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Purified anti-mouse CD152 Antibody

Pricing & Availability
Clone
UC10-4B9 (See other available formats)
Regulatory Status
RUO
Other Names
Cytotoxic T Lymphocyte-Associated Antigen-4 (CTLA-4), Ly-56
Isotype
Armenian Hamster IgG
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Product Citations
publications
UC10-4B9_Purified_CD152_Antibody_052819_updated.png
Con A+IL-2-stimulated C57BL/6 splenocytes (3 days) were stained with CD3 APC and CD152 (clone UC10-4B9) Purified (left) or Purified Armenian Hamster IgG isotype control (right) followed by PE anti-Armenian Hamster IgG.
  • UC10-4B9_Purified_CD152_Antibody_052819_updated.png
    Con A+IL-2-stimulated C57BL/6 splenocytes (3 days) were stained with CD3 APC and CD152 (clone UC10-4B9) Purified (left) or Purified Armenian Hamster IgG isotype control (right) followed by PE anti-Armenian Hamster IgG.
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106302 500 µg 184€
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Description

CD152, also known as CTLA-4 or Ly-56, is a 33 kD member of the immunoglobulin superfamily. It is expressed on activated T and B lymphocytes. CD152 is similar to CD28 in amino acid sequence, structure, and genomic organization and these two receptors share common B7 family counter-receptors (B7-1, B7-2). Whereas CD28 delivers a costimulatory signal in T cell activation, CTLA-4 negatively regulates cell-mediated immune responses. CD152 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse CTLA-4-mouse IgG2a fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, ELISA, Block - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The UC10-4B9 antibody can enhance T cell co-stimulation by blocking CTLA-4 interactions with the B7 co-receptors, favoring CD28 interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro stimulation, in vitro and in vivo blocking1-4 of ligand binding, and as ELISA capture antibody5. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106306) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). The Ultra LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 106327).

Application References

(PubMed link indicates BioLegend citation)
  1. Walunas TL, et al. 1994. Immunity 1:405. (Block, IP)
  2. Cilio CM, et al. 1998. J. Exp. Med. 188:1239. (Block)
  3. Issazadeh S, et al. 1999. J. Immunol. 162:761. (Block)
  4. McCoy K, et al. 1997. J. Exp. Med. 186:183. (Block)
  5. Hsu HC, et al. 2007. J. Immunol. 178:5357. (ELISA Capture)
  6. Sugita S, et al. 2010. Invest. Ophthalmol. Vis. Sci. 51:5783. PubMed
Product Citations
  1. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  2. Wei SC, et al. 2019. Immunity. 50:1084. PubMed
  3. Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed
  4. Goel S, et al. 2017. Nature. 548:471. PubMed
  5. Yang K, et al. 2017. Nature. 548:602. PubMed
  6. Sprouse ML, et al. 2018. JCI Insight. 3:e97322. PubMed
  7. Rosenbaum M, et al. 2019. Nat Commun. 2.05. PubMed
  8. Hu J, et al. 2019. Am J Transl Res. 3.043055556. PubMed
  9. Jiao S, et al. 2020. Cell. 179(5):1177-1190.e13.. PubMed
  10. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  11. Sun Y, et al. 2020. J Immunol. 205:3358. PubMed
  12. Muhammad F, et al. 2020. J Autoimmun. 111:102441. PubMed
  13. Sato Y, et al. 2021. BMC Cancer. 21:1222. PubMed
  14. Sudan R, et al. 2022. Front Immunol. 13:830961. PubMed
  15. Liu CJ, et al. 2020. Neuro Oncol. 22:1276. PubMed
  16. Moore AR, et al. 2022. Cell Rep. 41:111651. PubMed
  17. Hezaveh K, et al. 2022. Immunity. 55:324. PubMed
RRID
AB_313251 (BioLegend Cat. No. 106302)

Antigen Details

Structure
Ig superfamily, 33 kD
Distribution

Activated T cells and B cells

Function
Negative regulator of T cell activation
Ligand/Receptor
CD80 (B7-1), CD86 (B7-2)
Cell Type
B cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Allison JP, et al. 1995. Science 270:932.
3. Waterhouse P, et al. 1995. Science 270:985.
4. Linsley PS, et al. 1991. J. Exp. Med. 174:561.

Gene ID
12477 View all products for this Gene ID
UniProt
View information about CD152 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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