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Brilliant Violet 510™ anti-human CD56 (NCAM) Antibody

Pricing & Availability
Clone
HCD56 (See other available formats)
Regulatory Status
RUO
Other Names
Leu-19, NKH1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
HCD56_BV510_CD56_Antibody_FC_082512
Human peripheral blood lymphocytes were stained with CD3 APC and CD56 (clone HCD56) Brilliant Violet 510™.
  • HCD56_BV510_CD56_Antibody_FC_082512
    Human peripheral blood lymphocytes were stained with CD3 APC and CD56 (clone HCD56) Brilliant Violet 510™.
See Brilliant Violet 510™ spectral data
Cat # Size Price Quantity Check Availability Save
318339 25 tests 172€
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318340 100 tests 326€
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Description

CD56 is a single transmembrane glycoprotein also known as NCAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone HCD56 is not recommended for immunohistochemistry formalin-fixed paraffin-embedded tissue.

Application References
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations
  1. Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed
  2. Abd Hamid M et al. 2019. Cancer Immunol Res. 7(8):1293-1306 . PubMed
  3. Rölle A, et al. 2018. Cell Rep. 24:1967. PubMed
  4. Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed
  5. Serra EG, et al. 2020. Nat Commun. 11:995. PubMed
  6. Abd Hamid M, et al. 2020. Cancer Immunology Research. 8(2):203-216. PubMed
  7. Evans RDR, et al. 2020. Nat Commun. 3.491666667. PubMed
  8. Maas RJ, et al. 2020. Oncoimmunology. 9:1843247. PubMed
  9. Jiang Y, et al. 2020. Clin Immunol. 218:108516. PubMed
  10. Sutton HJ, et al. 2021. Cell Reports. 34(6):108684. PubMed
  11. Townsley SM, et al. 2021. Cell Host Microbe. 29(4):564-578.e9. PubMed
  12. Nel I, et al. 2021. Diabetologia. 64(10):2306-2321. PubMed
  13. Townsley SM, et al. 2021. STAR Protocols. 2(3):100771. PubMed
  14. Van der Meer JM, et al. 2020. Cancer Immunol Immunother. . PubMed
  15. Tan J, et al. 2021. Sci Transl Med. 13: . PubMed
  16. Cohen CA, et al. 2021. Nat Commun. 12:4678. PubMed
  17. Wang L, et al. 2021. Science. 373:. PubMed
  18. Cho H, et al. 2021. Sci Transl Med. 13:eabj5413. PubMed
  19. Yang Zhou J, et al. 2022. Front Immunol. 13:898473. PubMed
  20. Port JR, et al. 2020. J Virol. 94:. PubMed
  21. Van der Meer JMR, et al. 2021. Oncoimmunology. 10:1981049. PubMed
  22. Wang H, et al. 2022. Front Immunol. 13:852436. PubMed
  23. Wang H, et al. 2022. iScience. 25:105065. PubMed
  24. Dacon C, et al. 2022. Cell Host Microbe. Online ahead of print. PubMed
  25. Lima NS, et al. 2022. Nat Commun. 13:7733. PubMed
RRID
AB_2561385 (BioLegend Cat. No. 318339)
AB_2561944 (BioLegend Cat. No. 318340)

Antigen Details

Structure
Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution

NK cells, T subset, neural tissue, some LGL and myeloid leukemias

Function
Adhesion
Ligand/Receptor
Heparin sulfate
Cell Type
B cells, Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Lanier L, et al. 1991. J. Immunol. 146:4421.
2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71.
3. Cremer H, et al. 1994. Nature 367:455.

Gene ID
4684 View all products for this Gene ID
UniProt
View information about CD56 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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