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Purified anti-human CD8a Antibody

Pricing & Availability
Clone
HIT8a (See other available formats)
Regulatory Status
RUO
Workshop
V CD08.10
Other Names
T8, Leu2
Isotype
Mouse IgG1, κ
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Product Citations
publications
HIT8a_Purified_060807
Human peripheral blood lymphocytes stained with purified HIT8a, followed by anti - mouse IgGs FITC
  • HIT8a_Purified_060807
    Human peripheral blood lymphocytes stained with purified HIT8a, followed by anti - mouse IgGs FITC
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300901 25 µg 20€
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300902 100 µg 44€
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Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone HIT8a recognizes the alpha chain of CD85. It does not block the binding of RPA-T8 antibody to CD8a.

Additional reported applications of this antibody (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5,6. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Awasthi, S., et al. 2011. J. Virol 85:10472. PubMed
  5. Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (IHC, epitope)
  6. Suzuki F, et al. 2012. Arthritis Res. Ther. 14:R48. (IHC)
Product Citations
  1. Agrawal N, et al. 2018. Front Immunol. 2.053472222. PubMed
  2. Li Y, et al. 2019. Front Immunol. 0.460416667. PubMed
  3. Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed
  4. Freund L, et al. 2020. J Invest Dermatol. 1455:140. PubMed
  5. Chiou SH, et al. 2021. Immunity. 54:586. PubMed
  6. Song S, et al. 2021. Commun Biol. 4:1338. PubMed
  7. Ibidapo-Obe O, et al. 2020. Cell Mol Gastroenterol Hepatol. 9:661. PubMed
  8. Gao H, et al. 2021. J Virol. :. PubMed
  9. Moquin-Beaudry G, et al. 2022. Cell Rep Methods. 2:100153. PubMed
  10. Kondo H, et al. 2022. Front Immunol. 13:836923. PubMed
RRID
AB_314105 (BioLegend Cat. No. 300901)
AB_314106 (BioLegend Cat. No. 300902)

Antigen Details

Structure
Ig superfamily, homodimer or heterodimer with CD8β, 32-34 kD
Distribution

Majority of thymocytes, T cell subset, NK cells

Function
MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor
MHC Class I molecules
Cell Type
Dendritic cells, NK cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
925 View all products for this Gene ID
UniProt
View information about CD8alpha on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 07/13/2015

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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