- Clone
- 16B12 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![16B12_DB-HRP_HA.11_Antibody_WB_042017 16B12_DB-HRP_HA.11_Antibody_WB_042017](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/16B12_DB-HRP_HA11_Antibody_WB_042017.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Total cell lysate from CHO (lane 1) and CHO stably transfected with HA tag fused protein (lane 2) (15 µg/lane) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:10,000 purified anti-HA.11 or 1:10,000 Direct-Blot™ HRP anti-HA.11 Antibody. For the purified anti-HA.11 antibody, proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading controls (lower). Lane M is the MW ladder.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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901520 | 25 µL | 81€ | ||||
901519 | 100 µL | 272€ |
The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106. It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.
Product DetailsProduct Details
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Monoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL.
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
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WB - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:10000. The optimal dilution should be determined by titration for each individual assay of interest.
25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution. - Application Notes
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Additional tested and reported applications of the 16B12 clone for the relevant formats include: western blot (WB), immunocytochemistry (ICC), immunoprecipitation (IP), and flow cytometry (FC).
*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.
This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus. - Application References
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- Kim JY, et al. 2003. J Neurosci. 23:5561. (IP, WB)
- Helliwell SB, et al. 2001. J Cell Biol. 153:649. (WB)
- Bennett BD, et al. 2000. J Biol Chem. 275:37712. (IF, IP, WB)
- Royer Y, et al. 2005. J. Biol. Chem. 29:27251. (FC)
- Smith BA, et al. 2012. Genes Cancer. 3:550. (IHC) PubMed
- Hogarth C, et al. 2015. Biol Reprod. 93:19. PubMed
- Görtz D, et al. 2015. Sci Rep. 5:14685. PubMed
- Wilson C, et al. 2015. PLoS One. 10:0139579. PubMed
- Smith B, et al. 2012. Genes Cancer. 3:550-563. PubMed
- Liu Z, et al. 2016. Nature. 530:98-102. PubMed
- Thoms M, et al. 2016. Nucleic Acids Res. 44:926-39. PubMed
- Kim Y, et al. 2016. Nat Commun. 7:10347. PubMed
- Rodríguez-Escudero M, et al. 2016. PLoS One. 11:0148032. PubMed
- Lehmann W, et al. 2016. Nat Commun. 7:10498. PubMed
- Testoni E, et al. 2016. EMBO Mol Med. 8: 105-16. PubMed
- Padilla S, et al. 2016. Nat Neurosci. 10.1038/nn.4274. PubMed
- Martins J, et al. 2016. J Cell Sci. 129:1271-82. PubMed
- Matak P, et al. 2016. Proc Natl Acad Sci U S A. 113:3428-35. PubMed
- Starokadomskyy P, et al. 2016. Nat Immunol. 17:495-504. PubMed
- Mitxelena J, et al. 2016. Nucleic Acids Res. 44:5557-70. PubMed
- Thongthip S, et al. 2016. Genes Dev. 30:645-59. PubMed
- Aaes T, et al. 2016. Cell Rep. 15:274-87. PubMed
- Hodge C, et al. 2016. J Biol Chem. 291:9396-9410. PubMed
- Alagramam K, et al. 2016. Nat Chem Biol. 12:444-51. PubMed
- Veit G, et al. 2016. PLoS Biol. 14:1002462. PubMed
- Lee B, et al. 2016. Development. 143:1721-31. PubMed
- Douchi D, et al. 2016. Plant Cell. 28:1182-99. PubMed
- Avgousti D, et al. 2016. Nature. 535:173-77. PubMed
- Shin H, et al. 2015. Nature. 534:553-7. PubMed
- Gross G, et al. 2016. Nat Methods. 10.1038/nmeth.3894. PubMed
- Dick M, et al. 2016. Nat Commun. 7:11929. PubMed
- Aldrin-Kirk P, et al. 2016. Neuron. 90:955-68. PubMed
- Fan R, et al. 2016. Nat Med. 22:780-91. PubMed
- Rowald K, et al. 2016. Cell Rep. 15:2679-91. PubMed
- Rampal R, Awasthi A, Ahuja V 2016. Development. 143:2334-43. PubMed
- Faden F, et al. 2016. Nat Commun. 7:12202. PubMed
- Lawson C, et al. 2016. Cancer Res. 76: 3826-37. PubMed
- Szargel R, et al. 2016. Hum Mol Genet. 10.1093/hmg/ddw189.. PubMed
- Damez-Werno D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9623-28. PubMed
- Su P, et al. 2016. J Immunol. 197: 1054-64. PubMed
- Morozumi Y, et al. 2016. J Mol Cell Biol. 8:349-62. PubMed
- Miura Y, et al. 2016. Biochem J. 473:2591-602. PubMed
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- Sanchez M, et al. 2016. J Biol Chem. 291:19760-73. PubMed
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- Barquilla A, et al. 2016. Mol Biol Cell. 27:2757-70. PubMed
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- Gómez-H L, et al. 2016. Nat Commun. 7:13298. PubMed
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- Zhang H, et al. 2016. J Neurosci. 36:11837-50. PubMed
- Zhang M, et al. 2016. Cell Res. 26:1302-19. PubMed
- Baehr C, et al. 2016. J Biol Chem. 291:26875-85. PubMed
- Boehm E, et al. 2016. J Biol Chem. 291:25877-87. PubMed
