Loading...

PE/Cyanine7 anti-human CD127 (IL-7Rα) Antibody

Pricing & Availability
Clone
A019D5 (See other available formats)
Regulatory Status
RUO
Other Names
IL-7 receptor α chain, IL-7Rα
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
A019D5_PECyanine7_CD127_Antibody_1_040924
Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) PE/Cyanine7 (left) or mouse IgG1 PE/Cyanine7 isotype control (right).
  • A019D5_PECyanine7_CD127_Antibody_1_040924
    Human peripheral blood lymphocytes were stained with CD3 FITC and CD127 (clone A019D5) PE/Cyanine7 (left) or mouse IgG1 PE/Cyanine7 isotype control (right).
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
351319 25 tests 141€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
351320 100 tests 292€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human CD127
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported (for the relevant formats) application: proteogenomics1.

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References
  1. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Lundtoft C, et al. 2017. PLoS Pathogens. 13(6):e1006425. PubMed
  2. Idorn M, et al. 2018. Oncoimmunology. 7:e1412029. PubMed
  3. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  4. Nicholas DA et al. 2019. Cell Metab. 30(3):447-461 . PubMed
  5. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  6. Yang M, et al. 2019. Cell Physiol Biochem. 52:1178. PubMed
  7. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  8. Catapano M, et al. 2020. J Invest Dermatol. 140:816. PubMed
  9. Saraiva DP, et al. 2018. Front Immunol. 2.184027778. PubMed
  10. Mashiko S, et al. 2015. J Allergy Clin Immunol. 136: 351-359. PubMed
  11. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  12. Withers D, et al. 2016. Nat Med. 22:319-23. PubMed
  13. Waddington KE, et al. 2020. Front Immunol. 1.51875. PubMed
  14. Nasrallah R, et al. 2020. Nature. 583:447. PubMed
  15. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  16. Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed
  17. Goc J, et al. 2021. Cell. 184:5015. PubMed
  18. Knoop J, et al. 2020. Diabetes. 661:69. PubMed
  19. Shafiei-Jahani P, et al. 2021. Nat Commun. 12:2526. PubMed
  20. Hardman CS, et al. 2021. Sci Immunol. 6:. PubMed
  21. Turner JA, et al. 2020. Immunity. 53:1202. PubMed
  22. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
  23. Wu X, et al. 2023. iScience. 26:106559. PubMed
  24. Urak R, et al. 2023. Cancers (Basel). 15:. PubMed
RRID
AB_10899414 (BioLegend Cat. No. 351319)
AB_10897098 (BioLegend Cat. No. 351320)

Antigen Details

Structure
Type I transmembrane glycoprotein, associates with CD132, 60-90 kD
Distribution

Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells

Function
T cell and immature B cell proliferation and development
Ligand/Receptor
IL-7
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503.
3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610.
4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.
5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.
6. Liu W, et al. 2006. J. Exp. Med. 203:1701.

Gene ID
3575 View all products for this Gene ID
UniProt
View information about CD127 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account