Purified anti-ApoER2 (LRP8) Antibody

Pricing & Availability
Clone
25G5 (See other available formats)
Other Names
LRP8, APOER2, LDL Receptor Related Protein 8
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
25G5_Purified_ApoER2_Antibody_040219.png
Western blot of purified anti-ApoER2 (LRP8) antibody (clone 25G5). Lanes 1, 5: Molecular weight marker; Lanes 2, 6: 40 µg of mouse brain lysate; Lanes 3, 7: 40 µg of rat brain lysate; Lanes 4, 8: 20 µg of SH-SY5Y cell lysate. The blots were incubated with 2 µg/mL of the primary antibody or Rat IgG2a isotype control overnight at 4°C, followed by incubation with HRP-labeled goat anti-rat IgG (Cat. No. 405405). Enhanced chemiluminescence was used as the detection system.
  • 25G5_Purified_ApoER2_Antibody_040219.png
    Western blot of purified anti-ApoER2 (LRP8) antibody (clone 25G5). Lanes 1, 5: Molecular weight marker; Lanes 2, 6: 40 µg of mouse brain lysate; Lanes 3, 7: 40 µg of rat brain lysate; Lanes 4, 8: 20 µg of SH-SY5Y cell lysate. The blots were incubated with 2 µg/mL of the primary antibody or Rat IgG2a isotype control overnight at 4°C, followed by incubation with HRP-labeled goat anti-rat IgG (Cat. No. 405405). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
862101 25 µg 72€
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862102 100 µg 188€
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Description

LDL Receptor Related Protein 8 (LRP8), also known as ApoER2, is a receptor for members of low-density lipoproteins. LRP8 interacts with its ligand reelin, and triggers reelin signaling to regulate cellular processes such as neuronal cell migration and long-term potentiation of mature neurons. LRP8 also interacts with and downregulates APP endocytosis. This interactions leads to increased amyloid beta production, demonstrating a major role for LRP8 in the pathogenesis of  Alzheimer’s disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2801239 (BioLegend Cat. No. 862101)
AB_2801240 (BioLegend Cat. No. 862102)

Antigen Details

Structure
LRP8 is a 963 amino acid protein with an apparent molecular mass of ~105 kD.
Distribution

Tissue Distribution: brain, endocrine tissue, testis, and placenta
Cellular Distribution: plasma membrane, extracellular, and cytoskeleton

Function
LRP8 functions as the receptor for reelin. Binding and endocytosis of reelin triggers downstream signaling pathways that affects neuronal migration and postnatal LTP.
Interaction
FE65, JIP1, JIP2, Thrombosphondin, F-Spondin
Cell Type
Neurons
Biology Area
Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family
Apolipoproteins
Antigen References
  1. Santana J and Marzolo MP. 2017. Biochem J. 474(18):3137
  2. Kim J, et al. 2014. Mol Cells. 37(11):767
  3. Lara-Castillo N and Johnson ML. 2015. Rev Endocr Metab Disord. 16(2):141
Gene ID
7804 View all products for this Gene ID 16975 View all products for this Gene ID
UniProt
View information about ApoER2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/02/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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