- Gail Kilroy, et al. 2016. J Biol Chem. 291:27289-97. PubMed
- Hongchen Cai, Aimin Liu 2016. Proc Natl Acad Sci U S A. 113:14751-6. PubMed
- Tam K, et al. 2016. MBio. 7:e02024-16. PubMed
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- Kaufmann T, et al. 2016. J Cell Sci. 129:4607-21. PubMed
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- Matsuo Y, et al. 2016. J Cell Sci. 129:4592-606. PubMed
- Athmer J, et al. 2017. MBio. 10.1128/mBio.02320-16. PubMed
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- K Kataoka, K Mochizuki 2017. J Cell Sci. 130:480-9. PubMed
- Liszczak G, et al. 2017. Proc Natl Acad Sci U S A. 114:681-6. PubMed
- Haggie P, et al. 2017. J Biol Chem. 292:771-85. PubMed
- T Taetzsch, et al. 2017. J Neurosci. 37:70-82. PubMed
- Yagita Y, et al. 2017. J Biol Chem. 292:691-705. PubMed
- Chong Jiang, et al. 2017. J Biol Chem. 292:3137-45. PubMed
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- Oishi A, et al. 2017. Sci Rep. 7:8990. PubMed
- Product Citations
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- RRID
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AB_2749912 (BioLegend Cat. No. 901520)
AB_2686981 (BioLegend Cat. No. 901519)
Antigen Details
- Biology Area
- Cell Biology
- Gene ID
- NA
- Specificity (DOES NOT SHOW ON TDS):
- HA.11
- Specificity Alt (DOES NOT SHOW ON TDS):
- HA.11
- App Abbreviation (DOES NOT SHOW ON TDS):
- WB
- UniProt
- View information about HA.11 on UniProt.org
Related Pages & Pathways
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Other Formats
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![](https://d1spbj2x7qk4bg.cloudfront.net/Files//Images/media_assets/products/product_images/9E10_DirectBlotHRP_cmyc_Antibody_WB_021716.jpg)
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Anti-HA.11 Epitope Tag Affinity Matrix
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Alexa Fluor® 488 anti-HA.11 Epitope Tag
HA tagged CHO cells stained with Alexa Fluor® 488 Labeled An... Transfected RBL1 cells expressing the HA tag on the cell sur... -
Alexa Fluor® 594 anti-HA.11 Epitope Tag
Staining of HA.11 Clone 16B12 Monoclonal Antibody, Alexa Flu... -
Anti-HA.11 Epitope Tag
Immunofluorescence of HA.11 tagged Sbhlp protein. Photo cour... Western blot analysis of cell lysates from CHO and CHO HA st... Immunoprecipitation of HA protein from CHO and CHO-HA stable... Immunofluorescence staining of HeLa cells transfected withou... -
Biotin anti-HA.11 Epitope Tag
Total lysates (15 µg protein) from CHO (lane 1) and CHO HA s... Immunofluorescence of HA-tagged plasmid-transfected A549 cel... -
FITC anti-HA.11 Epitope Tag
Transfected RBL1 cells expressing the HA tag on the cell sur... -
Purified anti-HA.11 Epitope Tag
Western blot of Clone 16B12. Lane 1: Molecular weight marker... Staining of Clone 16B12 on methanol fixed CHO cells transfec... Immunoprecipitation of HA protein from CHO and CHO-HA stable... Transfected RBL1 cells expressing the HA tag on the cell sur... -
Alexa Fluor® 647 anti-HA.11 Epitope Tag
HA tag stably transfected CHO cells were fixed with ice cold... Transfected RBL1 cells expressing the HA tag on the cell sur... -
PE anti-HA.11 Epitope Tag
CHO-K1 cells (open histogram) or HA tag stably transfected c... Transfected RBL1 cells expressing the HA tag on the cell sur... -
Direct-Blot™ HRP anti-HA.11 Epitope Tag
Total cell lysate from CHO (lane 1) and CHO stably transfect... -
Ultra-LEAF™ Purified anti-HA.11 Epitope Tag
Total cell lysate (15 µg protein) from CHO (lane 1) and CHO ... Transfected RBL1 cells expressing the HA tag on the cell sur... -
Brilliant Violet 421™ anti-HA.11 Epitope Tag
CHO cells stably transfected with HA tag were fixed with 4% ... Transfected RBL1 cells expressing the HA tag on the cell sur... -
PE/Dazzle™ 594 anti-HA.11 Epitope Tag
CHO-K1 cells (open histogram) or HA tag stably transfected c... Transfected RBL1 cells expressing the HA tag on the cell sur... -
PE/Cyanine7 anti-HA.11 Epitope Tag
CHO-K1 cells (open histogram) or HA tag stably transfected c... Transfected RBL1 cells expressing the HA tag on the cell sur... -
Pacific Blue™ anti-HA.11 Epitope Tag
CHO-K1 cells (open histogram) or HA tag stably transfected c... Transfected RBL1 cells expressing the HA tag on the cell sur... -
APC anti-HA.11 Epitope Tag
CHO-K1 cells (open histogram) or HA tag stably transfected c... Transfected RBL1 cells expressing the HA tag on the cell sur... -
PerCP/Cyanine5.5 anti-HA.11 Epitope Tag
CHO-K1 cells (open histogram) or HA tag stably transfected c... Transfected RBL1 cells expressing the HA tag on the cell sur... -
TotalSeq™-C1131 anti-HA.11 Epitope Tag
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TotalSeq™-A1131 anti-HA.11 Epitope Tag
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TotalSeq™-B1131 anti-HA.11 Epitope Tag
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