Cell-Vive™ GMP Recombinant Human IL-3 (carrier-free)

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Other Names
Interleukin-3, Burst promoting activity, Eosinophil colony stimulating factor (Eo-CSF), Hematopoietic cell growth factor (HCGF), Mast (MGF/MCGF), Multi-colony stimulating (Multi-CSF), P cell stimulating activity (PCSA), Thy1 inducing factor
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CV_GMP_RECOM_Human_IL3_CF_020524
Recombinant Human IL-3 induces the proliferation of TF-1 human erythroleukemic cells in a dose-dependent manner. The ED50 for this effect is 0.25 – 1.25 ng/mL.
  • CV_GMP_RECOM_Human_IL3_CF_020524
    Recombinant Human IL-3 induces the proliferation of TF-1 human erythroleukemic cells in a dose-dependent manner. The ED50 for this effect is 0.25 – 1.25 ng/mL.
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564314 25 µg 476€
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564316 100 µg 1320€
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Description

Interleukin-3 (lL-3) is a hemopoietic growth factor involved in the survival, proliferation, and differentiation of multipotent hemopoietic cells. In addition to granulocyte-macrophage colony-stimulating factor (GMCSF) and IL-5, IL-3 is the most potent growth factor for basophils. On mature basophils, these three cytokines act through specific receptors mediating adhesion, migration, and releasability. Mast cells express high amounts of IL-3. In the absence of antigen, rapid release of large amounts of IL-3 in an autocrine dependent manner is responsible for mast cell survival. IL-3 plays a vital role in stimulating basophil and mast cell responses to parasite infections. IL-3 has also been implicated in the pathogenesis of several chronic inflammatory diseases, including asthma, atherosclerosis, and neurodegenerative disorders, such as multiple sclerosis. IL-3 stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. IL-3 is expressed in the major embryonic vessels and regulates the survival and proliferation of hematopoietic stem cells in the early stages of embryonic development.

Product Details
Quality Statement

BioLegend Cell-Vive™ GMP Recombinant proteins are manufactured and tested in accordance with USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Ph. Eur. Chapter 5.2.12 in a dedicated GMP facility compliant with ISO 13485:2016. Specifications and processes include:

  • Low endotoxin level (≤ 0.1 EU/μg)
  • Purity (≥ 95% or higher)
  • Bioburden testing
  • Mycoplasma testing
  • Batch-to-batch consistency
  • Vendor qualification
  • Raw material traceability and documentation
  • Documented procedures and employee training
  • Equipment maintenance and monitoring records
  • Lot-specific certificates of analysis
  • Quality audits per ISO 13485:2016
  • QA review of released products
Technical Data Sheet (pdf)

Product Details

Source
Recombinant human IL-3, amino acid sequence containing Ala20-Phe152 (Accession No. NP-000579), was expressed in E.coli. The carboxy-terminus contains a linker-6His tag.
Molecular Mass
The 146 amino acid recombinant protein has a predicted molecular mass of approximately 16.24 kD. The DTT-reduced and non-reduced proteins migrate at approximately 16 kD - 18 kD by SDS-PAGE. The predicted N-terminal amino acid is Ala.
N-terminal
Sequence Analysis
Met-Ala-Pro-Met-Thr-Gln-Thr-Thr-Ser-Leu
Purity
≥ 95%, as determined by Coomassie stained SDS-PAGE
Formulation
0.22 μm filtered protein solution is in PBS, pH 6.5.
Endotoxin Level
Less than or equal to 0.1 EU per μg protein as determined by the LAL method
Residual Host Cell Protein Content
≤ 0.500 ng/μg by ELISA
Concentration
25 μg and 100 μg sizes are bottled at 0.5 mg/mL
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at - 20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 μg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% endotoxin-free BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week or stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Recombinant human IL-3 induces the proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 0.25 – 1.25 ng/mL. The specific activity of Cell-Vive™ GMP Recombinant Human IL-3 (carrier-free) is ≥ 1.6 x 105 IU/mg when compared against the WHO International Standard for human IL-3 (NIBSC code: 91/510).
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Disclaimer

BioLegend Cell-Vive™ GMP Recombinant proteins are for research use only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

Antigen Details

Structure
Monomer
Bioactivity
Measured by its ability to induce the proliferation of TF-1 human erythroleukemic cells.
Cell Sources
Activated T cells, Th1, Th2, mast cells, eosinophils, keratinocytes, NK cells, and endothelial cells
Cell Targets
Erythroid cells, megakaryocytes, neutrophils, eosinophils, basophils, mast cells, monocytic lineages
Receptors
Heterodimeric receptor formed by IL-3Rα (CD123) and a common β subunit (βc) that is shared with the IL-5R and GM-CSFR
Antigen References
  1. Kitamura T, et al. 1989. J Cell Physiol. 140:323-34.
  2. Kohno M, et al. 2005. Blood. 105:2059-65
  3. Murphy JM and Young IG. 2006. Vitam Horm. 74:1-30.
  4. Kleemann R, et al. 2008. Cardiovasc Res. 79:360-76.
  5. Valent P and Dahinden CA. 2010. Curr Opin Hematol. 17:60-6.
  6. Mirza S, et al. 2010. J Biol Chem. 285:2237.
  7. Bénard A, et al. 2021. Nat Commun. 12:1112.
Regulation
IL-3 is a pleiotropic factor which promotes both self-renewal and differentiation of early multipotential progenitors and is involved in inducible hematopoiesis in response to infections.
Gene ID
3562 View all products for this Gene ID
UniProt
View information about IL-3 on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 07/02/2024

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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