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APC/Cyanine7 anti-human CD19 Antibody

Pricing & Availability
Clone
HIB19 (See other available formats)
Regulatory Status
RUO
Workshop
V CD19.11
Other Names
B4
Isotype
Mouse IgG1, κ
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Product Citations
publications
HIB19_APCCyanine7_CD19_Antibody_032019_updated.png
Human peripheral blood lymphocytes were stained with CD20 FITC and CD19 (clone HIB19) APC/Cyanine7 (left) or Mouse IgG1, κ isotype control (right)
  • HIB19_APCCyanine7_CD19_Antibody_032019_updated.png
    Human peripheral blood lymphocytes were stained with CD20 FITC and CD19 (clone HIB19) APC/Cyanine7 (left) or Mouse IgG1, κ isotype control (right)
See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
302217 25 tests 123€
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302218 100 tests 249€
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Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD19 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

 

Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing

Additional Product Notes

BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
  4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
  5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
  6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Shoda H, et al. 2017. Arthritis Research & Therapy . 10.1186/s13075-017-1308-y. PubMed
  2. Sander J et al. 2017. Immunity. 47(6):1051-1066 . PubMed
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  4. Neff CP et al. 2018. EBioMedicine. 30:192-202 . PubMed
  5. Yamauchi T et al. 2018. Cancer cell. 33(3):386-400 . PubMed
  6. Novakova L et al. 2018. Journal of neurochemistry. 146(3):322-332 . PubMed
  7. Shehata L, et al. 2019. Nat Commun. 10:1126. PubMed
  8. Körner C et al. 2017. Cell host & microbe. 22(1):111-119 . PubMed
  9. Tomellini E, et al. 2020. Cell Reports. 28(4):1063-1073.e5.. PubMed
  10. Suzuki D, et al. 2020. Stem Cell Reports. 14:49. PubMed
  11. Martín–Gayo E, et al. 2020. Cell Rep. 30:984. PubMed
  12. Shehata L, et al. 2019. Cell Rep. 28:3300. PubMed
  13. Zhou Y, et al. 2017. Front Cell Infect Microbiol. 7:457. PubMed
  14. Samarani S, et al. 2020. Mediators Inflamm. 2020:6401969. PubMed
  15. Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed
  16. Urlaub D, et al. 2019. Arthritis Res Ther. 1.067361111. PubMed
  17. Joseph A, et al. 2010. J Virol. 84:6645. PubMed
  18. Simpfendorfer K, et al. 2012. Hum Mol Genet. 21:3918. PubMed
  19. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  20. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  21. Zhang J, et al. 2015. J Biol Chem. 290 19093 . PubMed
  22. Kuebler P, et al. 2015. Proc Natl Acad Sci U S A. 112: 8379 - 8384. PubMed
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  24. Deng C, et al. 2016. Diabetes Care. 39: 434 - 440. PubMed
  25. Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed
  26. Nagafuchi Y, et al. 2016. Sci Rep. 6:29338. PubMed
  27. AC Belkina, JE Snyder-Cappione 2017. Cytometry A. 91:175-179. PubMed
  28. Tsuchida Y, et al. 2017. PLoS One. 10.1371/journal.pone.0169646. PubMed
  29. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  30. James KR, et al. 2020. Nat Immunol. 1.113194444. PubMed
  31. Yu B, et al. 2021. Cell. 184(7):1790-1803.e17. PubMed
  32. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  33. Herati RS, et al. 2021. Cell Reports Medicine. 2(5):100262. PubMed
  34. Pan YG, et al. 2021. Immunity. 54(6):1245-1256.e5. PubMed
  35. Kreutmair S, et al. 2021. Immunity. . PubMed
  36. Punik J, et al. 2021. Cell Reports. 35(13):109320. PubMed
  37. Woo MS, et al. 2021. iScience. 24(7):102752. PubMed
  38. Sinha D, et al. 2021. J Immunother Cancer. 9:. PubMed
  39. Sananez I, et al. 2021. EBioMedicine. 72:103615. PubMed
  40. Golovkin A, et al. 2021. Viruses. 13:. PubMed
  41. Sitlinger A, et al. 2021. Cells. 10:. PubMed
  42. Pozzetto B, et al. 2021. Nature. 600:701. PubMed
  43. Frensch M, et al. 2021. Cell Mol Life Sci. 78:8165. PubMed
  44. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  45. Valestrand L, et al. 2022. Am J Pathol. . PubMed
  46. Liu W, et al. 2022. Bio Protoc. 12:e4285. PubMed
  47. Pahl MC, et al. 2022. Genome Biol. 23:125. PubMed
  48. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  49. Li Y, et al. 2021. Nat Commun. 12:87. PubMed
  50. Zhang X, et al. 2021. Front Immunol. 12:680068. PubMed
  51. Sicklinger F, et al. 2021. J Clin Invest. 131:. PubMed
  52. Arcangeli S, et al. 2022. J Clin Invest. 132: . PubMed
  53. Andonian BJ, et al. 2022. Sci Rep. 12:7450. PubMed
  54. Kudryavtsev IV, et al. 2021. Curr Issues Mol Biol. 44:194. PubMed
  55. Hui Z, et al. 2022. Cell Death Dis. 13:607. PubMed
  56. Shemesh A, et al. 2022. J Exp Med. 219:. PubMed
  57. Coelho CH, et al. 2020. JCI Insight. 5:. PubMed
  58. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  59. Pan YG, et al. 2022. Methods Mol Biol. 2574:31. PubMed
  60. Wang H, et al. 2022. iScience. 25:105065. PubMed
  61. Bianchi A, et al. 2022. Genome Biol. 23:229. PubMed
  62. Sefik E, et al. 2022. Nature. 606:585. PubMed
  63. Zhao Y, et al. 2022. Front Immunol. 13:1041176. PubMed
  64. Pušnik J, et al. 2023. Nat Commun. 14:572. PubMed
  65. Souter MNT, et al. 2022. J Exp Med. 219:. PubMed
  66. Bayik D, et al. 2022. Cancer Res. 82:4274. PubMed
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RRID
AB_314247 (BioLegend Cat. No. 302217)
AB_314248 (BioLegend Cat. No. 302218)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

B lineage (except plasma cells), follicular dendritic cells

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 03/20/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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