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Purified anti-β-actin Antibody

Pricing & Availability
Clone
2F1-1 (See other available formats)
Regulatory Status
RUO
Other Names
Actin, cytoplasmic 1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A_2F1-1_Purified_Beta-Actin_Antibody_1_WB_080316
Total cell lysate from HeLa cells (lane 1, 15 µg), NIN3T3 cells (lane 2, 15 µg), U87-MG (lane 3, 15 µg), mouse brain (lane 4, 15 µg) and rat brain (lane 5, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-β actin antibody (clone 2F1-1). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection.
  • A_2F1-1_Purified_Beta-Actin_Antibody_1_WB_080316
    Total cell lysate from HeLa cells (lane 1, 15 µg), NIN3T3 cells (lane 2, 15 µg), U87-MG (lane 3, 15 µg), mouse brain (lane 4, 15 µg) and rat brain (lane 5, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-β actin antibody (clone 2F1-1). Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection.
  • B_2F1-1_PURE_BetaActin_Ab_IF_022018
    Day-three cultured postnatal C57BL/6 mouse brain cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 2.5 µg/ml of purified anti- β-actin (clone 2F1-1) in blocking buffer, overnight at 4°C, followed by Alexa Fluor® 594 anti-mouse IgG (red) staining for 2 hours at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured with 40X objective.
  • C_2F1-1_PURE_Beta_Actin_Antibody_052022
    IHC staining using purified anti-β-actin antibody (clone 2F1-1) on formalin-fixed paraffin-embedded human kidney tissue. The tissue was incubated with 5 μg/mL of anti-β-actin antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-mouse IgG antibody (red) (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40x objective. Scalebar = 50 μM.
Cat # Size Price Quantity Check Availability Save
643801 25 µg 81€
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643802 100 µg 184€
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Description

β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Mouse,Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µL per 5 mL antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg/mL is recommended. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The binding of this antibody to its target is sensitive to salt concentration. For consistent results, please use TBS/T buffer for Western blotting that contains 0.15 M NaCl as indicated in the BioLegend recommended protocol. 

Application References
  1. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  2. Joyce CW, et al. 2006. J. Biol. Chem. 281:33053.
  3. Yanagiya T,et al.2007.Obesity.15:572.
  4. KishidaT,et al. 2007.J. Immunol. 179:8554.
  5. Ouimet M, et al. 2008. Arterisocler Thromb Vasc Biol. 28:1144.
  6. Toltlt LJ,et al. 2008.J. Immunol. 181:2165.
  7. Sawada. T,et al. 2008. J. Biol. Chem. 283:26820.
  8. Ikeda D, et al. 2008. Endocrinology. 149:6037.
  9. Ikeda Y, et al. 2010. Mol Endocrinol. 24:1338. PubMed
  10. Kaieda S, et al. 2010. J. Biol Chem. 285:21478. PubMed
  11. Cambos M, et al. 2011. J. Leukoc. Biol. 89:157. PubMed
Product Citations
  1. Qin S, et al. 2018. Oncol Lett. 128:1283. PubMed
  2. Hadjicharalambous MR, et al. 2019. Sci Rep. 9:6020. PubMed
  3. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  4. Budida R, et al. 2017. Eur J Immunol. 47:1819. PubMed
  5. Lalor R, et al. 2019. Nutrients. 11. PubMed
  6. Lovatt M, et al. 2018. Antioxidants (Basel). 0.416666667. PubMed
  7. Wagner AK, et al. 2017. Nat Commun. 8:15627. PubMed
  8. Kakaradov B, et al. 2017. Nat Immunol. 18:422. PubMed
  9. Kaieda S, et al. 2010. J Biol Chem. 285:21478. PubMed
  10. Ikeda Y, et al. 2010. Mol Endocrinol. 24:1338. PubMed
  11. Scorza M 2011. J Leukoc Biol. 89:159. PubMed
  12. Greenberg D, et al. 2015. Infect Immun . 83: 4277 - 4292. PubMed
  13. Bruggen R, et al. 2015. PLoS One. 10: 0144555. PubMed
  14. Callahan S, et al. 2020. Cell Microbiol. 22:e13210. PubMed
  15. Sims S, et al. 2021. Adv Virol. 2021:5569844. PubMed
  16. Xu Y, et al. 2022. Nat Commun. 13:980. PubMed
  17. Lai CC, et al. 2022. Biomed Rep. 16:19. PubMed
  18. Rodríguez E, et al. 2022. Commun Biol. 5:41. PubMed
  19. Hsiao CC, et al. 2021. Cells. 10:. PubMed
  20. Callahan SM, et al. 2021. Curr Protoc. 1:e294. PubMed
  21. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  22. Kaya-Sezginer E, et al. 2022. Andrology. 10:1441. PubMed
  23. Herr SA, et al. 2022. Neural Regen Res. 17:1505. PubMed
  24. Fathi M, et al. 2023. iScience. 26:106482. PubMed
  25. Tetikoglu S, et al. 2023. Chem Biodivers. 20:e202300401. PubMed
RRID
AB_2223201 (BioLegend Cat. No. 643801)
AB_2223199 (BioLegend Cat. No. 643802)

Antigen Details

Structure
Actin, cytoplasmic 1
Distribution

Ubiquitously expressed in the cytoplasm of all eukaryotic cells

Function
Actins are highly conserved proteins that are involved in cell motility
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microfilaments
Antigen References

1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673.
2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133.
3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.

Gene ID
11461 View all products for this Gene ID
UniProt
View information about beta-actin on UniProt.org

Related FAQs

There are no FAQs for this product.
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