- Clone
- QA17A36 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Sca-1
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![QA17A36_PE_Ly-6A-E_RECOM_Antibody_1_030822_updated QA17A36_PE_Ly-6A-E_RECOM_Antibody_1_030822_updated](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/QA17A36_PE_Ly-6A-E_RECOM_Antibody_1_030822_updated.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with anti-mouse Ly-6A/E (Sca-1) recombinant (clone QA17A36) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram). -
C57BL/6 mouse bone marrow were stained with anti-mouse Ly-6A/E (Sca-1) recombinant (clone QA17A36) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram). Data was gated on the lymphoid population.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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163803 | 25 µg | 88€ | ||||
163804 | 100 µg | 212€ |
Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Recombinant
- Host Species
- Rat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- RRID
-
AB_2922499 (BioLegend Cat. No. 163803)
Antigen Details
- Structure
- Ly-6 multigene family, 18 kD
- Distribution
-
Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells
- Function
- Regulates B and T cell responses
- Antigen References
-
- Upadhyay G. 2019. Front Immunol. 10:819.
- Morrison SJ, et al. 1994. Immunity. 1:661.
- Spangrude GJ, et al. 1988. J Immunol. 141:3697.
- Gene ID
- 110454 View all products for this Gene ID
- UniProt
- View information about Ly-6A/E on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All Ly-6A/E Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse Ly-6A/E (Sca-1) Recombinant Antibody | QA17A36 | FC |
PE anti-mouse Ly-6A/E (Sca-1) Recombinant Antibody | QA17A36 | FC |
APC anti-mouse Ly-6A/E (Sca-1) Recombinant Antibody | QA17A36 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse Ly-6A/E (Sca-1) Recombinant Antibody
C57BL/6 mouse splenocytes were stained with purified Ly-6A/E... -
PE anti-mouse Ly-6A/E (Sca-1) Recombinant Antibody
C57BL/6 mouse splenocytes were stained with anti-mouse Ly-6A... C57BL/6 mouse bone marrow were stained with anti-mouse Ly-6A... -
APC anti-mouse Ly-6A/E (Sca-1) Recombinant Antibody
C57BL/6 mouse bone marrow were stained with anti-mouse CD117...
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