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PE anti-human MICA/MICB Antibody

Pricing & Availability
Clone
6D4 (See other available formats)
Regulatory Status
RUO
Other Names
PERB11, MIC, MHC class I chain-related protein A, MICA, PERB11.1, MHC class I chain-related protein B, MICB, PERB11.2
Isotype
Mouse IgG2a, κ
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Product Citations
publications
6D4_PE_073007
Human Hela cell line stained with 6D4 PE
  • 6D4_PE_073007
    Human Hela cell line stained with 6D4 PE
See PE spectral data
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320906 100 tests 190€
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Description

6D4 antibody reacts with a common epitope of the human nonclassical MHC class I chain-related protein A (MICA) and B (MICB), also known as PERB11.1 and PERB11.2. The MIC gene is located in MHC class I region. MICA/B are 65-75 kD stress-inducible glycoproteins with highly polymorphic. They are MHC class I-like transmembrane molecules that do not associate β2-microglobulin and do not present peptides. MICA and MICB share 85% identify, and are mainly expressed on Intestinal epithelial cells, epithelial tumor cells, endothelial cells, fibroblasts, and IFN-α-stimulated dendritic cells. MIC molecules bind NKG2D, an activating receptor, and induce activation of NK cells, and subset of CD8+ α/β T cells and γ/δ T cells, as well as suppression of T cell proliferation. MICA/B recognition is involved in the regulation of tumor surveillance, viral infection and autoimmune diseases. The 6D4 antibody is able to block MIC mediated cytotoxicity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemistry2,3,5 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissue sections, immunoprecipitation7, and blocking2,3 of MIC mediated cytotoxicity. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 320919 & 320120).

Application References
  1. Groh V, et al. 1999. Science 279:1737.
  2. Groh V, et al. 1999. Proc. Natl. Acad. Sci. USA. 96:6879.
  3. Groh V, et al. 2001. Nature Immunol. 2:255.
  4. Li Z, et al. 2000. Immunogenetics 51:246.
  5. Park EJ, et al. 2003. J. Immunol. 171:4131.
  6. Jinushi M, et al. 2003. J. Immunol. 171:5423.
  7. Wu J, et al. 2003. J. Immunol. 170:4196.
Product Citations
  1. Godbersen C, et al. 2017. Mol Cancer Ther. 16:1335. PubMed
  2. Iwasa M, et al. 2019. Oncotarget. 10:1903. PubMed
  3. Tang S, et al. 2019. Biosci Rep. 39(7). PubMed
  4. Suzuki D, et al. 2020. Stem Cell Reports. 14:49. PubMed
  5. Iraqi M, et al. 2020. Front Cell Infect Microbiol. 10:48. PubMed
  6. Poznanski SM, et al. 2018. Cancer Immunol Res. 1.065277778. PubMed
  7. Watanabe M, et al. 2014. Int Immunol. 26:597. PubMed
  8. Kumashiro Y, et al. 2015. Small. 6: 681 - 687. PubMed
  9. Siew Y, et al. 2015. Int Immunol. 27: 621 - 632. PubMed
  10. Ritter C, et al. 2016. Sci Rep. 6:21678. PubMed
  11. Cordeau M, et al. 2016. PLoS One. 11:e0164401. PubMed
  12. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
  13. Portillo AL, et al. 2021. iScience. 24(6):102619. PubMed
  14. Hirano M, et al. 2021. J Exp Clin Cancer Res. 110:40. PubMed
  15. Okita R, et al. 2021. Thorac Cancer. 12:775. PubMed
  16. Van der Meer JMR, et al. 2021. Oncoimmunology. 10:1981049. PubMed
  17. Wang M, et al. 2022. Immun Inflamm Dis. 10:e626. PubMed
  18. Wang FM, et al. 2022. J Immunol Res. 2022:1786395. PubMed
RRID
AB_493193 (BioLegend Cat. No. 320906)

Antigen Details

Structure
65-75 kD glycoprotein, MHC class I-like transmembrane molecules that do not associate ß2-microglobulin, highly polymorphic
Distribution

Intestinal epithelium, epithelial tumors, endothelial cells, fibroblasts, INF-a-stimulated dendritic cells

Function
Activation of NK cells, subset of CD8+ α/ß T cells and γ/δ T cells, suppression of T cell proliferation
Ligand/Receptor
NKG2D
Cell Type
Dendritic cells, Endothelial cells, Epithelial cells, Fibroblasts
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Groh V, et al. 1996. Proc. Natl. Acad. Sci. USA. 93:12445.
2. Groh V, et al. 1999. Proc. Natl. Acad. Sci. USA. 96:6879.
3. Jinushi M, et al. 2003. J. Immunol. 170:1249.
4. Kriegeskorte AK, et al. 2005. Proc. Natl. Acad. Sci. USA. 102:11805.
5. Groh V, et al. 1999. Science 279:1737.

Gene ID
100507436 View all products for this Gene ID 4277 View all products for this Gene ID
UniProt
View information about MICA MICB on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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