PE/Cyanine7 anti-human CD15 (SSEA-1) Antibody

Pricing & Availability
Clone
W6D3 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
Lewis X, 3-FAL, 3-FL, LNFP III, Lex , SSEA-1, X-hapten
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
W6D3_PeCy7_CD15_Antibody_FC_101712
Human peripheral blood granulocytes were stained with CD15 (clone W6D3) PE/Cyanine7 (filled histogram) or mouse IgG1, κ PE/Cyanine7 isotype control (open histogram).
  • W6D3_PeCy7_CD15_Antibody_FC_101712
    Human peripheral blood granulocytes were stained with CD15 (clone W6D3) PE/Cyanine7 (filled histogram) or mouse IgG1, κ PE/Cyanine7 isotype control (open histogram).
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
323029 25 tests 118€
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323030 100 tests 306€
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Description

CD15 is 3-fucosyl-N-acetyllactosamine (3-FAL) also known as Lewis X, 3-FAL, X-hapten, and SSEA-1. CD15 is expressed on granulocytes and monocytes. It has also been shown to be expressed on Langerhans cells. CD15 has been implicated in adhesion as well as chemotaxis, phagocytosis, and bactericidal activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
WERI-RB-1 retinoblastoma cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Product Citations
  1. Kubo M, et al. 2018. Oncol Rep. 39:417. PubMed
  2. Xia Y, et al. 2019. Gastroenterol Res Pract. 2019:5436961. PubMed
  3. Ostendorf L, et al. 2019. Front Immunol. 2.309027778. PubMed
  4. Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed
  5. Hultmark S, et al. 2020. Haematologica. . PubMed
  6. Ren X, et al. 2021. Cell Death Dis. 12:484. PubMed
  7. Martín Monreal MT, et al. 2021. Front Immunol. 12:716250. PubMed
  8. Moreno-Fernandez ME, et al. 2021. STAR Protoc. 2:100937. PubMed
  9. Haller PM, et al. 2020. Biomedicines. 8:. PubMed
  10. Ren K, et al. 2022. Brain Behav. 12:e2456. PubMed
RRID
AB_2561669 (BioLegend Cat. No. 323029)
AB_2561670 (BioLegend Cat. No. 323030)

Antigen Details

Structure
Poly-N-acetyllactosamine
Distribution

Neutrophils, eosinophils, monocytes

Function
Adhesion
Cell Type
Embryonic Stem Cells, Eosinophils, Neutrophils
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Stocks SC, et al. 1990. Biochem. J. 268:275.

Gene ID
2526 View all products for this Gene ID
UniProt
View information about CD15 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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