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Human TruStain FcX™ (Fc Receptor Blocking Solution)

Pricing & Availability
Regulatory Status
RUO
Other Names
Fc Blocker, FcγR blocker, Fc inhibitor
Ave. Rating
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Product Citations
529 publications
A_Human_TruStain_FcX_031110
Human TruStain FcX™ treated (blue) or non-treated (red) THP-1 cells stained with an irrelevant mouse IgG2a PE mAb (above). Dotted histogram represents unstained cells.
  • A_Human_TruStain_FcX_031110
    Human TruStain FcX™ treated (blue) or non-treated (red) THP-1 cells stained with an irrelevant mouse IgG2a PE mAb (above). Dotted histogram represents unstained cells.
  • A_Human_TruStain_FcX_031110
    Human TruStain FcX™ treated (blue) or non-treated (red) THP-1 cells stained with an irrelevant rat IgG2b PE mAb (above). Dotted histogram represents unstained cells.
  • C_Human_TruStain_FcX_031110
    Human TruStain FcX™ treated (filled histograms) or non-treated (open histograms) THP-1 cells stained with anti-human HLA-DR PE (red) or an isotype control (IgG2a PE, blue) mAbs. Note: non-treated cells show false-negative HLA-DR staining due to high binding of mouse IgG2a isotype.
Cat # Size Price Quantity Check Availability Save
422301 50 tests 81€
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422302 200 tests 184€
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Description

Human Fc receptors (FcRs) are expressed on a variety of cells, such as monocytes, granulocytes, B cells and dendritic cells. The cells with FcR expression sometimes give false positive or false negative results of immunofluorescent staining due to the FcRs-mediated Ig Fc binding. Human TruStain FcX™ is specially formulated for blocking the FcR-involved unwanted staining without interfering with antibody-mediated specific staining of human cells. Human TruStain FcX™ is compatible with flow cytometric staining with anti-human CD16 (clone 3G8), CD32 (clone FUN-2), and CD64 (clone 10.1) antibodies.

Product Details
Technical Data Sheet (pdf)

Product Details

Storage & Handling
Store between 2°C and 8°C.
Application

FC - Quality tested
ICFC, ICC - Reported in the literature, not verified in house
Recommended Usage

It is recommended to add 5 µl of Human TruStain FcX™ per million cells in 100 µl staining volume, mix and incubate at room temperature for 5-10 minutes prior to staining with antibody of interest. It is not necessary to wash cells between these blocking and immunostaining steps.

Application Notes

This buffer contains specialized human IgG in PBS containing 0.09% sodium azide. It is not recommended to be used for staining human IgG. Handle as biohazard agent.

Application References
  1. de la Cruz J, et al. 2011. J. Immunol. 186:2282. PubMed
  2. Luo L, et al. 2011. Exp Mol Pathol. 91:596. PubMed
  3. Szerlip NJ, et al. 2012. PNAS. 109:3041. PubMed
  4. Bromley SK, et al. 2013. J. Immunol. 190:970. PubMed
  5. Trujilo G, et al. 2013. J. Immunol. 191:848. PubMed
  6. Edin S, et al. 2013. PLoS One. 18:74982. PubMed
  7. Euzebio Alves VT, et al. 2013. Infect Immun. 81:4399. PubMed
  8. Borg ZD, et al. 2014. J. Immunol. 192:189. PubMed
  9. Klingenberg R, et al. 2014. Eur Heart J. PubMed
  10. Lines JL, et al. 2014. Cancer Res. 74:1924. PubMed
  11. Decker D, et al. 2014. Lupus. 23:802. PubMed
  12. Jitschin R, et al. 2014. Blood. 123:2663. PubMed
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Product Citations
  1. Palacios-Arreola M, et al. 2017. Sci Rep. 10.1038/s41598-017-10135-1. PubMed
  2. Chen X, et al. 2019. Exp Ther Med. 1.265972222. PubMed
  3. Fisher RC, et al. 2019. Neoplasia. 1.061805556. PubMed
  4. Winkler CW, et al. 2019. J Neuroinflammation. 0.825694444. PubMed
  5. O'Huallachain M, et al. 2020. Commun Biol. 3:213. PubMed
  6. Klingenberg R, et al. 2014. Eur Heart J. . PubMed
  7. Fujisawa D, et al. 2014. J Allergy Clin Immunol. 134:622. PubMed
  8. Simon A, et al. 2015. Blood. 126: 378 - 385. PubMed
  9. Mitton B, et al. 2016. Leukemia. 10.1038/leu.2016.139. PubMed
  10. Wanke F, et al. 2017. Cell Rep. 18(5):1270-1284. PubMed
  11. Ecker JW, et al. 2020. Vaccines (Basel). 8:00. PubMed
  12. Liu C, et al. 2021. Cell. 184(7):1836-1857.e22. PubMed
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RRID
AB_2818986 (BioLegend Cat. No. 422302)

Antigen Details

Molecular Family
Fc Receptors
Gene ID
NA

Related FAQs

Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?

Yes

What is the concentration of Human TruStain FcX™ (Fc receptor blocking solution, Cat#422301)?
This information is proprietary.
Go To Top Version: 4    Revision Date: 07/14/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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