- Clone
- HMH4-5G1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- B7H4, B7S1, B7-S1, B7x
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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139409 | 100 µg | 216€ | ||||
139410 | 1 mg | 542€ |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
B7-H4 is a newly discovered B7 family member that negatively regulates T cell immunity. In vitro, B7-H4 inhibits CD4+ and CD8+ T cell proliferation, cytokine production, and generation of alloreactive cytotoxic T-cells (CTLs). In vivo, blockage of endogenous B7-H4 by specific monoclonal antibodies promotes T cell responses. A new reported function of B7-H4 is as an important negative regulator of innate immunity through growth inhibition of neutrophils. It has been reported that B7-H4 is not constitutively expressed on peripheral tissues but can be induced to express on T cells, B cells, macrophages, and dendritic cells. B7-H4 is expressed on some tumor cancer cells. The role of B7-H4 in tumor progression may be to transform precancerous cells and then protect them from immunosurveillance. Although B- and T-lymphocyte attenuator (BTLA) was proposed to be the receptor for B7-H4, further studies are needed to identify the inhibitory receptor of B7-H4.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse B7-H4 human IgG1 Fc fusion protein
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2832506 (BioLegend Cat. No. 139409)
AB_2832507 (BioLegend Cat. No. 139410)
Antigen Details
- Structure
- Belongs to the B7 family, contains two extracellular Ig-like domains.
- Distribution
-
Induced to express on T cells, B cells, macrophages, and dendritic cells. It is also expressed on some tumor cancer cells.
- Function
- Inhibit T cell activation, cell cycle progression, and cytokine production.
- Cell Type
- B cells, Dendritic cells, Macrophages, T cells
- Biology Area
- Costimulatory Molecules, Immunology, Innate Immunity
- Antigen References
-
1. Choi IH, et al. 2003. J. Immunol. 171:4650.
2. Prasad DV, et al. 2003. Immunity 18:863.
3. Kryczek I, et al. 2006. J. Exp. Med. 203:871. - Gene ID
- 242122 View all products for this Gene ID
- UniProt
- View information about B7-H4 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All B7-H4 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-mouse B7-H4 (B7S1, B7X) | HMH4-5G1 | FC |
APC anti-mouse B7-H4 (B7S1, B7X) | HMH4-5G1 | FC |
Ultra-LEAF™ Purified anti-mouse B7-H4 (B7S1, B7X) | HMH4-5G1 | FC |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
PE anti-mouse B7-H4 (B7S1, B7X)
Mouse B7-H4 transfected 293 cells stained with HMH4-5G1 PE -
APC anti-mouse B7-H4 (B7S1, B7X)
B7-H4 transfected 293 cells were stained with B7-H4 (clone H... -
Ultra-LEAF™ Purified anti-mouse B7-H4 (B7S1, B7X)
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