Loading...

Brilliant Violet 650™ anti-mouse/human CD11b Antibody

Pricing & Availability
Clone
M1/70 (See other available formats)
Regulatory Status
RUO
Other Names
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
M1slash70_BV650_020212
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 650™. Data shown was gated on myeloid cell population.
  • M1slash70_BV650_020212
    C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 650™. Data shown was gated on myeloid cell population.
See Brilliant Violet 650™ spectral data
Cat # Size Price Quantity Check Availability Save
101239 125 µL 168€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
101259 50 µg 219€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse,Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.3 µg per million cells in 100 µl volume. For flow cytometric staining using the test size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F).

Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4, in vitro blocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, immunohistochemistry of acetone-fixed frozen sections5,11-13, and spatial biology (IBEX)35,36. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 101248).

Application References
  1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP)
  2. Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete)
  3. Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block)
  4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP)
  5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
  6. Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF)
  7. Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF)
  8. D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete)
  9. Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block)
  10. Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF)
  11. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  12. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC)
  13. Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC)
  14. Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC)
  15. Olver S, et al. 2006. Cancer Research 66:571. (FC)
  16. Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed
  17. Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC)
  18. Dzhagalov I, et al. 2007. Blood 109:1620. (FC)
  19. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  20. Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed
  21. Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed
  22. Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:1894. PubMed
  23. Kim DD, et al. 2008. Blood 112:1109. PubMed
  24. Guo Y, et al. 2008. Blood 112:480. PubMed
  25. Norian LA, et al. 2009. Cancer Res. 69:3086. (FC) PubMed
  26. Baumgartner CK, et al. 2010. J. Immunol. 184:573. PubMed
  27. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  28. Whiteland J, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
  29. Weber GF, et al. 2014. J Exp Med. 211:1243. PubMed
  30. Ashok A, et al. 2015. Toxicol Sci. 143:64. PubMed
  31. Price PJ, et al. 2015. J Immunol. 194:1164. PubMed
  32. Doni A, et al. 2015. J Exp Med. 212:905. PubMed
  33. Ferreira R, et al. 2016. J Infect Dis. 213: 669 - 673. PubMed
  34. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  35. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  36. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Thion MS et al. 2018. Cell. 172(3):500-516 . PubMed
  2. Costa A et al. 2018. Cancer cell. 33(3):463-479 . PubMed
  3. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  4. Teo TH, et al. 2018. Nat Commun. 9:3905. PubMed
  5. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
  6. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
  7. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  8. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  9. Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed
  10. Su W et al. 2019. Cancer Cell. 36(2):139-155 . PubMed
  11. Li H, et al. 2019. Cell. 176:775. PubMed
  12. Zhu Y et al. 2017. The Journal of Neuroscience. 37(9):2362-2376 . PubMed
  13. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  14. Gimblet C et al. 2017. Cell host & microbe. 22(1):13-24 . PubMed
  15. Sierro F, et al. 2017. Immunity. 47:374. PubMed
  16. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  17. Bittner–Eddy PD, et al. 2017. Front Immunol. 1.304166667. PubMed
  18. O'Boyle C, et al. 2020. Int J Stroke. 0.746527778. PubMed
