. -
BT474 breast cancer cells were stained with anti-human CD324 (clone 67A4) using 1:100 dilution, followed by DyLight™ 649 anti-mouse Ig secondary antibody (red) plus DAPI staining for nuclei (blue). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, blocked with 10% serum, and incubated O/N at 4°C. Images were taken under 20x (filter set: EX 647/10x, Dichroic 665LP, EM 700/70x) at exposure 4s. Data provided by John Nolan and Er Liu, La Jolla Bioengineering Institute. -
Total cell lysate (15 µg) from HeLa (negative control, lane 1), MCF-7 (lane2) and NIH3T3 (lane3) were resolved by electrophoresis (4-20% Tris-Glycine), transferred to nitrocellulose, and probed with 1:500 (1 µg/mL), 1:2000 (0.25 µg/mL) and 1:5000 (0.1 µg/mL) diluted purified anti-human CD324 (E-Cadherin) Antibody (clone 67A4). Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP (Cat. No. 405306) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin Antibody (Cat No. 643807) was used as a loading controls (lower). Lane M is the MW ladder.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
324101 | 25 µg | 81€ | ||||
324102 | 100 µg | 165€ |
The 67A4 antibody recognizes human CD324 also known as E-cadherin, cadherin-1, and UVO. CD324, a member of the cadherin superfamily, is a calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of 4 extracellular cadherin repeats and a highly conserved cytoplasmic tail region with a predicted molecular weight of approximately 100 kD. CD324 is widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas, as well as erythroid cells. CD324 functions as a cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. In bacterial pathogenesis, the ectodomain of CD324 mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization. CD324 binds to the αEβ7 integrin to mediate cell adhesion and also interacts with a number of intracellular proteins including including erbin, ezrin, caspase-3, caspase 8, β-catenin, presenilin 1, casein kinase II , as well as other extracellular proteins including the EGF receptor. CD324 is phosphorylated on multiple residues (S857, S866, S870, S872), and can be proteolytically cleaved at reside D769 by caspase-3. The 67A4 antibody has been shown to be useful for flow cytometry.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- T-47D cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
ICC, WB - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml (dilution 1:5000 - 1:500). It is recommended that the reagent be titrated for optimal performance for each application.
It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines. -
Application References
(PubMed link indicates BioLegend citation) -
- Armeanu S, et al. 1995. J. Cell Biol. 131:243.
- Bühring HJ, et al. 1996. Leukemia 10:106.
- Yauch RL, et al. 2005. Clin. Cancer Res. 11:8686. (WB)
- Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
- Ardehali R, et al. 2013. PNAS. 110:3405. PubMed
- Rasanen K, et al. 2013. Mol Cell Protecomics. 12:3778. PubMed
- Chaudhury A, et al. 2014. Nucleic Acids Res. 42:86. PubMed
- Milne P, et al. 2015. Blood. 125:470. PubMed
- Product Citations
- RRID
-
AB_756063 (BioLegend Cat. No. 324101)
AB_756064 (BioLegend Cat. No. 324102)
Antigen Details
- Structure
- Member of the cadherin superfamily. Calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of 4 extracellular cadherin repeats and a highly conserved cytoplasmic tail region. Predicted molecular weight approximately 100 kD.
- Distribution
-
Widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas, as well as erythroid cells
- Function
- Cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. The ectodomain of CD324 mediates bacterial adhesion to mammalian cell, while the cytoplasmic domain is required for internalization.
- Interaction
- Interacts with a variety of proteins including erbin, ezrin, caspase-3, caspase 8, EGF receptor, β-catenin, presenilin 1, casein kinase II, and others
- Ligand/Receptor
- αEβ7 integrin
- Modification
- Phosphorylated on multiple residues (S857, S866, S870, S872), proteolytically cleaved at reside D769 by caspase-3.
- Cell Type
- Embryonic Stem Cells, Epithelial cells, Erythrocytes
- Biology Area
- Cell Biology, Immunology, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Overduin M, et al. 1995. Science 267:386.
2. Boggon TJ, et al. 2002. Science 296:1303.
3. Berx G, et al. 1995. EMBO J. 14:6107.
4. Perl AK, et al. 1998. Nature 392:190. - Gene ID
- 999 View all products for this Gene ID
- UniProt
- View information about CD324 on UniProt.org
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Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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