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PE/Cyanine7 anti-mouse Ly-6A/E (Sca-1) Antibody

Pricing & Availability
Clone
D7 (See other available formats)
Regulatory Status
RUO
Other Names
Sca-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
D7_PECy7_091207
C57BL/6 mouse splenocytes stained with D7 PE/Cyanine7
  • D7_PECy7_091207
    C57BL/6 mouse splenocytes stained with D7 PE/Cyanine7
See PE/Cyanine7 spectral data
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108113 25 µg 82€
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108114 100 µg 253€
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Description

Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent mouse T-cell line (CTL-L)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The Ly-6A/E antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The D7 antibody has been reported to induce T cell activation and inhibit TCR-induced IL-2 production. Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, in vitro lymphocyte activation3-6, induction of redirected lysis7, induction of T cell inhibitory signalling8, immunofluorescence9, and immunohistochemical staining of acetone-fixed frozen sections13 and Bouin-fixed, paraffin-embedded samples9.

The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.14 

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Ortega G, et al. 1986. J. Immunol. 137:3240. (WB, IP)
  2. Palfree RGE, et al. 1986. Immunogenetics 23:197. (WB)
  3. Codias EK, et al. 1990. J. Immunol. 144:2197.
  4. Malek TR, et al. 1986. J. Exp. Med. 164:709.
  5. Codias EK, et al. 1990. J. Immunol. 145:1407.
  6. Ivanov V, et al. 1994. J. Immunol. 153:2394.
  7. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  8. Fleming T, et al. 1994. J. Immunol. 153:1955.
  9. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IF, IHC)
  10. Umland O, et al. 2007. J. Immunol. 178:4147.
  11. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 45:149. (FC) PubMed
  12. Pronk CJ, et al. 2011. J. Exp Med. PubMed
  13. English A, et al. 2000. J. Immunol. 165:3763. (IHC)
  14. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
  15. Wiesner DL, et al. 2015. PLoS Pathog. 11:1004701. PubMed
Product Citations
  1. Del Barco Barrantes I, et al. 2018. Stem Cell Reports. 10:257. PubMed
  2. Youssif C, et al. 2018. EMBO Mol Med. 10:e8403. PubMed
  3. Sevin M, et al. 2018. Nat Commun. 9:1431. PubMed
  4. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
  5. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  6. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  7. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  8. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  9. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  10. Solanas G, et al. 2017. Cell. 170:678. PubMed
  11. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
  12. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  13. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
  14. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  15. Duan Y, et al. 2018. Stem Cells. 36:1430. PubMed
  16. Barman PK, et al. 2019. J Immunol. 202:2720. PubMed
  17. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  18. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  19. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  20. Telford W, et al. 2017. Cytometry A. 91:314. PubMed
  21. Gross ETE, et al. 2018. Oncoimmunology. 8:e1404212. PubMed
  22. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  23. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  24. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  25. Stifter SA, et al. 2019. Cell Rep. 29:3539. PubMed
  26. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  27. Lang J, et al. 2020. Nat Commun. 11:1338. PubMed
  28. Jin J, et al. 2020. Cell Reports. 30(12):4124-4136. PubMed
  29. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  30. Smith E, et al. 2009. J Immunol. 183:5685. PubMed
  31. Christoforou N, et al. 2013. PLoS One. 8:65963. PubMed
  32. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
  33. Gough D, et al. 2014. Blood. 124:2252. PubMed
  34. Giraddi R, et al. 2015. Nat Commun. 6: 8487. PubMed
