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Brilliant Violet 650™ anti-human CD20 Antibody

Pricing & Availability
Clone
2H7 (See other available formats)
Regulatory Status
RUO
Workshop
IV B201
Other Names
B1, Bp35
Isotype
Mouse IgG2b, κ
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Product Citations
publications
2H7_BV650_052312
Human peripheral blood lymphocytes were stained with CD20 (clone 2H7) Brilliant Violet 650™.
  • 2H7_BV650_052312
    Human peripheral blood lymphocytes were stained with CD20 (clone 2H7) Brilliant Violet 650™.
See Brilliant Violet 650™ spectral data
Cat # Size Price Quantity Check Availability Save
302335 25 tests 173€
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302336 100 tests 317€
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Description

CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human tonsillar B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The epitope recognized by clone 2H7 has been mapped to the sequence YNCEPANPSEKNSPST which lies in the large extracellular loop of human CD20. Additional reported applications (for the relevant formats) include: immunoprecipitation4 and immunohistochemical staining of acetone-fixed frozen sections5.

Application References
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.
  4. Polyak MJ, et al. 2002. Blood 99:3256. (IP)
  5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
Product Citations
  1. Friedensohn S, et al. 2018. Front Immunol. 9:1401. PubMed
  2. Ferrando-Martinez S, et al. 2018. J Clin Invest. 128:2089. PubMed
  3. Cirelli KM et al. 2019. Cell. 177(5):1153-1171 . PubMed
  4. Meehan S, et al. 2019. Commun Biol. 2:229. PubMed
  5. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  6. Eckard AR, et al. 2017. Antivir Ther. 1.38125. PubMed
  7. Eckard AR, et al. 2017. Antivir Ther. 1.177083333. PubMed
  8. Lindner JM, et al. 2019. Immunity. 50:668. PubMed
  9. Hagan T, et al. 2020. Cell. 178(6):1313-1328.e13.. PubMed
  10. Chowdhury A, et al. 2015. J Immunol. 195: 3237 - 3247. PubMed
  11. Roberts E, et al. 2016. PLoS One. 11:e0168488. PubMed
  12. Geetha H. Mylvaganam, Daniel Rios 2017. Proc Natl Acad Sci U S A. 114(8):1976-1981. PubMed
  13. Rydyznski Moderbacher C, et al. 2020. Cell. 183(4):996-1012.e19. PubMed
  14. Buggert M, et al. 2020. Cell. 183(7):1946-1961.e15. PubMed
  15. Ramaswamy A, et al. 2021. Immunity. 54(5):1083-1095.e7. PubMed
  16. Williams WB, et al. 2021. Cell. 184(11):2955-2972.e25. PubMed
  17. Mulder K, et al. 2021. Immunity. 54(8):1883-1900.e5. PubMed
  18. Zabaleta N, et al. 2021. Cell Host Microbe. :. PubMed
  19. Charles TP, et al. 2021. PLoS Pathog. 17:e1009257. PubMed
  20. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  21. Cillo AR, et al. 2021. Cell Rep Med. 2:100476. PubMed
  22. Weskamm LM, et al. 2022. Cell Rep Med. 3:100685. PubMed
  23. Xu C, et al. 2022. iScience. 25:105123. PubMed
  24. Weskamm LM, et al. 2022. STAR Protoc. 3:101902. PubMed
  25. Hastie KM, et al. 2023. Cell Rep. 42:112421. PubMed
RRID
AB_11218609 (BioLegend Cat. No. 302335)
AB_2563806 (BioLegend Cat. No. 302336)

Antigen Details

Structure
Four transmembrane protein (TM4SF), heavily phosphorylated after activation, 33-37 kD
Distribution

B cell, T cell subsets

Function
B cell activation
Ligand/Receptor
Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
Cell Type
B cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Hultin L, et al. 1993. Cytometry 14:196.
2. Tedder T, et al. 1994. Immunol. Today 15:450.

Gene ID
931 View all products for this Gene ID
UniProt
View information about CD20 on UniProt.org

Related FAQs

What is the difference between two anti human CD20 clones 2H7 and 1412?

For clone 1412: This clone specifically recognizes cytoplasmic domain of CD20 and thus can only be used for intracellular flow cytometry. In this instance you will need to include the fixation and permeabilization steps. Please follow the intracellular flow cytometry staining protocol.

For clone 2H7: This clone is ok for regular surface staining for CD20 and there is no need for any fixation and permeabilization steps.

Our technical protocols can be found here.

Go To Top Version: 3    Revision Date: 10/08/2013

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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