Loading...

PE anti-human CD62P (P-Selectin) Antibody

Pricing & Availability
Clone
AK4 (See other available formats)
Regulatory Status
RUO
Workshop
VI P-44
Other Names
GMP-140, PADGEM
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
AK4_PE
Thrombin-activated human platelets stained with AK4 PE
  • AK4_PE
    Thrombin-activated human platelets stained with AK4 PE
See PE spectral data
Cat # Size Price Quantity Check Availability Save
304905 25 tests 86€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
304906 100 tests 190€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD62P is a 140 kD type I transmembrane glycoprotein also known as P-selectin, platelet activation-dependent granule membrane protein (PADGEM), and GMP-140. It is expressed on activated platelets, megakaryocytes, and endothelial cells. CD62P is primarily stored in secretory α-granules in platelets and Weibel-Palade bodies in endothelial cells, and is rapidly relocated to the plasma membrane upon activation. The ligands for CD62P are CD162 and CD24. A primary function of CD62P is cell adhesion during neutrophil rolling, and platelet-neutrophil and platelet-monocyte interactions.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4 and in vitro blocking of adhesion of platelets1. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 304947 & 304948).

Application References
  1. Skinner M, et al. 1991. J. Biol. Chem. 266:5371. (Block)
  2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  3. Yen YT, et al. 2006. J. Virol. 80:2684.
  4. Sato Y, et al. 2005. Blood 106:428. (IHC)
Product Citations
  1. Best MG et al. 2017. Cancer cell. 32(2):238-252 . PubMed
  2. Ye Z, et al. 2019. Mediators Inflamm. 2019:8709583. PubMed
  3. Sung PS, et al. 2019. Nat Commun. 10:2402. PubMed
  4. Karampini E, et al. 2019. Haematologica. 104:2091. PubMed
  5. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  6. Zhang S, et al. 2012. PLoS One. 7:e40641. PubMed
  7. Welsh J, et al. 2011. Br J Haematol. 154:122. PubMed
  8. Crooks M, et al. 2014. PLoS One. 9:111347. PubMed
  9. Artemenko E, et al. 2016. Biochem J. 473: 435 - 448. PubMed
  10. Deng W, et al. 2016. Nat Commun. 7: 12863. PubMed
  11. Bennett JA, et al. 2020. Nat Commun. 2.874305556. PubMed
  12. Machata S, et al. 2021. Front Immunol. 11:565869. PubMed
  13. Slingsby MHL, et al. 2021. Haematologica. Online ahead of print. PubMed
  14. Lee S, et al. 2021. Nature. 599:283. PubMed
  15. Marsden AJ, et al. 2021. ACS Pharmacol Transl Sci. 4:1567. PubMed
  16. Buijsers B, et al. 2022. Front Immunol. 12:759570. PubMed
  17. Garcia-Larragoiti N, et al. 2021. J Thromb Haemost. 19:2572. PubMed
  18. Petersen-Uribe Á, et al. 2021. Int J Mol Sci. 22:. PubMed
  19. Li L, et al. 2022. FEBS J. 289:2877. PubMed
  20. Kemble S, et al. 2022. Blood Adv. 6:2932. PubMed
  21. Li L, et al. 2023. Br J Pharmacol. 180:287. PubMed
  22. Cano-Mendez A, et al. 2022. Int J Mol Sci. 23: . PubMed
RRID
AB_314477 (BioLegend Cat. No. 304905)
AB_314478 (BioLegend Cat. No. 304906)

Antigen Details

Structure
Selectin, type I glycoprotein, 140 kD
Distribution

Activated platelets, megakaryocytes, endothelial cells

Function
Adhesion, neutrophil rolling, platelet-neutrophil and platelet-monocyte interactions
Ligand/Receptor
CD162 (PSGL-1), CD24 and sialylated Lewis X
Cell Type
Endothelial cells, Megakaryocytes, Neutrophils, Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. McEver R, et al. 1995. J. Biol. Chem. 270:11025.
2. Varki A. 1994. P. Natl. Acad. Sci. USA 91:7390.

Gene ID
6403 View all products for this Gene ID
UniProt
View information about CD62P on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account