- Clone
- APA5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PDGF receptor-α, PDGFR-α
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_APA5_PE_112409 A_APA5_PE_112409](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A_APA5_PE_112409.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Mouse fibroblast cell line NIH/3T3 stained with APA5 PE -
C57BL/6 splenocytes stained with APA5 PE
Cat # | Size | Price | Quantity Check Availability | Save | ||
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135901 | 25 µg | 44€ | ||||
135902 | 100 µg | 86€ |
Platelet-derived growth factor receptor-α (PDGFR-α), CD140a, is one of two receptors for platelet-derived growth factors (PDGFs) and binds to all isoforms of PDGFs: PDGF-AA, PDGF-AB, and PDGF-BB. PDGFRa is a receptor tyrosine kinase that forms homodimers or heterodimers on the surface upon ligand binding and phosphorylates substrates. PDGFRs consist of either homodimers of α/α, β/β, or heterodimers of α/β. PDGF receptors, α and β, are single glycoproteins with intracellular tyrosine kinase domain. Their ligand, PDGF, is a mitogen for connective tissue and glial cells. CD140a is expressed on embryonic tissues and mesenchymal-derived cells of adult mice. PDGF plays a role in wound healing and acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes, and neutrophils.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse PDGFR-α-hIgG1 recombinant fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
WB, IHC-P, IHC-F - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported (for relevant formats) applications include: Western Blot, blocking function2, and immunohistochemical staining of paraffin and frozen sections. The LEAF™ purified antibody is recommended for functional assays.
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Application References
(PubMed link indicates BioLegend citation) -
- Takakura N, et al. 1996. J. Invest. Dermatol. 107:770.
- Liao C, et al. 2010. J. Clin. Invest. 120:242. (Block)
- Chen H, et al. 2015. ASN Neuro 8:7. PubMed.
- Miyawaki T, et al. 2004. J Neurosci. 24(37): 8124-34. (IHC-F)
- Product Citations
- RRID
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AB_1953327 (BioLegend Cat. No. 135901)
AB_1953328 (BioLegend Cat. No. 135902)
Antigen Details
- Structure
- Alpha chain of the platelet-derived growth factor receptor, a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface after ligand binding.
- Distribution
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Expressed on embryonic tissues and mesenchymal-derived cells of adult mouse.
- Function
- Play a role in wound healing and act as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils.
- Ligand/Receptor
- PDGFs
- Cell Type
- Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
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1. Mukouyama YS, et al. 2006. Proc Natl Acad Sci USA. 103(5):1551
2. Miyawaki T, et al. 2004. J Neurosci. 24(37):8124
3. Takakura N, et al. 1997. J Histochem Cytochem. 45(6):883 - Gene ID
- 18595 View all products for this Gene ID
- UniProt
- View information about CD140a on UniProt.org
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