- Clone
- M2-7C10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- MLANA, Melanoma antigen recognized by T-cells 1, antigen LB39-AA, antigen SK29-AA, protein Melan-A
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![M2-7C10_A594_MART-1_Antibody_1_081518 M2-7C10_A594_MART-1_Antibody_1_081518](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/M2-7C10_A594_MART-1_Antibody_1_081518.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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IHC staining of Alexa Fluor® 594 anti-MART-1 antibody (clone M2-7C10) on formalin-fixed paraffin-embedded human melanoma tissue. The tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 10X objective. Scale bar: 100 µm -
IHC staining of Alexa Fluor® 594 anti-MART-1 antibody (clone M2-7C10) on formalin-fixed paraffin-embedded human melanoma tissue. The tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. Nuclei were counterstained with DAPI. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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917905 | 25 µg | 109€ | ||||
917906 | 100 µg | 268€ |
Melan-A, also known as melanoma antigen recognized by T cells 1 (MART-1), antigen LB39-AA, and antigen SK29-AA, is an 18 kD transmembrane protein that is expressed in a large proportion of melanomas and melanoma cell lines.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was generated against a MART-1 fusion protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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IHC-P - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Application Notes
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This antibody is effective in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P).
This antibody recognizes MART-1 (Melan-A) [MART1, MART 1] an 18kD transmembrane protein that is expressed in cells of melanocytic lineage. MART-1 is expressed by a large proportion of melanomas and by melanoma cell lines.
In Immunohistochemistry citrate retrieval has also been verified for use with this clone. -
Application References
(PubMed link indicates BioLegend citation) -
- Shidham VB, et al. 2001. Am. J. Surg. Pathol. 25:1039.
- Fetsch PA, et al. 1999. Cancer Cytopath. 87:37.
- Yu LL, et al. 1999. Cancer. 86:617.
- Beaty MW, et al. 1997. Cancer (Cancer Cytopathology). 81:57.
- Marincola FM, et al. 1996. J. Immunother. Emphasis Tumor Immunol. 19:192.
- Rivoltini L, et al. 1995. J. Immunol. 154:2257.
- Kawakami Y, et al. 1994. J. Exp. Med. 180:347.
- Kawakami Y, et al. 1994. Proc. Natl. Acad, Sci. USA. 91:3515.
- RRID
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AB_2750106 (BioLegend Cat. No. 917905)
AB_2750107 (BioLegend Cat. No. 917906)
Antigen Details
- Biology Area
- Cell Biology, Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Gene ID
- 2315 View all products for this Gene ID
- UniProt
- View information about MART-1 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All MART-1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-MART-1 | M2-7C10 | IHC-P |
Alexa Fluor® 594 anti-MART-1 | M2-7C10 | IHC-P |
Purified anti-MART-1 | M2-7C10 | IHC-P |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-MART-1
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Alexa Fluor® 594 anti-MART-1
IHC staining of Alexa Fluor® 594 anti-MART-1 antibody (clone... IHC staining of Alexa Fluor® 594 anti-MART-1 antibody (clone... -
Purified anti-MART-1
IHC staining of purified anti-MART-1 antibody (clone M2-7C10... IHC staining of purified anti-MART-1 antibody (clone M2-7C10...
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