Loading...

PE anti-human CD66b Antibody

Pricing & Availability
Clone
G10F5 (See other available formats)
Regulatory Status
RUO
Workshop
VI MA81
Other Names
CD67, CGM6, NCA-95, CEACAM8
Isotype
Mouse IgM, κ
Ave. Rating
Submit a Review
Product Citations
publications
G10F5_PE_040810
Human peripheral blood granulocytes stained with G10F5 PE
  • G10F5_PE_040810
    Human peripheral blood granulocytes stained with G10F5 PE
See PE spectral data
Cat # Size Price Quantity Check Availability Save
305105 25 tests 104€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
305106 100 tests 254€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen, formalin-fixed paraffin-embedded tissue sections, and spatial biology (IBEX)5,6.

Application References
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  3. Norling LV, et al. 2012. Arterioscler Thromb Vasc Biol. 32:1970. PubMed
  4. Meinke P, et al. 2015. Neuroimmunol Discord. 25:127. PubMed
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Wu L, et al. 2018. Oncol Lett. 15:9507. PubMed
  2. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  3. Butler AL, et al. 2019. Front Immunol. 10:1851. PubMed
  4. Crespo-Lessmann A, et al. 2017. J Asthma Allergy. 10:269. PubMed
  5. Miramón P, et al. 2012. PLoS One. 7:e52850. PubMed
  6. Schmidt T, et al. 2015. PLoS One. 10: 0132703. PubMed
  7. Inglis H, et al. 2015. J Vis Exp. 97: 52484. PubMed
  8. Khan E, et al. 2016. Sci Rep. 6:38104. PubMed
  9. O’Dea K, et al. 2016. PLoS One. 11:e0167801. PubMed
  10. Li X, et al. 2017. Stem Cell Res. 10.1016/j.scr.2017.03.014. PubMed
  11. Barbu EA, et al. 2020. Front Immunol. 1.385416667. PubMed
  12. Dong Y, et al. 2020. J Leukoc Biol. 108:1711. PubMed
  13. Klemm F, et al. 2020. Cell. 181(7):1643-1660.e17. PubMed
  14. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  15. Seman BG, et al. 2021. J Cell Sci. 134:. PubMed
  16. Barbu EA, et al. 2021. Bio Protoc. 11:e3927. PubMed
  17. Mol S, et al. 2021. Int J Mol Sci. 22:. PubMed
  18. Canals Hernaez D, et al. 2022. Front Oncol. 12:856424. PubMed
  19. Tähtinen S, et al. 2022. Nat Immunol. 23:532. PubMed
  20. Wang L, et al. 2022. Gut. Online ahead of print. PubMed
  21. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  22. Sehgal R, et al. 2022. Cells. 11: . PubMed
  23. García-García A, et al. 2023. Bioact Mater. 24:174. PubMed
  24. Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed
  25. Lareau CA, et al. 2023. Nat Protoc. 18:1416. PubMed
  26. Freund EC, et al. 2023. Methods Mol Biol. 2618:201. PubMed
  27. Doloff JC, et al. 2023. Sci Adv. 9:eade9488. PubMed
RRID
AB_10550093 (BioLegend Cat. No. 305105)
AB_2077857 (BioLegend Cat. No. 305106)

Antigen Details

Structure
Ig superfamily, CEA antigen group, GPI-linked glycoprotein, 95-100 kD
Distribution

Granulocytes

Function
Cell adhesion, neutrophil activation
Ligand/Receptor
CD66c
Cell Type
Granulocytes, Neutrophils
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kuijpers T, et al. 1993. J. Immunol. 151:4934.
2. Kuroki M, et al. 1992. J. Leuk. Biol. 52:551.

Gene ID
1088 View all products for this Gene ID
UniProt
View information about CD66b on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 01/05/2017

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account