  35. Yosef R, et al. 2016. Nat Commun. 7: 11190. PubMed
  36. Aryal B, et al. 2016. Nat Commun. 7:12313. PubMed
  37. Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
  38. García-Prat L, Muñoz-Cánoves P, Martínez-Vicente 2017. Muscle Stem Cells. 10.1007/978-1-4939-6771-1_14. PubMed
  39. Jin Y, et al. 2020. PeerJ. 8:e10374. PubMed
  40. Zaro BW, et al. 2020. eLife. 9:00. PubMed
  41. Kang YA, et al. 2020. J Exp Med. 217:00:00. PubMed
  42. Iwanami N, et al. 2020. iScience. 23:101260. PubMed
  43. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  44. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  45. Zhang C, et al. 2020. Cell Rep. 32:108206. PubMed
  46. Lerbs T, et al. 2020. JCI Insight. 5:00. PubMed
  47. Azazmeh N, et al. 2020. Nat Commun. 2.340972222. PubMed
  48. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
  49. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  50. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  51. Arai F, et al. 2020. Cell Systems. 11(6):640-652.e5. PubMed
  52. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  53. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  54. Bota-Rabassedas N, et al. 2021. Cell Reports. 35(3):109009. PubMed
  55. DellOrso S, et al. 2021. STAR Protocols. 2(2):100451. PubMed
  56. Liu F, et al. 2021. Cell Reports. 35(10):109225. PubMed
  57. Ankawa R, et al. 2021. Developmental Cell. . PubMed
  58. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  59. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  60. Kim SP, et al. 2021. Cell Reports. 36(9):109626. PubMed
  61. Onai N, et al. 2020. Int J Mol Sci. :22. PubMed
  62. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
  63. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  64. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
  65. Dong S, et al. 2021. Nature. 591:117. PubMed
  66. Campanario S, et al. 2021. Front Cell Dev Biol. 8:620409. PubMed
  67. Kang M, et al. 2021. Front Cell Dev Biol. 8:596622. PubMed
  68. Cho K, et al. 2021. iScience. 24:103117. PubMed
  69. Man N, et al. 2021. JCI Insight. 6:. PubMed
  70. Zhang B, et al. 2021. Nature. 599:471. PubMed
  71. Shen Y, et al. 2021. Comput Struct Biotechnol J. 19:5360. PubMed
  72. Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
  73. Beauchamp EM, et al. 2021. Blood Cancer Discov. 6:. PubMed
  74. Zheng Z, et al. 2021. Nat Commun. 12:6202. PubMed
  75. Wang S, et al. 2021. Commun Biol. 22:. PubMed
  76. Fielding C, et al. 2022. Nat Commun. 13:543. PubMed
  77. Chavez JS, et al. 2022. Cells. 11:. PubMed
  78. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  79. Hacein-Bey-Abina S, et al. 2020. Exp Hematol. 88:15. PubMed
  80. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
  81. Gutiérrez-Gutiérrez Ó, et al. 2021. EMBO Rep. 22:e52905. PubMed
  82. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
  83. Wu J, et al. 2022. Stem Cells Int. 2022:4249843. PubMed
  84. Dagher T, et al. 2021. J Exp Med. 218:. PubMed
  85. Hoffman D, et al. 2021. Immunity. 54:2712. PubMed
  86. Yoshinaga M, et al. 2022. Nat Commun. 13:6435. PubMed
  87. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  88. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
  89. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  90. Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed
  91. Moiseeva V, et al. 2023. Nature. 613:169. PubMed
  92. Huang D, et al. 2023. Exp Hematol Oncol. 12:11. PubMed
  93. Gomez-Salinero JM, et al. 2022. Cell Stem Cell. 29:593. PubMed
  94. Hong X, et al. 2022. Cell Stem Cell. 29:1298. PubMed
  95. Pioli KT, et al. 2023. iScience. 26:106223. PubMed
  96. Bobadilla Muñoz M, et al. 2023. Front Cell Dev Biol. 11:1128534. PubMed
RRID
AB_493597 (BioLegend Cat. No. 108113)
AB_493596 (BioLegend Cat. No. 108114)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution

Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells

Function
Regulates B and T cell responses
Cell Type
B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.

Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A/E on UniProt.org
Go To Top Version: 3    Revision Date: 07/11/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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