  19. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  20. Blagih J, et al. 2020. Cell Rep. 30:481. PubMed
  21. Rosenbaum SR, et al. 2020. Cell Rep. 30:510. PubMed
  22. Maniati E, et al. 2020. Cell Rep. 30:525. PubMed
  23. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
  24. Demircioglu F, et al. 2020. Nat Commun. 11:1290. PubMed
  25. Benechet AP, et al. 2019. Nature. 574:200. PubMed
  26. Riedel R, et al. 2020. Nat Commun. 11:2570. PubMed
  27. Tuit S, et al. 2019. Cell Rep. 29:1221. PubMed
  28. Yu YR, et al. 2020. STAR Protocols. 11(3):e0150606. PubMed
  29. Hagan T, et al. 2020. Cell. 178(6):1313-1328.e13.. PubMed
  30. Li Y, et al. 2020. Cell Reports. 27(10):2809-2816.e3.. PubMed
  31. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
  32. Chen YG, et al. 2020. Molecular Cell. 76(1):96-109. PubMed
  33. Lees JG, et al. 2020. PLoS One. 15:e0238164. PubMed
  34. Liu Z, et al. 2020. STAR Protoc. 1:100029. PubMed
  35. Moreno M et al. 2014. The Journal of Neuroscience. 34(24):8175-85 . PubMed
  36. Wiesner D, et al. 2015. PLoS Pathog. 11:1004701. PubMed
  37. Colliou N, et al. 2015. Toxins. 7: 3805 - 3817. PubMed
  38. Brestoff JR, et al. 2021. Cell Metab. 33:270. PubMed
  39. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  40. Grubman A, et al. 2020. Stem Cell Reports. 1.290277778. PubMed
  41. Gniadek TJ, et al. 2020. J Immunother. 43:217. PubMed
  42. BenYoucef A, et al. 2020. J Allergy Clin Immunol. 145:1690. PubMed
  43. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  44. Marn Franco JL, et al. 2020. Cell Reports. 33(13):108547. PubMed
  45. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  46. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  47. Shreeve N, et al. 2021. Immunity. 54(6):1231-1244.e4. PubMed
  48. Dai X, et al. 2021. Molecular Cell. 81(11):2317-2331.e6. PubMed
  49. Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed
  50. De Simone G, et al. 2021. Immunity. :. PubMed
  51. Blriot C, et al. 2021. Immunity. :. PubMed
  52. Özcan A, et al. 2020. J Invest Dermatol. 1003:140. PubMed
  53. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  54. Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed
  55. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  56. Tritz ZP, et al. 2020. J Immunol. 205:1228. PubMed
  57. Tan X, et al. 2021. Sci Adv. 7: . PubMed
  58. Bagayoko S, et al. 2021. PLoS Pathog. 17:e1009927. PubMed
  59. Andreata F, et al. 2021. STAR Protoc. 2:100831. PubMed
  60. Matsushita Y, et al. 2021. J Bone Miner Res. 36:1145. PubMed
  61. Terlizzi M, et al. 2021. Cell Physiol Biochem. 55:539. PubMed
  62. Lin J, et al. 2021. Nat Commun. 12:5321. PubMed
  63. Shen XR, et al. 2021. Viruses. 13:. PubMed
  64. Warheit-Niemi HI, et al. 2022. JCI Insight. 7:. PubMed
  65. Chakraborty S, et al. 2022. Sci Transl Med. . PubMed
  66. Lee A, et al. 2022. Nat Commun. 13:549. PubMed
  67. Abdelfattah N, et al. 2022. Nat Commun. 13:767. PubMed
  68. Taniguchi H, et al. 2022. Cell Rep. 39:110814. PubMed
  69. Hulsebus HJ, et al. 2022. Front Immunol. 13:884719. PubMed
  70. Grigoryan L, et al. 2022. NPJ Vaccines. 7:55. PubMed
  71. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  72. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  73. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  74. Uceda-Castro R, et al. 2022. Cell Rep Med. 3:100821. PubMed
  75. Cautivo KM, et al. 2022. Immunity. 55:254. PubMed
  76. Liu H, et al. 2022. Cancer Immunol Res. 10:1028. PubMed
  77. Brigo N, et al. 2022. Bio Protoc. 12:e4440. PubMed
  78. Sieow JL, et al. 2023. Int J Mol Sci. 24: . PubMed
  79. Kang J, et al. 2023. JCI Insight. 8: . PubMed
  80. Bajaj R, et al. 2022. Cell Rep. 40:111429. PubMed
  81. Christian DA, et al. 2022. Sci Immunol. 7:eabq7432. PubMed
  82. Brigo N, et al. 2023. Cells. 12:. PubMed
  83. Liu J, et al. 2023. Nat Commun. 14:2806. PubMed
  84. Zengel J, et al. 2023. Nat Methods. . PubMed
RRID
AB_11125575 (BioLegend Cat. No. 101239)
AB_2566568 (BioLegend Cat. No. 101259)

Antigen Details

Structure
Integrin family, associates with integrin β2 (CD18), 170 kD
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells

Function
Adhesion, chemotaxis
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Coxon A, et al. 1996. Immunity 5:653.

Gene ID
16409 View all products for this Gene ID 3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org
Go To Top Version: 2    Revision Date: 01/26/2016

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account