- Clone
- 5E5-G5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Histone H3.1, histone H3/a, histone 1, H3/a, H3 histone family, member A
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![5E5-G5_HRP_Histone-H3-Dimethyl_Antibody_1_120219 5E5-G5_HRP_Histone-H3-Dimethyl_Antibody_1_120219](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/5E5-G5_HRP_Histone-H3-Dimethyl_Antibody_1_120219.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Western blot of HRP anti-Histone H3 Dimethyl (Lys9) antibody (5E5-G5). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate, Lane 3: 10 µg of mouse brain lysate, Lane 4: 10 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system. * The predicted molecular weight of dimethyl Histone H3 is at 17 kD. ** The possible homodimers are at 35 kD (Antigen References #1 and #2) -
IHC staining of HRP anti-Histone H3 Dimethyl (Lys9) antibody (clone 5E5-G5) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 420902), the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of HRP anti-Histone H3 Dimethyl (Lys9) antibody (clone 5E5-G5) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 420902), the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of HRP anti-Histone H3 Dimethyl (Lys9) antibody (clone 5E5-G5) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 420902), the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. DAB was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
815504 | 100 µg | 296€ |
Histone H3 is a 17 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. The histones exhibit cell cycle-dependent transcriptional regulation except for the variant H3.3 which is cell cycle-independent. Histone H3 can be modified by phosphorylation, acetylation, ubiquitination, ribosylation, and methylation. Histone H3 has been shown to interact with Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1.
Product DetailsProduct Details
- Verified Reactivity
- Human, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a synthetic peptide from Human Histone H3, di-methylated at Lysine-9.
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.05 - 0.1 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Additional Product Notes
-
For IHC-P application, permeabilization with 0.25 % Triton X-100 is recommended.
- RRID
-
AB_2820126 (BioLegend Cat. No. 815504)
Antigen Details
- Structure
- Expected MW: 17 kD
- Biology Area
- Cell Biology, Neuroscience, Signal Transduction
- Antigen References
-
- Murzina N, et al. 2008. Structure. 16:1077.
- Chen S, et al. 2015. Genes & Dev. 29:1326.
- Gene ID
- 8350 View all products for this Gene ID
- UniProt
- View information about Histone on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Histone Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Histone H3 Dimethyl (Lys9) | 5E5-G5 | WB,IHC-P |
HRP anti-Histone H3 Dimethyl (Lys9) | 5E5-G5 | WB,IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-Histone H3 Dimethyl (Lys9)
Western blot of purified anti-Histone H3 Dimethyl (Lys9) ant... IHC staining of purified anti-Histone H3 Dimethyl (Lys9) ant... IHC staining of purified anti-Histone H3 Dimethyl (Lys9) ant... IHC staining of purified anti-Histone H3 Dimethyl (Lys9) ant... -
HRP anti-Histone H3 Dimethyl (Lys9)
Western blot of HRP anti-Histone H3 Dimethyl (Lys9) antibody... IHC staining of HRP anti-Histone H3 Dimethyl (Lys9) antibody... IHC staining of HRP anti-Histone H3 Dimethyl (Lys9) antibody... IHC staining of HRP anti-Histone H3 Dimethyl (Lys9) antibody...
Follow Us