- Clone
- N418 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- αX integrin, integrin αX chain, CR4, p150, ITGAX
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![N418_BV785_061812 N418_BV785_061812](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/N418_BV785_061812.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with I-A/I-E FITC and CD11c (clone N418) Brilliant Violet 785™.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
117335 | 125 µL | 172€ | ||||
117336 | 50 µg | 229€ |
CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse spleen dendritic cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
- Concentration
- µg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.
- Application References
-
- Granucci F, et al. 1997. J. Immunol. 159:1794.
- Stokes RW, et al. 1998. J. Immunol. 160:5514.
- Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
- Ma XT, et al. 2006. Cancer Research 66:1169.
- Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
- Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
- Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
- Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
- You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
- Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
- Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
- Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
- Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
- Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
- Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
- Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
- Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
- Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
- Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
- Gibbins JD, et al. 2014. Blood. 124:2953. PubMed
- White CE, et al. 2015. J Immunol. 194:697. PubMed
- Lu X, et al. 2015. J Immunol. 194:2011. PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
-
- RRID
-
AB_11219204 (BioLegend Cat. No. 117335)
AB_2565268 (BioLegend Cat. No. 117336)
Antigen Details
- Structure
- Integrin α-chain, associates with integrin β2 (CD18), 150 kD
- Distribution
-
Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells
- Function
- Cellular adhesion
- Ligand/Receptor
- iC3b, fibrinogen
- Cell Type
- Dendritic cells, Epithelial cells, NK cells, T cells, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780. - Gene ID
- 16411 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- CD11c
- Specificity Alt (DOES NOT SHOW ON TDS):
- CD11c
- App Abbreviation (DOES NOT SHOW ON TDS):
- FC
- UniProt
- View information about CD11c on UniProt.org
Related FAQs
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-mouse CD11c
C57BL/6 mouse splenocytes stained with N418 APC and PK136 PE -
Biotin anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-... Frozen mouse spleen sections. Endogenous biotin blocking app... -
FITC anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-... C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-... -
PE anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-... C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-... -
Purified anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-... -
Alexa Fluor® 488 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-... -
Alexa Fluor® 647 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with N418 Alexa Fluor® 647 Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen secti... Fixed whole mount mouse epidermis Langerhans cells were stai... Live intravital mouse spleen imaging. PE CD11b (red) (clone ... Confocal image of C57BL/6 mouse spleen sample acquired using... Fixed whole mount mouse spleen was stained with FITC CD172a ... -
PE/Cyanine5 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-... -
PE/Cyanine7 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with APC anti-mouse I-A/I-... -
Brilliant Violet 605™ anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with I-A/I-E FITC and... -
Alexa Fluor® 700 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with N418 Alexa Fluor®... -
Pacific Blue™ anti-mouse CD11c
C57BL/6 mouse splenocytes stained with Pacific Blue™ N... -
APC/Cyanine7 anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with I-A/I-E PE and C... -
PerCP/Cyanine5.5 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with N418 PerCP/Cyanine5.5 -
PerCP anti-mouse CD11c
C57BL/6 mouse splenocytes stained with N418 PerCP and M5/114... -
Brilliant Violet 421™ anti-mouse CD11c
C57BL/6 mouse lymph nodes, fixed O/N in PLP, blocked with 10... C57BL/6 mouse splenocytes were stained with mouse I-A/I-E FI... -
Brilliant Violet 570™ anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E FI... -
Brilliant Violet 785™ anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with I-A/I-E FITC and... -
Brilliant Violet 510™ anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E AP... -
Brilliant Violet 650™ anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E PE... -
Purified anti-mouse CD11c (Maxpar® Ready)
Mouse splenocytes stained with 147Sm-anti-CD45 (30-F11) and ... -
Alexa Fluor® 594 anti-mouse CD11c
C57BL/6 mouse frozen spleen section was fixed with 4% parafo... Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen secti... -
PE/Dazzle™ 594 anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with I-A/I-E FITC and... -
Brilliant Violet 711™ anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with mouse I-A/I-E Pe... -
APC/Fire™ 750 anti-mouse CD11c
C57BL/6 splenocytes were stained with I-A/I-E PE and CD11c (... -
TotalSeq™-A0106 anti-mouse CD11c
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Brilliant Violet 750™ anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
TotalSeq™-B0106 anti-mouse CD11c
-
TotalSeq™-C0106 anti-mouse CD11c
-
KIRAVIA Blue 520™ anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with I-A/I-E APC and ... -
Spark Blue™ 550 anti-mouse CD11c
C57BL/6 mouse splenocytes stained with I-A/I-E (clone M5/114... -
Spark NIR™ 685 anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
Spark UV™ 387 anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
Spark Red™ 718 anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I... -
Spark Blue™ 515 anti-mouse CD11c
C57BL/6 mouse splenocytes were surface stained with anti-mou... -
PerCP/Fire™ 806 anti-mouse CD11c
C57BL/6 splenocytes were stained with anti-mouse I-A/I-E APC... -
Spark PLUS UV™ 395 anti-mouse CD11c
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I...
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