- Clone
- R24 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- GD3S, SIAT8, Alpha-N-acetylneuraminide alpha-2,8-sialyltransferase, SIAT8-A, ganglioside GD3 synthase, sialytransferase St8Sia I, alpha-2,8-sialyltransferase 8A, disialoganglioside (GD3) synthase, ganglioside-specific alpha-2,8-polysialyltransferase
- Previously
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Signet Catalog# 8110-01
Covance Catalog# SIG-38110
- Isotype
- Mouse IgG3, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![R24_Purified_MEL-1_Antibody_IHC_032615 R24_Purified_MEL-1_Antibody_IHC_032615](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/R24_Purified_MEL-1_Antibody_IHC_032615.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Staining of MEL-1 (R24) on frozen human melanoma tissue.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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917701 | 1 mL | 314€ |
GD3, also known as MEL-1, is a ganglioside found in almost all melanomas and is an important biomarker for malignant melanoma. GD3 is found in melanocytes and other cells that originate in the neuroectoderm. Anti-GD3 antibodies have been shown to induce partial remission of tumor growth due to the proliferation of cytotoxic lymphocytes.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was raised against SK-MEL-28 melanoma cell line.
- Formulation
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Phosphate-buffered solution with BSA + 0.09% NaN3.
Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service. - Preparation
- The antibody was purified by affinity chromatography.
- Storage & Handling
- Store between 2°C and 8°C.
- Application
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IHC-F - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunohistochemical staining.
The optimal working dilution should be determined for each specific assay condition.
• IHC: ≥1:40 with for Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).
Tissue Sections: The preferred specimens for this antibody are frozen tissues and/or cytocentrifuge preparations fixed 10 minutes in 4°C acetone.
Pretreatment: Not required
Incubation: 20-60 minutes at room temperature - Application Notes
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This antibody is effective in immunohistochemistry (IHC).
Positive tissue (human): Melanoma
MEL-1 (clone R24) recognizes disialoganglioside GD3, a prominent cell surface marker of human melanoma cells. - Application References
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- Meidenbauer N, et al. 2004. Cancer Res. 64: 6319.
- Kasahara K, et al. 2000. J. Biol. Chem. 275: 34701.
- Watarai S, et al. 1991. J. Biochem. 110: 889.
- Yuyama K, et al. 2007. J. Biol. Chem. 282: 26392.
- Product Citations
-
- RRID
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AB_2565200 (BioLegend Cat. No. 917701)
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All GD3 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-GD3 (MEL-1) | R24 | IHC-F |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-GD3 (MEL-1)
Staining of MEL-1 (R24) on frozen human melanoma tissue.
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